Click here to switch to the map view.

The map label for this gene is thiG [H]

Identifier: 148359103

GI number: 148359103

Start: 1800869

End: 1801657

Strand: Direct

Name: thiG [H]

Synonym: LPC_0991

Alternate gene names: 148359103

Gene position: 1800869-1801657 (Clockwise)

Preceding gene: 148359102

Following gene: 148359104

Centisome position: 50.35

GC content: 41.95

Gene sequence:

>789_bases
ATGTGGAATTTGGCAGGAAAAGAATTAAGTAGCCGTTTGCTCTTAGGCACTGCGTGTTATCCTTCATTAGAGCATATGCA
GCAGGCAATTCATAATTCGGGTACAGAAGTTATTACGATATCAATAAAACGCCAGACTTCTGCAGGATTGGATGGAGAGT
CCTTTTGGCAAGCGGTAAAAAAACTAGATTGTCATCTTTTGCCCAATACAGCTGGTTGCCGTAATGCGGAGGCAGCGATC
AATACAGCTGAAATTGCGAGGGAGTTATTTAATACTCACTGGATAAAACTGGAAGTGATAGGCGATGATTATAATTTACA
GCCCGAACCATTTGAGTTAATCAAGGCAGCCAGAATTTTGATTGACAGAGGATTTGAAGTTTTTCCTTATTGCACAGATG
ATCTCGTTTTATGTCAAAAACTGGTTGATGCTGGTTGTAAAATTTTAATGCCCTGGGGAGCTCCTATAGGCTCAGGTAAG
GGTTTAATTAACCCCTATGCCTTGGAAACTCTGCGTTATCGATTTCCTGACATCACATTAATCATTGATGCCGGGATTGG
TAAGCCATCTCATGCTGTACAGGTGATGGAATTGGGTTTTGATGGCGTGCTTCTAAATACTGCTGTAGCTCTTGCCAATC
ATCCTGCTCTCATGGCAACCGCATTCCGCCATGCCGTTATTGCAGGTCATCAGGCATTTATAGGAGGAATGATGTCTGAG
AGAAATGTAGCTCACCCAAGTACTCCATTAATCGATACTCCATTTTGGCATCAGGTAAACAATTTATGA

Upstream 100 bases:

>100_bases
CTATAAATAATCATCATGTACCACATAAAGCTTATGAAACTACAATTTTGCAAGCCAATGACTGCATTGATATCCTCTTT
CCCATGCAAGGAGGTTAATG

Downstream 100 bases:

>100_bases
AAAAACCGATTGTTTGGACAATTGCTGGTGTTGACTCATCTGGATTAGCTGGTGTCCATGCTGACATGGAAACTTTTAGT
CGCTTGAATGTCAGGGCTTG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MWNLAGKELSSRLLLGTACYPSLEHMQQAIHNSGTEVITISIKRQTSAGLDGESFWQAVKKLDCHLLPNTAGCRNAEAAI
NTAEIARELFNTHWIKLEVIGDDYNLQPEPFELIKAARILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALMATAFRHAVIAGHQAFIGGMMSE
RNVAHPSTPLIDTPFWHQVNNL

Sequences:

>Translated_262_residues
MWNLAGKELSSRLLLGTACYPSLEHMQQAIHNSGTEVITISIKRQTSAGLDGESFWQAVKKLDCHLLPNTAGCRNAEAAI
NTAEIARELFNTHWIKLEVIGDDYNLQPEPFELIKAARILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALMATAFRHAVIAGHQAFIGGMMSE
RNVAHPSTPLIDTPFWHQVNNL
>Mature_262_residues
MWNLAGKELSSRLLLGTACYPSLEHMQQAIHNSGTEVITISIKRQTSAGLDGESFWQAVKKLDCHLLPNTAGCRNAEAAI
NTAEIARELFNTHWIKLEVIGDDYNLQPEPFELIKAARILIDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGK
GLINPYALETLRYRFPDITLIIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALMATAFRHAVIAGHQAFIGGMMSE
RNVAHPSTPLIDTPFWHQVNNL

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=250, Percent_Identity=42.4, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28739; Mature: 28739

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNLAGKELSSRLLLGTACYPSLEHMQQAIHNSGTEVITISIKRQTSAGLDGESFWQAVK
CCCCCCHHHHHHEEEEHHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHH
KLDCHLLPNTAGCRNAEAAINTAEIARELFNTHWIKLEVIGDDYNLQPEPFELIKAARIL
HCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
IDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGKGLINPYALETLRYRFPDITL
HHCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEE
IIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALMATAFRHAVIAGHQAFIGGMMSE
EEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCC
RNVAHPSTPLIDTPFWHQVNNL
CCCCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MWNLAGKELSSRLLLGTACYPSLEHMQQAIHNSGTEVITISIKRQTSAGLDGESFWQAVK
CCCCCCHHHHHHEEEEHHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHH
KLDCHLLPNTAGCRNAEAAINTAEIARELFNTHWIKLEVIGDDYNLQPEPFELIKAARIL
HCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHH
IDRGFEVFPYCTDDLVLCQKLVDAGCKILMPWGAPIGSGKGLINPYALETLRYRFPDITL
HHCCCEEECCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEE
IIDAGIGKPSHAVQVMELGFDGVLLNTAVALANHPALMATAFRHAVIAGHQAFIGGMMSE
EEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHCC
RNVAHPSTPLIDTPFWHQVNNL
CCCCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA