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The map label for this gene is tfb

Identifier: 148642484

GI number: 148642484

Start: 406267

End: 407199

Strand: Reverse

Name: tfb

Synonym: Msm_0424

Alternate gene names: 148642484

Gene position: 407199-406267 (Counterclockwise)

Preceding gene: 148642485

Following gene: 148642480

Centisome position: 21.97

GC content: 38.26

Gene sequence:

>933_bases
ATGCAAGGTGATGTTTATGATAAAGATAAACAAACCGTATGTCCGGAATGTGGTTCCACAGAATTAATTGGTGATTATGA
AAGGGCAGAAGTAGTTTGTGCTCACTGTGGATTAGTTATTGATGAAAATCTAGTGGATATGGGTCCTGAATGGAGGGCAT
TTGACCACGAACAAAGAGATAAACGTACTAGGGTTGGAGCTCCAATCACTTACACCATTCACGATAAAGGTTTAAGTACT
ATGATTGACTGGAGGAATAAGGACATTTATGGTCGTGATATTCCTGCAAGAAACAGAGCACAATGGTATAGATTAAGGAA
ATGGCAAAGAAAAATTAGGATTTCTGGTGCTACAGAACGTAACTTGGCATTTGCATTAAGTGAGCTGGACAGAGATTCTT
CAAGATTAGGTCTTCCAAGAAGTGTGAGGGAAGCTGCATCTGTAGTATACAGAAGTGCAGTGGACAACAAACTTATTCGT
GGAAGGAGTATTGAAGGAGTAGTTGCTGCTTCTTTATATGCTGCTTGCAGACGTTGTAATGTTCCCCGTACTTTAGATGA
AATAGCTGAAGTTTCAAGAGTTACTAAAAAAGAAGTAGGTAGGACTTACAGATTCCTTACAAGGGAATTAAACATTAAAT
TACCTCCAACTTCTCCGGTCGATTATGTGCCTAGATTTGCTAGTGAATTAGGTTTGTCTGGTGAAGCACAATCCAGAGCT
ATTGAAATTATTGAAAAAGCAATGGAAAAAGGTTTGACTTCTGGTAGAGGACCAACTGGTGTAGCTGCAGCTGCATTATA
TATTGCATCTGTATTGTTGGGTGAGAGAAAAACCCAAAGAGATGTAGCAGATATTGCAGGTGTTACTGAAGTAACTATAA
GAAACAGGTATAAAGAATTAACAGAACAACTTGAAATGGGTGTAACTTTATAA

Upstream 100 bases:

>100_bases
CAAAATCTAAGAAACCTAGGAAGAGGAGGGTGAAAACACGAAACAAAAAGTAAACGAAATCCCTAAAAAGGTCGGACATA
AAAGAACAGAAAAAGAAATT

Downstream 100 bases:

>100_bases
ACCACCCTATTTTAACTTTTTTTACTATATTTTTCAATACGATATATTAATTCATTGACAATTCCATCGTTATTTGTTTT
TTTAACTTTAGCTAGTTTTT

Product: transcription initiation factor IIB

Products: NA

Alternate protein names: TFIIB

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MQGDVYDKDKQTVCPECGSTELIGDYERAEVVCAHCGLVIDENLVDMGPEWRAFDHEQRDKRTRVGAPITYTIHDKGLST
MIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVREAASVVYRSAVDNKLIR
GRSIEGVVAASLYAACRRCNVPRTLDEIAEVSRVTKKEVGRTYRFLTRELNIKLPPTSPVDYVPRFASELGLSGEAQSRA
IEIIEKAMEKGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLEMGVTL

Sequences:

