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The map label for this gene is hycG [C]

Identifier: 148642373

GI number: 148642373

Start: 302748

End: 303197

Strand: Reverse

Name: hycG [C]

Synonym: Msm_0313

Alternate gene names: 148642373

Gene position: 303197-302748 (Counterclockwise)

Preceding gene: 148642374

Following gene: 148642372

Centisome position: 16.36

GC content: 38.67

Gene sequence:

>450_bases
ATGTTAAATGCTATTAAGGATGTTGTGAGGAAAAGCTCAATTCATGTTTGTATTGTGAATTGCGGAGGATGTAATGGGTG
TGATGTTGAACTGGTTGCATTAACTTCTCCAAGATATGATTTAGAGCAGTATGGTATTTATGTTCATAACAATCCTCGTG
AAGCTGATGTTTTATTATTGACTGGTGCTGTTACCGAGCAGTGGGTTGATAATTTAAAGAGAGTTTACGATAAAGCTCCT
GAACCAAAAATTGTAGTGGCTGTTGGAAATTGTCCTCAATCAGGAGATGTATTTAATCAGGAAGGTGGAAAAGTTTATGC
TCCTGCTTCTGATTTTATACCTGTTGATGCAGAGATACCTGGTTGCCCACCTAGACCTAGTGAAATTTTAGAGGCAATTT
TAGCTGTGGGTCCACAGGCTATTGCTAATCGTGGGAGGGAAAAGAAATGA

Upstream 100 bases:

>100_bases
TTGCTTTAGATGAGGGTAGAAAGGATTTATTTAATTTATTAAAGAATATAAATAATGAATAGTATAAATATAATAATTAA
TAATTTTGAGAGGCTTATAG

Downstream 100 bases:

>100_bases
TATTACCTATTGGTCCGATTCACCCATATTTAAAAGAACCGATAAGGCTAAAACTTCAGACCGAAGGAGAAAGAGTTGTT
AAAGCCGAAATTGAATATGG

Product: [NiFe]-hydrogenase-3-type complex, small subunit/NADH:quinone oxidoreductase (complex I), subunit PSST/NdhK/NuoB

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MLNAIKDVVRKSSIHVCIVNCGGCNGCDVELVALTSPRYDLEQYGIYVHNNPREADVLLLTGAVTEQWVDNLKRVYDKAP
EPKIVVAVGNCPQSGDVFNQEGGKVYAPASDFIPVDAEIPGCPPRPSEILEAILAVGPQAIANRGREKK

Sequences:

>Translated_149_residues
MLNAIKDVVRKSSIHVCIVNCGGCNGCDVELVALTSPRYDLEQYGIYVHNNPREADVLLLTGAVTEQWVDNLKRVYDKAP
EPKIVVAVGNCPQSGDVFNQEGGKVYAPASDFIPVDAEIPGCPPRPSEILEAILAVGPQAIANRGREKK
>Mature_149_residues
MLNAIKDVVRKSSIHVCIVNCGGCNGCDVELVALTSPRYDLEQYGIYVHNNPREADVLLLTGAVTEQWVDNLKRVYDKAP
EPKIVVAVGNCPQSGDVFNQEGGKVYAPASDFIPVDAEIPGCPPRPSEILEAILAVGPQAIANRGREKK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=133, Percent_Identity=33.0827067669173, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789074, Length=138, Percent_Identity=43.4782608695652, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1788834, Length=131, Percent_Identity=38.9312977099237, Blast_Score=103, Evalue=5e-24,
Organism=Escherichia coli, GI1788624, Length=136, Percent_Identity=33.0882352941176, Blast_Score=77, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17509685, Length=127, Percent_Identity=34.6456692913386, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI18859983, Length=114, Percent_Identity=34.2105263157895, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24642371, Length=114, Percent_Identity=34.2105263157895, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24651058, Length=114, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 16100; Mature: 16100

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNAIKDVVRKSSIHVCIVNCGGCNGCDVELVALTSPRYDLEQYGIYVHNNPREADVLLL
CCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHCCEEEECCCCCCEEEEE
TGAVTEQWVDNLKRVYDKAPEPKIVVAVGNCPQSGDVFNQEGGKVYAPASDFIPVDAEIP
ECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEECCCCCC
GCPPRPSEILEAILAVGPQAIANRGREKK
CCCCCHHHHHHHHHHCCCHHHHCCCCCCC
>Mature Secondary Structure
MLNAIKDVVRKSSIHVCIVNCGGCNGCDVELVALTSPRYDLEQYGIYVHNNPREADVLLL
CCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEECCCCCCHHHCCEEEECCCCCCEEEEE
TGAVTEQWVDNLKRVYDKAPEPKIVVAVGNCPQSGDVFNQEGGKVYAPASDFIPVDAEIP
ECHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCEECCCCCC
GCPPRPSEILEAILAVGPQAIANRGREKK
CCCCCHHHHHHHHHHCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]