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The map label for this gene is cca [H]

Identifier: 148642113

GI number: 148642113

Start: 49123

End: 50493

Strand: Reverse

Name: cca [H]

Synonym: Msm_0053

Alternate gene names: 148642113

Gene position: 50493-49123 (Counterclockwise)

Preceding gene: 148642114

Following gene: 148642112

Centisome position: 2.72

GC content: 31.29

Gene sequence:

>1371_bases
ATGGATTATAAACAGATTTTAAATGAGATTAAACCTTCAAAAGAGGAAAGCAGAGAAATTCTTAAGACTTCAGATAAATT
AATCAGTTATTTAAATGAAGTCTGCAAAGATGAAGGCATCACTGCTAGTGCTTCACTTGTAGGTTCTGTAGCTAAGAAAA
CTTATTTAAGCGGAAAATCAGATATTGATATCTTTATTTCCTTTCCTTTAGATGTGGATGTTGCACAGCTTAAAAAAATA
GGTTTATATTTGGGATATAAATGCAGCGATTATTTTAATGGAGATGCCAGTGAACATTATGCATCTCACCCATATGTAAC
TAGTCAGATTAATGGTTTTGAAGTGGATTTTGTTCCATGCTATAAAATTGAAGACGGAAGCCAGCTGAAATCTGCAGTTG
ACAGGACAATTTTACATACCAAATATATTAAATCTCATTTAGAACAAGACCAGAATGAAGAAGTTCTTCTTTTAAAAAGA
TTCATGGACATGACAGGAACATATGGCTCTGAATTTAAAGTAGGGGGCTTTGCAGGTTATCTGTGTGAACTGCTGATTTT
AAAATATGGGACCTTTGAAAACACTTTAAAGGAAGCTTCAAAATGGAGATATGGTCATACTGTTGATTTGGAAGGTTATG
GTACTGCAGCTTTGTTTAATGACCCGTTAATCGTAATAGATCCCACTGATATGAACCGTAATGTTGGGGCTGCATTAAGA
TTAGATAAAATGGCTGAATTTATCCAATCTGCAAGAAATTATTTAGCCAGCAACAATAAAAAAGAGTATTTTTACCCGGT
AGCTAATAAATTTGCTAAAGATGAAATTTTAGAAGAATTTCAAAAAAGAGGCAGTGATGTAATAGCTGTTAAATTTAATA
TTCCTGACATTCCTTTGGATACCCTTCATCCTCAGCTTAGAAAAACAACCGAGTCTTTAGCTGAGAAATTAAATGATGAA
GATTTTCCTGTTTACCAATCTGATTACTGGAGTGATGAGGAAAATACAGCTGTTTTGCTTTTTGAGATGACTGTGTCCAG
ATTAAATAATACAAAAATCCATTATGGTCCGAAGGTTTTTGTTAAAAGAGGCTCACAAAACTTTATTGACAAGCATGGAA
TTGAAAACTGCTATATTTTAGATGATTTTGTTGTTTCTAAAATCTCAAGGGAATTTGTAACAGCTTCTGATTTTATTAAT
TATGTTTTAAGTTATGAAAATATTTCCTTAATAAAAGTAGGTAAAAATCTTTTGGATAATATAATTACCAGCTATGAACT
CCTTTCATTAGATGATTTGGATTTAAGTAATGAAAGTTTCGTAACTTTTTTAGATGATTTTTTAAATCCAAATAAATACT
TATTTAGATAA

Upstream 100 bases:

>100_bases
ATTGGGGAAATGAATATTTCAAACATTGCTTTTAAGAAAAGCACTTTAAAGCCTTCAGGTCCGATTTATGAAAATTTAAA
AACTTACGATTTGTGATTTA

Downstream 100 bases:

>100_bases
TTCTTTTTTTTAATTTTGGATTGTCCTTAAATTTCATTGCTATAATTCAATTCAATTAGAAATGATTATTAGTAAATAAA
ATATAATTAATTAATGTTAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDYKQILNEIKPSKEESREILKTSDKLISYLNEVCKDEGITASASLVGSVAKKTYLSGKSDIDIFISFPLDVDVAQLKKI
GLYLGYKCSDYFNGDASEHYASHPYVTSQINGFEVDFVPCYKIEDGSQLKSAVDRTILHTKYIKSHLEQDQNEEVLLLKR
FMDMTGTYGSEFKVGGFAGYLCELLILKYGTFENTLKEASKWRYGHTVDLEGYGTAALFNDPLIVIDPTDMNRNVGAALR
LDKMAEFIQSARNYLASNNKKEYFYPVANKFAKDEILEEFQKRGSDVIAVKFNIPDIPLDTLHPQLRKTTESLAEKLNDE
DFPVYQSDYWSDEENTAVLLFEMTVSRLNNTKIHYGPKVFVKRGSQNFIDKHGIENCYILDDFVVSKISREFVTASDFIN
YVLSYENISLIKVGKNLLDNIITSYELLSLDDLDLSNESFVTFLDDFLNPNKYLFR

