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The map label for this gene is thiDE [H]

Identifier: 148359104

GI number: 148359104

Start: 1801654

End: 1803141

Strand: Direct

Name: thiDE [H]

Synonym: LPC_0992

Alternate gene names: 148359104

Gene position: 1801654-1803141 (Clockwise)

Preceding gene: 148359103

Following gene: 148359105

Centisome position: 50.38

GC content: 39.25

Gene sequence:

>1488_bases
ATGAAAAAACCGATTGTTTGGACAATTGCTGGTGTTGACTCATCTGGATTAGCTGGTGTCCATGCTGACATGGAAACTTT
TAGTCGCTTGAATGTCAGGGCTTGTTCAGTCATCACCGCAGTAACAGCACAAAATGCTCACTCAATTACAGCAGTAGAAG
CGATATCCAGAGATCAGGTTGCTGCTCAGTGCCGTGCCTTGGAATTAAATTTAAAACCGGATGCCATTAAAATTGGTATG
TTATGCTCTACACCAATCTGTGAAGAAATAGCTTATTTTTTAAAAGGTTATGAAGGATTTGTTGTTCTTGATCCGATAAT
TACCTCCTCCTCGGGAACTAACCTGTTTTTTCCAGATTTACAACAGCATAAAGAAAATCTAATTCAATTGTTTCCGTACG
TTACTATTATTACTCCTAATCGGATTGAAGCAGAAATTATTTTGAATCGGAGCATTTCTTCTTATCAGGATATAATCAAT
GCTGCTTCAGATCTTTTATCACTGGGAGCAAAGCAGGTGTTACTGAAAGGAGGACATGTAAAAGATAACTCATTCAGCCA
AGATTACTGGACTGATGGAAAAGAGTTATTTTGGATTGCAAATCGTCGTTTTCCTGAAACAAATTACCGTGGGACAGGCT
GTGTTCTATCTTCAGCACTCACAGCTTGTCTTGCGCTAGGCTATTCAATAAAAGATGCTATTGTTATTGCTAAAATGTAT
GTCAATCGCGGCATACGCCAATCAATAGAACTTGATAAAGACGCCTCGCAACTTTATCACGATGGTTGGCCAGAAGATGA
GGCCGATCTCCCCTATTTATCACCAACACCGTTCATTAAACCAGTTCCCTCATTTAAAAAATGTTCTATGGGTTTTTATC
CCATTGTGGATAGTAGCCACTGGTTAGAAATGCTTTTGCCATTGGGAATTAAGTGCATTCAGTTACGAATAAAGGAAGCC
TCTCAAGAGCGGTTGGAAGAAGAGATAAAACGGAGTGTCCATTTGGCAAATCAGTATAATGCTGCATTATTTATTAATGA
TCACTGGGAACTTGCTATTCATTATGGTGCTGCAGGAGTTCACCTTGGCCAAGAGGATCTGGAAAAAGCTGATGTCGATA
GAATTAATCGAGCAGGTTTATTCTTAGGCATAAGTACGCATTGCTATTACGAAGTTGCTCGGGCTCATGCTCTTAACCCT
TCCTACGTCGCTTGCGGCCCCATATATGAAACAACCAGCAAAATAATGCCATTTCAAGCACAAGGAATTGCACGACTTGA
ACGTTGGCGAAAAACGCTTCGTTATCCATTGGTTGCCATTGGAGGTATAACTTTAAAGAACCTTTCAGATGTCTTAAAAA
CAAAAATTGATGGTGTGTCAGTGATCTCTGCCATTACAAAAGCATCAGTACCTTTAGTTGCAGCCAAACAATTTTTAACT
CAAATGAATGAATCACATAATGAACAAATCAAATTTGGCAATGAATGA

Upstream 100 bases:

>100_bases
GTCATCAGGCATTTATAGGAGGAATGATGTCTGAGAGAAATGTAGCTCACCCAAGTACTCCATTAATCGATACTCCATTT
TGGCATCAGGTAAACAATTT

Downstream 100 bases:

