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The map label for this gene is 148642114

Identifier: 148642114

GI number: 148642114

Start: 50498

End: 51055

Strand: Reverse

Name: 148642114

Synonym: Msm_0054

Alternate gene names: NA

Gene position: 51055-50498 (Counterclockwise)

Preceding gene: 148642115

Following gene: 148642113

Centisome position: 2.76

GC content: 27.24

Gene sequence:

>558_bases
ATGTCTCAAATCAGATCTTTTTTAGCTATTGATTTAGATGATGATTTTAAACCTAAAGTAAATAAAATCATAAAAGAATT
TAAAAAGATTGATGCAAATATTAAATTTGTTGATTTAAATAATTTGCATTTTACTTTAAAATTCTTTGGAGATATAGATA
CTGAAGGTATAGATTTAATTTCTAAAAAAATTGAAAAAGTGGCTGATGATTTTGAACCATTTAATATTAAAATAAGTGGC
TGCGGAGCTTTTCCGAATAATAATCATATAAAGGTTATCTGGATTGGAGTTGAAAGTGACGAACTTTTAGTCCAGTTACA
TGACAAGCTAGATAATGAATTCGCAGCTATAGGTTTTGATAAGGATAAAAAATTCTCAACACATCTTACTATTGGCCGTA
TGAAATCAGCTAAAGGAAAAAATCAGGTTAGAGATATTATTGAAGAGTTTGAAAATGTTGAAATTGGGGAAATGAATATT
TCAAACATTGCTTTTAAGAAAAGCACTTTAAAGCCTTCAGGTCCGATTTATGAAAATTTAAAAACTTACGATTTGTGA

Upstream 100 bases:

>100_bases
TATCAAAATAGGGGATAAAGTTAAAGTATATGTTGAATCAAATGCCAGACATTTTGGTATAGCTATTGATGAAACAATAA
TTGAACAGTAAGGTGTTTTA

Downstream 100 bases:

>100_bases
TTTAATGGATTATAAACAGATTTTAAATGAGATTAAACCTTCAAAAGAGGAAAGCAGAGAAATTCTTAAGACTTCAGATA
AATTAATCAGTTATTTAAAT

Product: 2'-5' RNA ligase, LigT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSQIRSFLAIDLDDDFKPKVNKIIKEFKKIDANIKFVDLNNLHFTLKFFGDIDTEGIDLISKKIEKVADDFEPFNIKISG
CGAFPNNNHIKVIWIGVESDELLVQLHDKLDNEFAAIGFDKDKKFSTHLTIGRMKSAKGKNQVRDIIEEFENVEIGEMNI
SNIAFKKSTLKPSGPIYENLKTYDL

Sequences:

>Translated_185_residues
MSQIRSFLAIDLDDDFKPKVNKIIKEFKKIDANIKFVDLNNLHFTLKFFGDIDTEGIDLISKKIEKVADDFEPFNIKISG
CGAFPNNNHIKVIWIGVESDELLVQLHDKLDNEFAAIGFDKDKKFSTHLTIGRMKSAKGKNQVRDIIEEFENVEIGEMNI
SNIAFKKSTLKPSGPIYENLKTYDL
>Mature_184_residues
SQIRSFLAIDLDDDFKPKVNKIIKEFKKIDANIKFVDLNNLHFTLKFFGDIDTEGIDLISKKIEKVADDFEPFNIKISGC
GAFPNNNHIKVIWIGVESDELLVQLHDKLDNEFAAIGFDKDKKFSTHLTIGRMKSAKGKNQVRDIIEEFENVEIGEMNIS
NIAFKKSTLKPSGPIYENLKTYDL

Specific function: Unknown

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: NA

Molecular weight: Translated: 21142; Mature: 21011

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIRSFLAIDLDDDFKPKVNKIIKEFKKIDANIKFVDLNNLHFTLKFFGDIDTEGIDLI
CCHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCCHHHHH
SKKIEKVADDFEPFNIKISGCGAFPNNNHIKVIWIGVESDELLVQLHDKLDNEFAAIGFD
HHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEEECC
KDKKFSTHLTIGRMKSAKGKNQVRDIIEEFENVEIGEMNISNIAFKKSTLKPSGPIYENL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEECCEECCCEEEECCCCCCCCCHHHCC
KTYDL
EECCC
>Mature Secondary Structure 
SQIRSFLAIDLDDDFKPKVNKIIKEFKKIDANIKFVDLNNLHFTLKFFGDIDTEGIDLI
CHHHEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEECCEEEEEEEECCCCCCHHHHH
SKKIEKVADDFEPFNIKISGCGAFPNNNHIKVIWIGVESDELLVQLHDKLDNEFAAIGFD
HHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEEEEECCCHHHHHHHHHCCCCEEEEECC
KDKKFSTHLTIGRMKSAKGKNQVRDIIEEFENVEIGEMNISNIAFKKSTLKPSGPIYENL
CCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEECCEECCCEEEECCCCCCCCCHHHCC
KTYDL
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]