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The map label for this gene is traG [H]

Identifier: 148358319

GI number: 148358319

Start: 195680

End: 197569

Strand: Direct

Name: traG [H]

Synonym: LPC_0181

Alternate gene names: 148358319

Gene position: 195680-197569 (Clockwise)

Preceding gene: 148358318

Following gene: 148358320

Centisome position: 5.47

GC content: 42.28

Gene sequence:

>1890_bases
GTGAGCATGAGCAAACCCAATAAATCCATTGGCCCACAGGTAAGAAAAAAGCGCGTTGAAAAGACGAATAAACAAATCGT
TTTTTTAGTTGTGCTCGCGCTTTTCGTCAGTATGCAGATTGGTACGCAATTCGTGGCTTATAAACTTCATTACCACCAAA
GCTTAGGATTTTCAGTGGCGCATATTTATCTGCCATGGCAGCTACTTTGGTGGAATCTGCAATACCATTCTATTTACCCG
GATTATTTTAACGCGGCCTTTGGCCTGGTGGCGATGAGTGGCAGTCTTTTTTTAATGATCATTCTTTTTGTCAGTAAGCA
ACTTACTCAAGAAAACGTCAGTGAATATCTGCATGGTTCAGCCCGATGGGCTAATCAAGATGATTTAAAAGATGCAGGTT
TGATTGGCAACGAGGAAGGCGTTTATGTGGGTGCCGTTGAAAATGAAAAAGGTGATATTCATTATTTACGCCATAATGGG
CCTGAACATATTTTAACTTATGCGCCAACGCGCTCCGGTAAAGGTGTAGGACTTGTTATTCCTACTCTTTTATCCTGGAA
ACAGTCTTGTGTGATTACCGATTTAAAAGGCGAACTTTGGGCATTAACTGCTGGCTGGCGGCAAAAACATGCCAACAATA
AAGTAATACGTTTTGAGCCAGCGACATTAAAAGGTTCAGCGCGTTGGAATCCGTTGGATGAAATCAGAGTCGGTACGGAA
TATGAAGTCGGTGATGTGCAAAATCTTGCAACGCTTGTGGTTGATCCAGACGGTAAAGGACTCGAAACCCATTGGCAAAA
AACATCACAAGCGCTTTTAGTCGGTTTTATCCTCCATGCAATTTACAAGCTAAAAAATCAGGGAGAGCCAGCCACATTTC
CCAACATTGACAGGATGCTGGTAGATCCCAATACCAATATTGCCGATTTATTGATTGAAATGACCCAATACCCACATATT
GATGGCAAAACACATCCTGTGATCAGCGCATCAGCCCGCGATATGATCGACAGGCCCGAAGAAGAAGCAGGCTCTGTATT
ATCGACTTTAAAATCGTATCTGGCCTTATACCGAGATCCAGTCGTGGCGCACAATGTTTCTGCCTCTGATTTCTGTATTA
AGGATTTGATGCATCATAATAATCCTGTGAGTCTTTATATCGTCACCCAACCCAATGATAAAGCACGATTGCAACCATTG
GTTCGCGTCATGCTGAATATGGTTGTCCGGCTTTTGGCAGATAGAATGGAATTTGAGCGAGTTGATGATGGGAAAGGAAA
CCGATTTGTAAGAACGAAAAGAAACTATAAGCACCGTTTGCTGTGCATGATTGATGAATTTCCAAGTCTTGGCAAACTGG
ATATTTTGCAAGAGTCCTTAGCATTTGTTGCAGGTTATGGTTTAAAGTTTTATTTAATCTGTCAGGACATTAACCAGCTT
AAAAGCCGCGAAAGAGGCTATGGGCCGGATGAAACGATCACTTCTAACTGCCATATTCAAAATGCTTATCCTCCCAACCG
GATTGAAACGGCTGAACATCTATCGAAATTAACCGGCCAAACCACTATTGTCAAAGAGCACATCACCACCAGCGGCAAGC
GTGTTTCAACCTTTTTAAATCAGATTTCTAAAACAAAACAAGAAGTTTCTAGGCCGCTTTTAACCATTGACGAATGTCAG
CGTATGCCAGGGCCTAAAAAGGATGCTAATGGCTTAATTACTGAAGCGGGTGATATGGTGGTGTATGTTGCAGGTTTTCC
CGCTATATATGGAAAACAACCGCTTTATTTCAAAGATCCAGTGTTTATCGCCAGAGCTTCCGTTGAGTCACCTGAGCAAT
CCGATATCTTACGTACTCGTCTTTCTGAAGATGAGGAAATCAGGCTATGA

