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The map label for this gene is speB [C]

Identifier: 148642936

GI number: 148642936

Start: 832294

End: 833169

Strand: Reverse

Name: speB [C]

Synonym: Msm_0876

Alternate gene names: 148642936

Gene position: 833169-832294 (Counterclockwise)

Preceding gene: 148642937

Following gene: 148642935

Centisome position: 44.96

GC content: 34.13

Gene sequence:

>876_bases
ATGCTTTTAAACACATATGAACCATGGAAATTTGCTTTTTCCCAGGAAGAAACTGATTTTAACAACCTTAAAGAGGGTGC
TTGGGGAATAATTGGTGTTCCGTTTGACAGTACAACATCTTATCATCCTGGTTCACGTTTTGGACCCATAGTAGTTAGAG
AAGCTTCCTATGGGTTTGAAAAATATAATACTATTTTTAATACACAATTGGACAATATTTTTTACGATTTCGGAGACGTA
AATGTTGTTTTTGGAAACTGTAAAAAAACATGTGACATAATTGAAGATACTGTTAACGAACTGTCTGATTTAAAAATCAA
ACCTCTGACTATTGGTGGAGAACATAGTTTGACTATTGGTGTTCTTAATTCATTAACTAAAAAATATGATGATCTGACTG
TTGTCCATTTGGATGCTCATAGGGATCTGGCAGATACTTTTATTGGAGAGCTGTATTCCCATGCCAGTGTCATGAAAAGG
GTTCATGAAATGGGTGTTAAGGAATTGGTTCAGATTGGCATCAGGTCAGCTTCAAAGGAAGAGGAAGATTTTGTTAAAAA
CCAGTCAAATATCACAACATTTAAAAATAATGATGTATTTCATCATCTGGATAATATAGAATATTATTTGAGTATTATTG
ACACTCCAATTTATTTGTCTATTGATATGGATGTTTTTGATCCGTCTATTGCTCCGACTGTAGGCAACCCAACTCCCAAC
GGAATAGCGTATGGCCATATAGAAGCTATGCTTCAAACTTTATCTTTAAAAAATGTTGTCGGGATGGATGTTGTTGAAAC
AGCTGGTGACAGATTGGGAGATATTACTGCAGTTTCCGCTTCAAAAATAATTTATGATTTTTTATCATTATTGTGA

Upstream 100 bases:

>100_bases
CCAAATCACTGAAGGTATTGAAGTAGATTACATCGTAGCTTTAGGAAATATGAAAATTATGAGAACTAAATAATTTTATT
TAGTTCTTTTTTGATTTATT

Downstream 100 bases:

>100_bases
TAAATTGTTTTTACTTTTAATTTAATTTAACTTTTATTATTTTTTGTTCATTATTTTTTAAAATTTTTAGTTAATTATTA
CTTTTCTTATAAAAAATTAA

Product: arginase/agmatinase/formimionoglutamate hydrolase, SpeB

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MLLNTYEPWKFAFSQEETDFNNLKEGAWGIIGVPFDSTTSYHPGSRFGPIVVREASYGFEKYNTIFNTQLDNIFYDFGDV
NVVFGNCKKTCDIIEDTVNELSDLKIKPLTIGGEHSLTIGVLNSLTKKYDDLTVVHLDAHRDLADTFIGELYSHASVMKR
VHEMGVKELVQIGIRSASKEEEDFVKNQSNITTFKNNDVFHHLDNIEYYLSIIDTPIYLSIDMDVFDPSIAPTVGNPTPN
GIAYGHIEAMLQTLSLKNVVGMDVVETAGDRLGDITAVSASKIIYDFLSLL

Sequences:

>Translated_291_residues
MLLNTYEPWKFAFSQEETDFNNLKEGAWGIIGVPFDSTTSYHPGSRFGPIVVREASYGFEKYNTIFNTQLDNIFYDFGDV
NVVFGNCKKTCDIIEDTVNELSDLKIKPLTIGGEHSLTIGVLNSLTKKYDDLTVVHLDAHRDLADTFIGELYSHASVMKR
VHEMGVKELVQIGIRSASKEEEDFVKNQSNITTFKNNDVFHHLDNIEYYLSIIDTPIYLSIDMDVFDPSIAPTVGNPTPN
GIAYGHIEAMLQTLSLKNVVGMDVVETAGDRLGDITAVSASKIIYDFLSLL
>Mature_291_residues
MLLNTYEPWKFAFSQEETDFNNLKEGAWGIIGVPFDSTTSYHPGSRFGPIVVREASYGFEKYNTIFNTQLDNIFYDFGDV
NVVFGNCKKTCDIIEDTVNELSDLKIKPLTIGGEHSLTIGVLNSLTKKYDDLTVVHLDAHRDLADTFIGELYSHASVMKR
VHEMGVKELVQIGIRSASKEEEDFVKNQSNITTFKNNDVFHHLDNIEYYLSIIDTPIYLSIDMDVFDPSIAPTVGNPTPN
GIAYGHIEAMLQTLSLKNVVGMDVVETAGDRLGDITAVSASKIIYDFLSLL

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=271, Percent_Identity=30.9963099630996, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1789306, Length=269, Percent_Identity=29.368029739777, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32539; Mature: 32539

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNTYEPWKFAFSQEETDFNNLKEGAWGIIGVPFDSTTSYHPGSRFGPIVVREASYGFE
CCCCCCCCHHEECCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHH
KYNTIFNTQLDNIFYDFGDVNVVFGNCKKTCDIIEDTVNELSDLKIKPLTIGGEHSLTIG
HHHHHHHCHHHHHEEECCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHH
VLNSLTKKYDDLTVVHLDAHRDLADTFIGELYSHASVMKRVHEMGVKELVQIGIRSASKE
HHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEDFVKNQSNITTFKNNDVFHHLDNIEYYLSIIDTPIYLSIDMDVFDPSIAPTVGNPTPN
HHHHHHCCCCEEEECCCCHHHHHCCHHEEEEEECCCEEEEEECHHCCCCCCCCCCCCCCC
GIAYGHIEAMLQTLSLKNVVGMDVVETAGDRLGDITAVSASKIIYDFLSLL
CEEHHHHHHHHHHHHHHHHHCHHHHHHCCCHHCCEEHHHHHHHHHHHHHHC
>Mature Secondary Structure
MLLNTYEPWKFAFSQEETDFNNLKEGAWGIIGVPFDSTTSYHPGSRFGPIVVREASYGFE
CCCCCCCCHHEECCCCCCCHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHH
KYNTIFNTQLDNIFYDFGDVNVVFGNCKKTCDIIEDTVNELSDLKIKPLTIGGEHSLTIG
HHHHHHHCHHHHHEEECCCEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHH
VLNSLTKKYDDLTVVHLDAHRDLADTFIGELYSHASVMKRVHEMGVKELVQIGIRSASKE
HHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
EEDFVKNQSNITTFKNNDVFHHLDNIEYYLSIIDTPIYLSIDMDVFDPSIAPTVGNPTPN
HHHHHHCCCCEEEECCCCHHHHHCCHHEEEEEECCCEEEEEECHHCCCCCCCCCCCCCCC
GIAYGHIEAMLQTLSLKNVVGMDVVETAGDRLGDITAVSASKIIYDFLSLL
CEEHHHHHHHHHHHHHHHHHCHHHHHHCCCHHCCEEHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2377617 [H]