>100_bases
ATTAACCCGCTATTCTCAACAAATAAAGATGGAAGAAATTGGATTAGACGGACAGGAGAAACTTAAGAATTCTCGAGTAT
TATGCATTGGGCTTGGCGGT

Product: bifunctional phosphomethylpyrimidine kinase ThiD/thiamin- phosphate pyrophosphorylase ThiE

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQVAAQCRALELNLKPDAIKIGM
LCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKENLIQLFPYVTIITPNRIEAEIILNRSISSYQDIIN
AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKELFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDAIVIAKMY
VNRGIRQSIELDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKCSMGFYPIVDSSHWLEMLLPLGIKCIQLRIKEA
SQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNP
SYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASVPLVAAKQFLT
QMNESHNEQIKFGNE

Sequences:

>Translated_495_residues
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQVAAQCRALELNLKPDAIKIGM
LCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKENLIQLFPYVTIITPNRIEAEIILNRSISSYQDIIN
AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKELFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDAIVIAKMY
VNRGIRQSIELDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKCSMGFYPIVDSSHWLEMLLPLGIKCIQLRIKEA
SQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNP
SYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASVPLVAAKQFLT
QMNESHNEQIKFGNE
>Mature_495_residues
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQVAAQCRALELNLKPDAIKIGM
LCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDLQQHKENLIQLFPYVTIITPNRIEAEIILNRSISSYQDIIN
AASDLLSLGAKQVLLKGGHVKDNSFSQDYWTDGKELFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDAIVIAKMY
VNRGIRQSIELDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKCSMGFYPIVDSSHWLEMLLPLGIKCIQLRIKEA
SQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGVHLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNP
SYVACGPIYETTSKIMPFQAQGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASVPLVAAKQFLT
QMNESHNEQIKFGNE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=204, Percent_Identity=45.5882352941176, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1788420, Length=254, Percent_Identity=36.6141732283465, Blast_Score=138, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6325378, Length=272, Percent_Identity=29.4117647058824, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324997, Length=270, Percent_Identity=31.1111111111111, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324517, Length=273, Percent_Identity=31.1355311355311, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 54982; Mature: 54982

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDL
HHHHHEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCEEECCCCCEEECCCH
QQHKENLIQLFPYVTIITPNRIEAEIILNRSISSYQDIINAASDLLSLGAKQVLLKGGHV
HHHHHHHHHHCCEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHHCCCCC
KDNSFSQDYWTDGKELFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDAIVIAKMY
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VNRGIRQSIELDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKCSMGFYPIVDSSH
HCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCHH
WLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGV
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCC
HLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNPSYVACGPIYETTSKIMPFQA
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCH
QGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASVPLVAAKQFLT
HHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHH
QMNESHNEQIKFGNE
HHCCCCCCCCCCCCC
>Mature Secondary Structure
MKKPIVWTIAGVDSSGLAGVHADMETFSRLNVRACSVITAVTAQNAHSITAVEAISRDQV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AAQCRALELNLKPDAIKIGMLCSTPICEEIAYFLKGYEGFVVLDPIITSSSGTNLFFPDL
HHHHHEEEEECCCCEEEEEEEECCHHHHHHHHHHCCCCCEEEECCEEECCCCCEEECCCH
QQHKENLIQLFPYVTIITPNRIEAEIILNRSISSYQDIINAASDLLSLGAKQVLLKGGHV
HHHHHHHHHHCCEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCHHHHHHCCCCC
KDNSFSQDYWTDGKELFWIANRRFPETNYRGTGCVLSSALTACLALGYSIKDAIVIAKMY
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VNRGIRQSIELDKDASQLYHDGWPEDEADLPYLSPTPFIKPVPSFKKCSMGFYPIVDSSH
HCCCHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCHH
WLEMLLPLGIKCIQLRIKEASQERLEEEIKRSVHLANQYNAALFINDHWELAIHYGAAGV
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCC
HLGQEDLEKADVDRINRAGLFLGISTHCYYEVARAHALNPSYVACGPIYETTSKIMPFQA
CCCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCCCH
QGIARLERWRKTLRYPLVAIGGITLKNLSDVLKTKIDGVSVISAITKASVPLVAAKQFLT
HHHHHHHHHHHHHHCCEEEECCEEHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHH
QMNESHNEQIKFGNE
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]