Upstream 100 bases:

>100_bases
AAGGCAGTTTCAGTTCGGGAAGTGGCGAATATAATCAAGAATCAGGCGGTGCAGATGAAGTCAGTCAGTTTGTCAATCAA
AAAGCATCTGGAGAAAGCCT

Downstream 100 bases:

>100_bases
AAAAGTTTATGGCCTGGGTTGCTGTTGTTCTTTTAGGCCTTATCAGCCTATCGTTTCTTTTAATTATTATGGGCTTTCGA
GTCAACATTACGGATTCGAT

Product: conjugal transfer coupling protein TraG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSMSKPNKSIGPQVRKKRVEKTNKQIVFLVVLALFVSMQIGTQFVAYKLHYHQSLGFSVAHIYLPWQLLWWNLQYHSIYP
DYFNAAFGLVAMSGSLFLMIILFVSKQLTQENVSEYLHGSARWANQDDLKDAGLIGNEEGVYVGAVENEKGDIHYLRHNG
PEHILTYAPTRSGKGVGLVIPTLLSWKQSCVITDLKGELWALTAGWRQKHANNKVIRFEPATLKGSARWNPLDEIRVGTE
YEVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGFILHAIYKLKNQGEPATFPNIDRMLVDPNTNIADLLIEMTQYPHI
DGKTHPVISASARDMIDRPEEEAGSVLSTLKSYLALYRDPVVAHNVSASDFCIKDLMHHNNPVSLYIVTQPNDKARLQPL
VRVMLNMVVRLLADRMEFERVDDGKGNRFVRTKRNYKHRLLCMIDEFPSLGKLDILQESLAFVAGYGLKFYLICQDINQL
KSRERGYGPDETITSNCHIQNAYPPNRIETAEHLSKLTGQTTIVKEHITTSGKRVSTFLNQISKTKQEVSRPLLTIDECQ
RMPGPKKDANGLITEAGDMVVYVAGFPAIYGKQPLYFKDPVFIARASVESPEQSDILRTRLSEDEEIRL

Sequences:

>Translated_629_residues
MSMSKPNKSIGPQVRKKRVEKTNKQIVFLVVLALFVSMQIGTQFVAYKLHYHQSLGFSVAHIYLPWQLLWWNLQYHSIYP
DYFNAAFGLVAMSGSLFLMIILFVSKQLTQENVSEYLHGSARWANQDDLKDAGLIGNEEGVYVGAVENEKGDIHYLRHNG
PEHILTYAPTRSGKGVGLVIPTLLSWKQSCVITDLKGELWALTAGWRQKHANNKVIRFEPATLKGSARWNPLDEIRVGTE
YEVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGFILHAIYKLKNQGEPATFPNIDRMLVDPNTNIADLLIEMTQYPHI
DGKTHPVISASARDMIDRPEEEAGSVLSTLKSYLALYRDPVVAHNVSASDFCIKDLMHHNNPVSLYIVTQPNDKARLQPL
VRVMLNMVVRLLADRMEFERVDDGKGNRFVRTKRNYKHRLLCMIDEFPSLGKLDILQESLAFVAGYGLKFYLICQDINQL
KSRERGYGPDETITSNCHIQNAYPPNRIETAEHLSKLTGQTTIVKEHITTSGKRVSTFLNQISKTKQEVSRPLLTIDECQ
RMPGPKKDANGLITEAGDMVVYVAGFPAIYGKQPLYFKDPVFIARASVESPEQSDILRTRLSEDEEIRL
>Mature_628_residues
SMSKPNKSIGPQVRKKRVEKTNKQIVFLVVLALFVSMQIGTQFVAYKLHYHQSLGFSVAHIYLPWQLLWWNLQYHSIYPD
YFNAAFGLVAMSGSLFLMIILFVSKQLTQENVSEYLHGSARWANQDDLKDAGLIGNEEGVYVGAVENEKGDIHYLRHNGP
EHILTYAPTRSGKGVGLVIPTLLSWKQSCVITDLKGELWALTAGWRQKHANNKVIRFEPATLKGSARWNPLDEIRVGTEY
EVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGFILHAIYKLKNQGEPATFPNIDRMLVDPNTNIADLLIEMTQYPHID
GKTHPVISASARDMIDRPEEEAGSVLSTLKSYLALYRDPVVAHNVSASDFCIKDLMHHNNPVSLYIVTQPNDKARLQPLV
RVMLNMVVRLLADRMEFERVDDGKGNRFVRTKRNYKHRLLCMIDEFPSLGKLDILQESLAFVAGYGLKFYLICQDINQLK
SRERGYGPDETITSNCHIQNAYPPNRIETAEHLSKLTGQTTIVKEHITTSGKRVSTFLNQISKTKQEVSRPLLTIDECQR
MPGPKKDANGLITEAGDMVVYVAGFPAIYGKQPLYFKDPVFIARASVESPEQSDILRTRLSEDEEIRL

