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The map label for this gene is lspE [H]

Identifier: 148358851

GI number: 148358851

Start: 1519114

End: 1520598

Strand: Reverse

Name: lspE [H]

Synonym: LPC_0734

Alternate gene names: 148358851

Gene position: 1520598-1519114 (Counterclockwise)

Preceding gene: 148358852

Following gene: 148358843

Centisome position: 42.52

GC content: 38.99

Gene sequence:

>1485_bases
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACAGTTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATACAAGA
AAATCTGGCTATTGTCTATCATTTACCTAGCACACCACTGCAAGCTTTCGCTGAGATAAAGCGATTATTGCAATGCGAAC
TTGATCTAAAACAAGTCGATGAATCCACTTTTCAGCAGCATTTAGCCAATATTTACCAATCTAAATCTTCAATACTGGAT
GCAGCAGAAGGCATGGAAGAAGATATGGATTTATCCATGTTGGCCAGTCAACTTCCAGTCAGTGAAGATTTATTGGAAAA
TCAAGACGATGCTCCAATTATCCGTTTACTGAATGCTCTGTTCACTCAAGCCATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTGGTCCGCAATCGAATTGATGGTGTTCTACAAGAAGTCCTTGAAATTCAACGAGCT
ATCGCTCCTTTGGTCATTTCCAGAGTTAAGGTGATGGCCAAATTGGATATCGCCGAAAAGAGAATTCCTCAAGATGGTCG
AATTTCATTGCGTATAGGAGGACACAATATTGATGTCAGGGTCTCAACTTTACCCTCAAACCATGGAGAACGAGTAGTTT
TAAGGATATTAGATAAACAAGCTGCTCAACTGGATTTAAATCTTTTGGGTATGCCTGAACATACATTAAAAACAGTACGA
CAAATGATTGCTGAACCCCATGGCATTATTTTAGTCACTGGCCCAACAGGATCAGGCAAGACAACTTCTCTCTATGCTAT
GCTCACCGAACTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGACCTGCCTGGTGTAGGAC
AAACGCAGGTTAATACTAAAGTTCAAATGACTTTTGCCAAAGGATTAAGGGCCATATTACGTCAAGACCCGGATGTTGTC
ATGATAGGAGAAATCCGTGATTTGGAAACGGCTGAAATTGCTGTTCAAGCAAGCTTAACAGGGCATTTGGTGCTATCAAC
CCTTCATACCAATACCGCATTGGGAGCATTAACTCGCCTGCATGATATGGGAGTTGAGTCTTTTTTATTGTCATCCAGTA
TAGTCGGCCTCATTGCGCAACGTTTGGTAAGGAAATTATGCTCTCATTGCAAAACACCTCATGAATTAAGACATGAAGAA
AAGGAGTTAATGGGTCTTAAACCTGATGCTGATGTATCTCAGGTCTTTGAACCCAAGGGATGCGACTATTGCAACCACTT
GGGTTATAAAGGCAGAACAGGAATTTATGAATTAATTATCGTAGATGAAACCCTGAGAGGAATGATTCATCGTAATGAAA
ATATTCAAACAATGGAAAATTATTTACGACCAACCACACCAAGCATACGTGAAGATGGTTTTAAAAGAGTATTATGTGGG
GACACCTCACTTGCAGAAATTTTAAGGGTAACATCCCAAAACTAA

Upstream 100 bases:

>100_bases
AACAGATGATGAAACAGTGTTGCCCCCATTAACTCAAGGTAATCTACCAGTGCCATTTAGCGCTCCCTCCCAATATCCAT
CAAAAATGACCAAATAAATC

Downstream 100 bases:

>100_bases
TTATTTTCCCAGTACAAAGACTAACGTTTTAATATCTTTTGATTACCAATTTATTTTTAAAGACAATCTTTTATTTGCTA
TAATACTTGCGCATCAAATC

Product: type II protein secretion ATPase LspE

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPSTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRHEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Sequences:

>Translated_494_residues
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPSTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRHEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN
>Mature_494_residues
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPSTPLQAFAEIKRLLQCELDLKQVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRHEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=484, Percent_Identity=52.4793388429752, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1786296, Length=382, Percent_Identity=42.6701570680628, Blast_Score=311, Evalue=5e-86,
Organism=Escherichia coli, GI87082188, Length=255, Percent_Identity=36.4705882352941, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55134; Mature: 55134

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPSTPLQAFAEIKRLLQCELDLKQVD
CCCCCCCEECCCHHHCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
ESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
FTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRAIAPLVISRVKVMAKLDIAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
HCCCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHH
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTK
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCE
VQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRL
EEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCEEHHHHHHHHHHHHHHHHH
HDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRHEEKELMGLKPDADVSQVFEPKG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCC
CDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
CCHHHHCCCCCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCEEEEC
DTSLAEILRVTSQN
CHHHHHHHHHHCCC
>Mature Secondary Structure
MENEEKSLKIPYSFAKSNGIVVGEIQENLAIVYHLPSTPLQAFAEIKRLLQCELDLKQVD
CCCCCCCEECCCHHHCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHHHH
ESTFQQHLANIYQSKSSILDAAEGMEEDMDLSMLASQLPVSEDLLENQDDAPIIRLLNAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHCCCCCCHHHHHHHHH
FTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRAIAPLVISRVKVMAKLDIAEK
HHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPEHTLKTVR
HCCCCCEEEEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHH
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGVGQTQVNTK
HHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCE
VQMTFAKGLRAILRQDPDVVMIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRL
EEHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCEEHHHHHHHHHHHHHHHHH
HDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHELRHEEKELMGLKPDADVSQVFEPKG
HHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCC
CDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENIQTMENYLRPTTPSIREDGFKRVLCG
CCHHHHCCCCCCCCEEEEEEECHHHHHHHHCCCCHHHHHHHCCCCCCCHHHCCCCEEEEC
DTSLAEILRVTSQN
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]