>Translated_310_residues
MQGDVYDKDKQTVCPECGSTELIGDYERAEVVCAHCGLVIDENLVDMGPEWRAFDHEQRDKRTRVGAPITYTIHDKGLST
MIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVREAASVVYRSAVDNKLIR
GRSIEGVVAASLYAACRRCNVPRTLDEIAEVSRVTKKEVGRTYRFLTRELNIKLPPTSPVDYVPRFASELGLSGEAQSRA
IEIIEKAMEKGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLEMGVTL
>Mature_310_residues
MQGDVYDKDKQTVCPECGSTELIGDYERAEVVCAHCGLVIDENLVDMGPEWRAFDHEQRDKRTRVGAPITYTIHDKGLST
MIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATERNLAFALSELDRDSSRLGLPRSVREAASVVYRSAVDNKLIR
GRSIEGVVAASLYAACRRCNVPRTLDEIAEVSRVTKKEVGRTYRFLTRELNIKLPPTSPVDYVPRFASELGLSGEAQSRA
IEIIEKAMEKGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKELTEQLEMGVTL

Specific function: Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre- initiation complex (DNA-TBP-TFIIB)

COG id: COG1405

COG function: function code K; Transcription initiation factor TFIIIB, Brf1 subunit/Transcription initiation factor TFIIB

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TFIIB-type zinc finger

Homologues:

Organism=Homo sapiens, GI4504193, Length=286, Percent_Identity=34.6153846153846, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI22035556, Length=291, Percent_Identity=26.8041237113402, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17564892, Length=288, Percent_Identity=36.4583333333333, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17531397, Length=302, Percent_Identity=25.1655629139073, Blast_Score=97, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325343, Length=306, Percent_Identity=30.0653594771242, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321685, Length=294, Percent_Identity=23.1292517006803, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136758, Length=297, Percent_Identity=34.006734006734, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI281361961, Length=302, Percent_Identity=27.1523178807947, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24647658, Length=302, Percent_Identity=27.1523178807947, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TF2B_METS3 (A5UKA1)

Other databases:

- EMBL:   CP000678
- RefSeq:   YP_001272997.1
- ProteinModelPortal:   A5UKA1
- STRING:   A5UKA1
- GeneID:   5216442
- GenomeReviews:   CP000678_GR
- KEGG:   msi:Msm_0424
- eggNOG:   arNOG04485
- HOGENOM:   HBG646536
- OMA:   HDYERAE
- ProtClustDB:   PRK00423
- HAMAP:   MF_00383
- InterPro:   IPR006670
- InterPro:   IPR011028
- InterPro:   IPR013763
- InterPro:   IPR000812
- InterPro:   IPR013150
- InterPro:   IPR013137
- Gene3D:   G3DSA:1.10.472.10
- PANTHER:   PTHR11618
- PRINTS:   PR00685
- SMART:   SM00385

Pfam domain/function: PF00382 TFIIB; PF08271 TFIIB_Zn_Ribbon; SSF47954 Cyclin_like

EC number: NA

Molecular weight: Translated: 34834; Mature: 34834

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS00782 TFIIB; PS51134 ZF_TFIIB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGDVYDKDKQTVCPECGSTELIGDYERAEVVCAHCGLVIDENLVDMGPEWRAFDHEQRD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH
KRTRVGAPITYTIHDKGLSTMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATER
HHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
NLAFALSELDRDSSRLGLPRSVREAASVVYRSAVDNKLIRGRSIEGVVAASLYAACRRCN
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
VPRTLDEIAEVSRVTKKEVGRTYRFLTRELNIKLPPTSPVDYVPRFASELGLSGEAQSRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCHHHHH
IEIIEKAMEKGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKEL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHH
TEQLEMGVTL
HHHHHCCCCC
>Mature Secondary Structure
MQGDVYDKDKQTVCPECGSTELIGDYERAEVVCAHCGLVIDENLVDMGPEWRAFDHEQRD
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHH
KRTRVGAPITYTIHDKGLSTMIDWRNKDIYGRDIPARNRAQWYRLRKWQRKIRISGATER
HHHHCCCCEEEEEECCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHH
NLAFALSELDRDSSRLGLPRSVREAASVVYRSAVDNKLIRGRSIEGVVAASLYAACRRCN
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
VPRTLDEIAEVSRVTKKEVGRTYRFLTRELNIKLPPTSPVDYVPRFASELGLSGEAQSRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCCHHHHH
IEIIEKAMEKGLTSGRGPTGVAAAALYIASVLLGERKTQRDVADIAGVTEVTIRNRYKEL
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHH
TEQLEMGVTL
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA