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The map label for this gene is hycI [C]

Identifier: 148642422

GI number: 148642422

Start: 345705

End: 346130

Strand: Reverse

Name: hycI [C]

Synonym: Msm_0362

Alternate gene names: 148642422

Gene position: 346130-345705 (Counterclockwise)

Preceding gene: 148642423

Following gene: 148642421

Centisome position: 18.68

GC content: 29.58

Gene sequence:

>426_bases
TTGATTATATTAGGGATTGGAAACGAGCTTAAATGTGATGATGGTGTAGGGCCTTTTATAATTAATGAATTAAAAGATTT
AGAAAATCCTAATTTAATAATCATTGATGGCAAGACAGTTCCTGAGAATTTCACAGGTAAAATTAGAAAAGAACAGCCTT
CACATGTAATTCTTGTTGATGCATGTTTAATGGGATGTAAACCTGGTGAATTTAAAATTGTAGACAAGGATGATTTTGTT
AATATTGGAATTTCAACTCATTCAATGTCTTTATCATATTTTGTTAAATATTTAGAGCGAGATAATGATTTTAAGATAAT
ATTTGTTGGTATTGAACCTGAATCCATGGATTATTCAGATAAGCCAACACCAGCAGTTCAAAAGGGAGCCTACAAGTTTA
TAAATATTTTGAAGGAGATTTTATGA

Upstream 100 bases:

>100_bases
TTATGGGAAAAGGAATTGGAACTATTGAAGCAAATTATTATTAGGTGATTTTATTTCATTTAAAAGACAGCTGGAAGAAT
TTTTAGAAAATAATGAAAAA

Downstream 100 bases:

>100_bases
AAATTTTATTTATCGGTTCAAGATTGTATGATGATATAGATTATTATGTTAGGGAAAACGGTATTGAAAGCATAATTACT
GAATCTAATGAAGATGCCAT

Product: coenzyme F420-reducing hydrogenase (Ni,Fe-hydrogenase maturation protease), delta subunit

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MIILGIGNELKCDDGVGPFIINELKDLENPNLIIIDGKTVPENFTGKIRKEQPSHVILVDACLMGCKPGEFKIVDKDDFV
NIGISTHSMSLSYFVKYLERDNDFKIIFVGIEPESMDYSDKPTPAVQKGAYKFINILKEIL

Sequences:

>Translated_141_residues
MIILGIGNELKCDDGVGPFIINELKDLENPNLIIIDGKTVPENFTGKIRKEQPSHVILVDACLMGCKPGEFKIVDKDDFV
NIGISTHSMSLSYFVKYLERDNDFKIIFVGIEPESMDYSDKPTPAVQKGAYKFINILKEIL
>Mature_141_residues
MIILGIGNELKCDDGVGPFIINELKDLENPNLIIIDGKTVPENFTGKIRKEQPSHVILVDACLMGCKPGEFKIVDKDDFV
NIGISTHSMSLSYFVKYLERDNDFKIIFVGIEPESMDYSDKPTPAVQKGAYKFINILKEIL

Specific function: Protease Involved In The C-Terminal Processing Of Hyce, The Large Subunit Of Hydrogenase 3. [C]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=114, Percent_Identity=36.8421052631579, Blast_Score=87, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: 1.12.98.1

Molecular weight: Translated: 15824; Mature: 15824

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILGIGNELKCDDGVGPFIINELKDLENPNLIIIDGKTVPENFTGKIRKEQPSHVILVD
CEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCCCCEEEEEE
ACLMGCKPGEFKIVDKDDFVNIGISTHSMSLSYFVKYLERDNDFKIIFVGIEPESMDYSD
HHHHCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
KPTPAVQKGAYKFINILKEIL
CCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIILGIGNELKCDDGVGPFIINELKDLENPNLIIIDGKTVPENFTGKIRKEQPSHVILVD
CEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECCCCCEEEEEE
ACLMGCKPGEFKIVDKDDFVNIGISTHSMSLSYFVKYLERDNDFKIIFVGIEPESMDYSD
HHHHCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCC
KPTPAVQKGAYKFINILKEIL
CCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]