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The map label for this gene is hycE [C]

Identifier: 148642372

GI number: 148642372

Start: 301627

End: 302751

Strand: Reverse

Name: hycE [C]

Synonym: Msm_0312

Alternate gene names: 148642372

Gene position: 302751-301627 (Counterclockwise)

Preceding gene: 148642373

Following gene: 148642371

Centisome position: 16.34

GC content: 34.4

Gene sequence:

>1125_bases
ATGATATTACCTATTGGTCCGATTCACCCATATTTAAAAGAACCGATAAGGCTAAAACTTCAGACCGAAGGAGAAAGAGT
TGTTAAAGCCGAAATTGAATATGGTTATGTTCACAGAGGGATTGAAAAGATAATTGAAGGTAAAACTTGGCAAAAAGGTA
TTTATTTGGCTGAAAGAGTTTGCGGAATATGTTCTTATGAACATACTCAATCATTTGCAGAAACTATTGAGAGAATCTCC
GGAGTTTATGCACCTTTAAGAGCTCAGTTTTTAAGGGTTATTACAAATGAATTAGACAGGATTCAAAGTCATTTGCTTGG
TAATTCTACATTTTTCAAATCTATTGATCATGAAACATTATATATGCAATCTTTGGATATAAGGGAATATGCAATGGATT
CTATTGAACTGTTAACTGGAAATAGAGTTAATATGGGTTGGAATGTAGTAGGTGGAGTTAAAATGGATGCTGATGAACGT
CATTTCAAACCGATTCTTGATAATCTGAAAATTATTGAAGAGAGAATTCCAAGATTAAGAGAGATTTATGCAGAAGGTCC
TGCTATTGGAATGCGTTCCCGTAATGTTGGAGTAATGACTAAAAAAGAAGCTATTAAAGGAAAAGCTGTAGGTCCTATTG
GAAGAGGATCCGGATTAAAGCATGATTTAAGGGAAGACCATTATACTTACAACGACCACTTTGATTTTAAAACCATCTGG
AGAAAAGAAGGGGACAATTATGCAAGAACCTTAAACAGGATTGATGAAATACCTGAGTGTATCAGTTTAATCAGGCAGGC
TATTGAAAACATGCCTAAAGGAGATATTCGTGTTCCTGTTGATATTAAATCAGGATATGCTGAATGGAGAAATGAAGCTC
CTCGTGGTGAAGTAAGTTATATGATTGAAACCAACGGTAATTTAATTAAACATATTTCCATCAGGACTCCAAGTATTTCA
AATATGGATTCCTGTGCAAAATATATGATTAAGGATGTTGCTACTGTTGCAGATGCAGTATCCACTTATGCATCAAGTGA
TCCTTGTGTTGCATGTGCAGAAAGAGTTGCTATTACTGATAAAAAAGGCAATACAACTACTAAAAATATTTTTGAGGTAA
GGTAA

Upstream 100 bases:

>100_bases
ATGCAGAGATACCTGGTTGCCCACCTAGACCTAGTGAAATTTTAGAGGCAATTTTAGCTGTGGGTCCACAGGCTATTGCT
AATCGTGGGAGGGAAAAGAA

Downstream 100 bases:

>100_bases
AATGTCTTCTTTGATGTGGTATATTTATGATTTTGCCAGAAAAGCATGGGCACAGGGATTTACTAATGCCAAAACCAGTC
CGGAAATTGTAGAAAAACCA

Product: [NiFe]-hydrogenase-3-type complex, large subunit/NADH:quinone oxidoreductase (complex I), subunit 49K/NdhH/NuoD

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MILPIGPIHPYLKEPIRLKLQTEGERVVKAEIEYGYVHRGIEKIIEGKTWQKGIYLAERVCGICSYEHTQSFAETIERIS
GVYAPLRAQFLRVITNELDRIQSHLLGNSTFFKSIDHETLYMQSLDIREYAMDSIELLTGNRVNMGWNVVGGVKMDADER
HFKPILDNLKIIEERIPRLREIYAEGPAIGMRSRNVGVMTKKEAIKGKAVGPIGRGSGLKHDLREDHYTYNDHFDFKTIW
RKEGDNYARTLNRIDEIPECISLIRQAIENMPKGDIRVPVDIKSGYAEWRNEAPRGEVSYMIETNGNLIKHISIRTPSIS
NMDSCAKYMIKDVATVADAVSTYASSDPCVACAERVAITDKKGNTTTKNIFEVR

Sequences:

