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The map label for this gene is hgiDIIM [H]

Identifier: 148642591

GI number: 148642591

Start: 507818

End: 508564

Strand: Reverse

Name: hgiDIIM [H]

Synonym: Msm_0531

Alternate gene names: 148642591

Gene position: 508564-507818 (Counterclockwise)

Preceding gene: 148642595

Following gene: 148642589

Centisome position: 27.44

GC content: 28.92

Gene sequence:

>747_bases
TTGAAAAAAGCCTATAAAATTTTACAGACTCATGAAAATCAAATAATAAATTTCAAGGATTATGGAGCGAACAGCAGTCG
TACCCGTTCAGTTACAATTGGTGTTAGAAGAGATTTAATTGATAAAGTACATCCTTTAGATTTATTTCCAGATAAAGAAG
AACCCAAAACTCTTATTGAAGTCATTGGTAATTTATCTAGTCTGAATGAAATGGGAGAAATTGATCCTTCTGACATATAT
CATCATTTTAAGCCCTATAGAGAAGATATGAGAGCATGGATTCATGATATTTCCGAAGGTGAATCTGCATTTGATAATGA
AGACATTAATAAAAGACCCCATAAAATTGTTGATGGAGAAATTGTTGTTCATAATAACAAACATGGTGATAAATATACAA
GACAATGTTGGGATAAAGTTGGTCCTTGTGTGCATACTTATATGGCTAATCTAGCGAGTCAAAATACAGTCCATCCAGTT
GATGATAGAGCATTTTCAATACATGAATTATTATTATTAATGAATATTCCTAATAATTTTAAATGGTCTGAAATAAGTGA
AGAAGAATTAAATAATTTACCATTAGAAGAAAAACAACAATTTTTAAAGGAAAATGAAGCAAATATAAGAGAATGTATTG
GGGAAGCTGTTCCAACTATAATAATGCAAAAAATTGCAAAGAATATTAAAAAAGTATTGATAACTGGAAAAAAATCTCAA
AAAAAAGGACAAACACGTTTAATCTAA

Upstream 100 bases:

>100_bases
TTTAAGTAATTTACTATTTTTAAATCAATATTTTTTAAATTAAAGCAAAATTAGAACGAATTTTCATCGAGGTGAAGTTT
CAATTGTAAAAAAAATTAAT

Downstream 100 bases:

>100_bases
TGGACTAAATTTTCATTCAAAATATTAATTTTATTTCCAGTTCATTTAATTATTCTTCCAATAGGAAACTGTATTTTCAC
ATATCTTTCTAAATAAAATA

Product: adenine/cytosine DNA methyltransferase

Products: NA

Alternate protein names: M.HgiDII; Cytosine-specific methyltransferase HgiDII [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKKAYKILQTHENQIINFKDYGANSSRTRSVTIGVRRDLIDKVHPLDLFPDKEEPKTLIEVIGNLSSLNEMGEIDPSDIY
HHFKPYREDMRAWIHDISEGESAFDNEDINKRPHKIVDGEIVVHNNKHGDKYTRQCWDKVGPCVHTYMANLASQNTVHPV
DDRAFSIHELLLLMNIPNNFKWSEISEEELNNLPLEEKQQFLKENEANIRECIGEAVPTIIMQKIAKNIKKVLITGKKSQ
KKGQTRLI

Sequences:

>Translated_248_residues
MKKAYKILQTHENQIINFKDYGANSSRTRSVTIGVRRDLIDKVHPLDLFPDKEEPKTLIEVIGNLSSLNEMGEIDPSDIY
HHFKPYREDMRAWIHDISEGESAFDNEDINKRPHKIVDGEIVVHNNKHGDKYTRQCWDKVGPCVHTYMANLASQNTVHPV
DDRAFSIHELLLLMNIPNNFKWSEISEEELNNLPLEEKQQFLKENEANIRECIGEAVPTIIMQKIAKNIKKVLITGKKSQ
KKGQTRLI
>Mature_248_residues
MKKAYKILQTHENQIINFKDYGANSSRTRSVTIGVRRDLIDKVHPLDLFPDKEEPKTLIEVIGNLSSLNEMGEIDPSDIY
HHFKPYREDMRAWIHDISEGESAFDNEDINKRPHKIVDGEIVVHNNKHGDKYTRQCWDKVGPCVHTYMANLASQNTVHPV
DDRAFSIHELLLLMNIPNNFKWSEISEEELNNLPLEEKQQFLKENEANIRECIGEAVPTIIMQKIAKNIKKVLITGKKSQ
KKGQTRLI

Specific function: This methylase recognizes the double-stranded sequence GTCGAC, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the HgiDII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 28693; Mature: 28693

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAYKILQTHENQIINFKDYGANSSRTRSVTIGVRRDLIDKVHPLDLFPDKEEPKTLIE
CCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHH
VIGNLSSLNEMGEIDPSDIYHHFKPYREDMRAWIHDISEGESAFDNEDINKRPHKIVDGE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEECCE
IVVHNNKHGDKYTRQCWDKVGPCVHTYMANLASQNTVHPVDDRAFSIHELLLLMNIPNNF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
KWSEISEEELNNLPLEEKQQFLKENEANIRECIGEAVPTIIMQKIAKNIKKVLITGKKSQ
CCCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KKGQTRLI
CCCCCCCC
>Mature Secondary Structure
MKKAYKILQTHENQIINFKDYGANSSRTRSVTIGVRRDLIDKVHPLDLFPDKEEPKTLIE
CCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCHHHHHH
VIGNLSSLNEMGEIDPSDIYHHFKPYREDMRAWIHDISEGESAFDNEDINKRPHKIVDGE
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCEEECCE
IVVHNNKHGDKYTRQCWDKVGPCVHTYMANLASQNTVHPVDDRAFSIHELLLLMNIPNNF
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCC
KWSEISEEELNNLPLEEKQQFLKENEANIRECIGEAVPTIIMQKIAKNIKKVLITGKKSQ
CCCCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
KKGQTRLI
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1937045; 7607523 [H]