Sequences:

>Translated_456_residues
MDYKQILNEIKPSKEESREILKTSDKLISYLNEVCKDEGITASASLVGSVAKKTYLSGKSDIDIFISFPLDVDVAQLKKI
GLYLGYKCSDYFNGDASEHYASHPYVTSQINGFEVDFVPCYKIEDGSQLKSAVDRTILHTKYIKSHLEQDQNEEVLLLKR
FMDMTGTYGSEFKVGGFAGYLCELLILKYGTFENTLKEASKWRYGHTVDLEGYGTAALFNDPLIVIDPTDMNRNVGAALR
LDKMAEFIQSARNYLASNNKKEYFYPVANKFAKDEILEEFQKRGSDVIAVKFNIPDIPLDTLHPQLRKTTESLAEKLNDE
DFPVYQSDYWSDEENTAVLLFEMTVSRLNNTKIHYGPKVFVKRGSQNFIDKHGIENCYILDDFVVSKISREFVTASDFIN
YVLSYENISLIKVGKNLLDNIITSYELLSLDDLDLSNESFVTFLDDFLNPNKYLFR
>Mature_456_residues
MDYKQILNEIKPSKEESREILKTSDKLISYLNEVCKDEGITASASLVGSVAKKTYLSGKSDIDIFISFPLDVDVAQLKKI
GLYLGYKCSDYFNGDASEHYASHPYVTSQINGFEVDFVPCYKIEDGSQLKSAVDRTILHTKYIKSHLEQDQNEEVLLLKR
FMDMTGTYGSEFKVGGFAGYLCELLILKYGTFENTLKEASKWRYGHTVDLEGYGTAALFNDPLIVIDPTDMNRNVGAALR
LDKMAEFIQSARNYLASNNKKEYFYPVANKFAKDEILEEFQKRGSDVIAVKFNIPDIPLDTLHPQLRKTTESLAEKLNDE
DFPVYQSDYWSDEENTAVLLFEMTVSRLNNTKIHYGPKVFVKRGSQNFIDKHGIENCYILDDFVVSKISREFVTASDFIN
YVLSYENISLIKVGKNLLDNIITSYELLSLDDLDLSNESFVTFLDDFLNPNKYLFR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329 [H]

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2 [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 52096; Mature: 52096

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYKQILNEIKPSKEESREILKTSDKLISYLNEVCKDEGITASASLVGSVAKKTYLSGKS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCC
DIDIFISFPLDVDVAQLKKIGLYLGYKCSDYFNGDASEHYASHPYVTSQINGFEVDFVPC
CEEEEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCHHHCCCCCEEEECCCCEEEEEEEE
YKIEDGSQLKSAVDRTILHTKYIKSHLEQDQNEEVLLLKRFMDMTGTYGSEFKVGGFAGY
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCHHHHH
LCELLILKYGTFENTLKEASKWRYGHTVDLEGYGTAALFNDPLIVIDPTDMNRNVGAALR
HHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEECCCCCCCCCCHHHH
LDKMAEFIQSARNYLASNNKKEYFYPVANKFAKDEILEEFQKRGSDVIAVKFNIPDIPLD
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
TLHPQLRKTTESLAEKLNDEDFPVYQSDYWSDEENTAVLLFEMTVSRLNNTKIHYGPKVF
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCEEEECCEEE
VKRGSQNFIDKHGIENCYILDDFVVSKISREFVTASDFINYVLSYENISLIKVGKNLLDN
EECCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
IITSYELLSLDDLDLSNESFVTFLDDFLNPNKYLFR
HHHHHHEEECCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDYKQILNEIKPSKEESREILKTSDKLISYLNEVCKDEGITASASLVGSVAKKTYLSGKS
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCC
DIDIFISFPLDVDVAQLKKIGLYLGYKCSDYFNGDASEHYASHPYVTSQINGFEVDFVPC
CEEEEEECCCCCCHHHHHHHHHHCCCEECCCCCCCCHHHCCCCCEEEECCCCEEEEEEEE
YKIEDGSQLKSAVDRTILHTKYIKSHLEQDQNEEVLLLKRFMDMTGTYGSEFKVGGFAGY
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEECCHHHHH
LCELLILKYGTFENTLKEASKWRYGHTVDLEGYGTAALFNDPLIVIDPTDMNRNVGAALR
HHHHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEECCCCCCCCCCHHHH
LDKMAEFIQSARNYLASNNKKEYFYPVANKFAKDEILEEFQKRGSDVIAVKFNIPDIPLD
HHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
TLHPQLRKTTESLAEKLNDEDFPVYQSDYWSDEENTAVLLFEMTVSRLNNTKIHYGPKVF
HCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCEEEECCEEE
VKRGSQNFIDKHGIENCYILDDFVVSKISREFVTASDFINYVLSYENISLIKVGKNLLDN
EECCCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHH
IITSYELLSLDDLDLSNESFVTFLDDFLNPNKYLFR
HHHHHHEEECCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]