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The map label for this gene is traF [H]

Identifier: 148358320

GI number: 148358320

Start: 197566

End: 198111

Strand: Direct

Name: traF [H]

Synonym: LPC_0182

Alternate gene names: 148358320

Gene position: 197566-198111 (Clockwise)

Preceding gene: 148358319

Following gene: 148358321

Centisome position: 5.52

GC content: 40.48

Gene sequence:

>546_bases
ATGAAAAAGTTTATGGCCTGGGTTGCTGTTGTTCTTTTAGGCCTTATCAGCCTATCGTTTCTTTTAATTATTATGGGCTT
TCGAGTCAACATTACGGATTCGATTCCTGTTGGACTTTATCGCATCACCAACTTAAAAAATCTTAAAAATGCTTTTGTGA
TTTTTTGCCCTGATGACAGGCCTGCTTTTAAGCAAGGGTTAGATAGAGGCTATGTTGAGAAAGGCCTTTGTCCTGGTGGT
TATGGGTATTTAATGAAAAAAGTGGTAGCAACCAGCGGTGATAAAGTGTCCGTCATTTCTGAAGGCGTGTTTGTTAATCG
CCAATTTCTTCCCTATTCAAAACCTAAATTAACGGATGGGATGAATTATGCACTGCCACAATGGCGCGCCATCGATTATC
AACTCAAAGAAGATGAAATTCTGACCATGACTAACCAAAGTGAATGGTCATTTGATGCTCGTTATTACGGCCCGATTCGT
TTAGGGCAAATCAAAGGAGTGATCACGCCCGTATGGGTCAAAGCAAAAATGGAGAGAAAGACATGA

Upstream 100 bases:

>100_bases
ATTTCAAAGATCCAGTGTTTATCGCCAGAGCTTCCGTTGAGTCACCTGAGCAATCCGATATCTTACGTACTCGTCTTTCT
GAAGATGAGGAAATCAGGCT

Downstream 100 bases:

>100_bases
ATAAAGATACTGAATTAATGGATGTGATTACAGACAAACTTGAGGATTTGATGGTGCCTGGTTTTGTCGCTGAAATCAGT
CCTTTAGAGGCAGAAATCAT

Product: conjugal transfer peptidase TraF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKKFMAWVAVVLLGLISLSFLLIIMGFRVNITDSIPVGLYRITNLKNLKNAFVIFCPDDRPAFKQGLDRGYVEKGLCPGG
YGYLMKKVVATSGDKVSVISEGVFVNRQFLPYSKPKLTDGMNYALPQWRAIDYQLKEDEILTMTNQSEWSFDARYYGPIR
LGQIKGVITPVWVKAKMERKT

Sequences:

>Translated_181_residues
MKKFMAWVAVVLLGLISLSFLLIIMGFRVNITDSIPVGLYRITNLKNLKNAFVIFCPDDRPAFKQGLDRGYVEKGLCPGG
YGYLMKKVVATSGDKVSVISEGVFVNRQFLPYSKPKLTDGMNYALPQWRAIDYQLKEDEILTMTNQSEWSFDARYYGPIR
LGQIKGVITPVWVKAKMERKT
>Mature_181_residues
MKKFMAWVAVVLLGLISLSFLLIIMGFRVNITDSIPVGLYRITNLKNLKNAFVIFCPDDRPAFKQGLDRGYVEKGLCPGG
YGYLMKKVVATSGDKVSVISEGVFVNRQFLPYSKPKLTDGMNYALPQWRAIDYQLKEDEILTMTNQSEWSFDARYYGPIR
LGQIKGVITPVWVKAKMERKT

Specific function: Required for donor-specific phage sensitivity. May be involved in pilus assembly [H]

COG id: COG4959

COG function: function code OU; Type IV secretory pathway, protease TraF

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR019533
- InterPro:   IPR014139 [H]

Pfam domain/function: PF10502 Peptidase_S26 [H]

EC number: NA

Molecular weight: Translated: 20572; Mature: 20572

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFMAWVAVVLLGLISLSFLLIIMGFRVNITDSIPVGLYRITNLKNLKNAFVIFCPDDR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCCCCCCCEEEEEECCCC
PAFKQGLDRGYVEKGLCPGGYGYLMKKVVATSGDKVSVISEGVFVNRQFLPYSKPKLTDG
HHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCCCCC
MNYALPQWRAIDYQLKEDEILTMTNQSEWSFDARYYGPIRLGQIKGVITPVWVKAKMERK
CCCCCCCCEEECEEECCCEEEEEECCCCCCCCCEEECCEEECCCCCCCCEEEEEEECCCC
T
C
>Mature Secondary Structure
MKKFMAWVAVVLLGLISLSFLLIIMGFRVNITDSIPVGLYRITNLKNLKNAFVIFCPDDR
CHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEEECCCCCCCCEEEEEECCCC
PAFKQGLDRGYVEKGLCPGGYGYLMKKVVATSGDKVSVISEGVFVNRQFLPYSKPKLTDG
HHHHHCCCCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCCCCC
MNYALPQWRAIDYQLKEDEILTMTNQSEWSFDARYYGPIRLGQIKGVITPVWVKAKMERK
CCCCCCCCEEECEEECCCEEEEEECCCCCCCCCEEECCEEECCCCCCCCEEEEEEECCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1400217 [H]