>Translated_374_residues
MILPIGPIHPYLKEPIRLKLQTEGERVVKAEIEYGYVHRGIEKIIEGKTWQKGIYLAERVCGICSYEHTQSFAETIERIS
GVYAPLRAQFLRVITNELDRIQSHLLGNSTFFKSIDHETLYMQSLDIREYAMDSIELLTGNRVNMGWNVVGGVKMDADER
HFKPILDNLKIIEERIPRLREIYAEGPAIGMRSRNVGVMTKKEAIKGKAVGPIGRGSGLKHDLREDHYTYNDHFDFKTIW
RKEGDNYARTLNRIDEIPECISLIRQAIENMPKGDIRVPVDIKSGYAEWRNEAPRGEVSYMIETNGNLIKHISIRTPSIS
NMDSCAKYMIKDVATVADAVSTYASSDPCVACAERVAITDKKGNTTTKNIFEVR
>Mature_374_residues
MILPIGPIHPYLKEPIRLKLQTEGERVVKAEIEYGYVHRGIEKIIEGKTWQKGIYLAERVCGICSYEHTQSFAETIERIS
GVYAPLRAQFLRVITNELDRIQSHLLGNSTFFKSIDHETLYMQSLDIREYAMDSIELLTGNRVNMGWNVVGGVKMDADER
HFKPILDNLKIIEERIPRLREIYAEGPAIGMRSRNVGVMTKKEAIKGKAVGPIGRGSGLKHDLREDHYTYNDHFDFKTIW
RKEGDNYARTLNRIDEIPECISLIRQAIENMPKGDIRVPVDIKSGYAEWRNEAPRGEVSYMIETNGNLIKHISIRTPSIS
NMDSCAKYMIKDVATVADAVSTYASSDPCVACAERVAITDKKGNTTTKNIFEVR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260898743, Length=279, Percent_Identity=28.673835125448, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI4758786, Length=279, Percent_Identity=28.673835125448, Blast_Score=117, Evalue=1e-26,
Organism=Escherichia coli, GI1789076, Length=370, Percent_Identity=29.1891891891892, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788832, Length=372, Percent_Identity=29.3010752688172, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI145693162, Length=387, Percent_Identity=24.2894056847545, Blast_Score=129, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17555284, Length=372, Percent_Identity=27.4193548387097, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17568379, Length=372, Percent_Identity=27.1505376344086, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24638644, Length=371, Percent_Identity=24.7978436657682, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI221459469, Length=278, Percent_Identity=26.6187050359712, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 42538; Mature: 42538

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00535 COMPLEX1_49K ; PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILPIGPIHPYLKEPIRLKLQTEGERVVKAEIEYGYVHRGIEKIIEGKTWQKGIYLAERV
CCCCCCCCCHHHCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CGICSYEHTQSFAETIERISGVYAPLRAQFLRVITNELDRIQSHLLGNSTFFKSIDHETL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEE
YMQSLDIREYAMDSIELLTGNRVNMGWNVVGGVKMDADERHFKPILDNLKIIEERIPRLR
EHHHCCHHHHHHHHHHHHCCCEECCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHH
EIYAEGPAIGMRSRNVGVMTKKEAIKGKAVGPIGRGSGLKHDLREDHYTYNDHFDFKTIW
HHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHH
RKEGDNYARTLNRIDEIPECISLIRQAIENMPKGDIRVPVDIKSGYAEWRNEAPRGEVSY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCCEEE
MIETNGNLIKHISIRTPSISNMDSCAKYMIKDVATVADAVSTYASSDPCVACAERVAITD
EEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEC
KKGNTTTKNIFEVR
CCCCCCCCCEEECC
>Mature Secondary Structure
MILPIGPIHPYLKEPIRLKLQTEGERVVKAEIEYGYVHRGIEKIIEGKTWQKGIYLAERV
CCCCCCCCCHHHCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CGICSYEHTQSFAETIERISGVYAPLRAQFLRVITNELDRIQSHLLGNSTFFKSIDHETL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEE
YMQSLDIREYAMDSIELLTGNRVNMGWNVVGGVKMDADERHFKPILDNLKIIEERIPRLR
EHHHCCHHHHHHHHHHHHCCCEECCCCEEECCEEECCCHHHHHHHHHHHHHHHHHHHHHH
EIYAEGPAIGMRSRNVGVMTKKEAIKGKAVGPIGRGSGLKHDLREDHYTYNDHFDFKTIW
HHHHCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHH
RKEGDNYARTLNRIDEIPECISLIRQAIENMPKGDIRVPVDIKSGYAEWRNEAPRGEVSY
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCCCCEEE
MIETNGNLIKHISIRTPSISNMDSCAKYMIKDVATVADAVSTYASSDPCVACAERVAITD
EEECCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEEC
KKGNTTTKNIFEVR
CCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]