Specific function: Required for conjugative transfer of plasmid RP4. Binds tightly and specifically to the relaxase traI. Can also bind to DNA without sequence specificity. May form a pore-like structure that could serve as a channel for DNA transfer [H]

COG id: COG3505

COG function: function code U; Type IV secretory pathway, VirD4 components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virD4/traG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003688 [H]

Pfam domain/function: PF02534 TraG [H]

EC number: NA

Molecular weight: Translated: 71091; Mature: 70959

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSKPNKSIGPQVRKKRVEKTNKQIVFLVVLALFVSMQIGTQFVAYKLHYHQSLGFSVA
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCEEE
HIYLPWQLLWWNLQYHSIYPDYFNAAFGLVAMSGSLFLMIILFVSKQLTQENVSEYLHGS
EEEECEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARWANQDDLKDAGLIGNEEGVYVGAVENEKGDIHYLRHNGPEHILTYAPTRSGKGVGLVI
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCEEEECCCCCCCCCEEHH
PTLLSWKQSCVITDLKGELWALTAGWRQKHANNKVIRFEPATLKGSARWNPLDEIRVGTE
HHHHCCCCCEEEEECCCCEEEEECHHHHHCCCCCEEEECCCEECCCCCCCCHHHHCCCCC
YEVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGFILHAIYKLKNQGEPATFPNIDRML
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
VDPNTNIADLLIEMTQYPHIDGKTHPVISASARDMIDRPEEEAGSVLSTLKSYLALYRDP
ECCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
VVAHNVSASDFCIKDLMHHNNPVSLYIVTQPNDKARLQPLVRVMLNMVVRLLADRMEFER
HHEECCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDDGKGNRFVRTKRNYKHRLLCMIDEFPSLGKLDILQESLAFVAGYGLKFYLICQDINQL
CCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHH
KSRERGYGPDETITSNCHIQNAYPPNRIETAEHLSKLTGQTTIVKEHITTSGKRVSTFLN
HHHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHH
QISKTKQEVSRPLLTIDECQRMPGPKKDANGLITEAGDMVVYVAGFPAIYGKQPLYFKDP
HHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCHHHCCCCCEECCC
VFIARASVESPEQSDILRTRLSEDEEIRL
EEEEECCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SMSKPNKSIGPQVRKKRVEKTNKQIVFLVVLALFVSMQIGTQFVAYKLHYHQSLGFSVA
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEEHHHCCCCEEE
HIYLPWQLLWWNLQYHSIYPDYFNAAFGLVAMSGSLFLMIILFVSKQLTQENVSEYLHGS
EEEECEEEEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ARWANQDDLKDAGLIGNEEGVYVGAVENEKGDIHYLRHNGPEHILTYAPTRSGKGVGLVI
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCEEEECCCCCCCCCEEHH
PTLLSWKQSCVITDLKGELWALTAGWRQKHANNKVIRFEPATLKGSARWNPLDEIRVGTE
HHHHCCCCCEEEEECCCCEEEEECHHHHHCCCCCEEEECCCEECCCCCCCCHHHHCCCCC
YEVGDVQNLATLVVDPDGKGLETHWQKTSQALLVGFILHAIYKLKNQGEPATFPNIDRML
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
VDPNTNIADLLIEMTQYPHIDGKTHPVISASARDMIDRPEEEAGSVLSTLKSYLALYRDP
ECCCCCHHHHHHHHHHCCCCCCCCCCEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
VVAHNVSASDFCIKDLMHHNNPVSLYIVTQPNDKARLQPLVRVMLNMVVRLLADRMEFER
HHEECCCCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDDGKGNRFVRTKRNYKHRLLCMIDEFPSLGKLDILQESLAFVAGYGLKFYLICQDINQL
CCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHH
KSRERGYGPDETITSNCHIQNAYPPNRIETAEHLSKLTGQTTIVKEHITTSGKRVSTFLN
HHHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCEEEHHHHHCCCHHHHHHHH
QISKTKQEVSRPLLTIDECQRMPGPKKDANGLITEAGDMVVYVAGFPAIYGKQPLYFKDP
HHHHHHHHHHCCCEEHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCHHHCCCCCEECCC
VFIARASVESPEQSDILRTRLSEDEEIRL
EEEEECCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1665997; 8021214; 11976307 [H]