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The map label for this gene is dnaN [H]

Identifier: 148358141

GI number: 148358141

Start: 2027

End: 3130

Strand: Direct

Name: dnaN [H]

Synonym: LPC_0002

Alternate gene names: 148358141

Gene position: 2027-3130 (Clockwise)

Preceding gene: 148358140

Following gene: 148358142

Centisome position: 0.06

GC content: 35.6

Gene sequence:

>1104_bases
TTGTTTGAGCTAACCATTAAAAAAGAAGATCTGCTTACTCCATTGCTTACTGTTTCCGGAGCCGTAGATAAGAAGCAGTC
TTTGGCTATTTTGTCCCATTTTTTATTAAAACTGTCGGATGGACTTTTATATATTACGGCTACAGATTTGGAAATTGAAA
TGTCCGCACAAGTGGCATGTGATTCCAATAATACTAGTGGTTCTATTACAGTACCAGCCAAGAAATTCATTGATATTATC
CGGTCATTGGATGAAAACCCTTCGCCATCTATCGTTTTTGATAAAGCTTTAGTGACGATAAAACAAAACCGAAGTACATT
TAAACTGGCCACTTTGCCTGCTGAGAATTATCCCAGTAGCGAAGATGAATGCAATGATGTCGAGTTGACTATCCCGCGTT
TGGTTTTGTTGCAGTTATTGCAATCTACTCATTTTGCTATGTCTCAGCAGGATGTTCGTATATTTTTGAATGGATTATTG
CTGGAGTTTGACAGTAATATTATTTCAGCGGTTGCTACTGATGGGCACAGAATGGCTATATCGCGATATTCCTGTAATAA
TATTACTCAAACAAAATTACTTATACCCAAAAAAGGGGTGCAAGAATTATTAAGACTATTGAATTGTATTAATGACGAAC
AAGTTTTGTTGGCTGCGGGAAAATCTCATATTAAGTTGATTACTAACCAATTTGTATTTTTGTCTAAATTAATCGAAGCA
CGCTTTCCACCTTATACTAAAGCTATACCAAGAGAACAGGATAAATATATTGTTATTGATTGCGCTACGCTCAAGCGTGC
ATTATCTCGTATTGTCATTTTAGCTCATGAAAAGTCAAAGGCTGTCTTGTTGCATCTACAACCAGGTCAGTTAACTTTAA
TTGCCAATAATAATGAGCAAGAAGAGGCAATTGAAACATTGAGTGCCGAAACACAAGGTGATGAACTCAAAATCGGACTT
AATGCAACCTATCTGCTGGATGTTTTAGCTCACTTTTCTGATGGTCAAATCAGGTTATCAATGTCTAATACTGAAAGCAG
TATTTTAATTGAGTCATTAGTCAACGATAATTATCAATACATTATTATGCCTATGAAAATATGA

Upstream 100 bases:

>100_bases
GCCTGTCGGAAGGTTAAAGAATTAATCCAGGATGATAGCGATTTTGCTGAAGATTATAAAAATTTAATGCGAATATTATC
TTCTTGATACGGAGTCTATC

Downstream 100 bases:

>100_bases
TTTTATCTGAAGTTAGAATTCATAATTTTCGGAATATCGCTTCGACTAGTTTAATCCTAAATCCCAATTTTAATTGTATT
ACAGGACCTAATGGCAGTGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLYITATDLEIEMSAQVACDSNNTSGSITVPAKKFIDII
RSLDENPSPSIVFDKALVTIKQNRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
LEFDSNIISAVATDGHRMAISRYSCNNITQTKLLIPKKGVQELLRLLNCINDEQVLLAAGKSHIKLITNQFVFLSKLIEA
RFPPYTKAIPREQDKYIVIDCATLKRALSRIVILAHEKSKAVLLHLQPGQLTLIANNNEQEEAIETLSAETQGDELKIGL
NATYLLDVLAHFSDGQIRLSMSNTESSILIESLVNDNYQYIIMPMKI

Sequences:

>Translated_367_residues
MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLYITATDLEIEMSAQVACDSNNTSGSITVPAKKFIDII
RSLDENPSPSIVFDKALVTIKQNRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
LEFDSNIISAVATDGHRMAISRYSCNNITQTKLLIPKKGVQELLRLLNCINDEQVLLAAGKSHIKLITNQFVFLSKLIEA
RFPPYTKAIPREQDKYIVIDCATLKRALSRIVILAHEKSKAVLLHLQPGQLTLIANNNEQEEAIETLSAETQGDELKIGL
NATYLLDVLAHFSDGQIRLSMSNTESSILIESLVNDNYQYIIMPMKI
>Mature_367_residues
MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLYITATDLEIEMSAQVACDSNNTSGSITVPAKKFIDII
RSLDENPSPSIVFDKALVTIKQNRSTFKLATLPAENYPSSEDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLL
LEFDSNIISAVATDGHRMAISRYSCNNITQTKLLIPKKGVQELLRLLNCINDEQVLLAAGKSHIKLITNQFVFLSKLIEA
RFPPYTKAIPREQDKYIVIDCATLKRALSRIVILAHEKSKAVLLHLQPGQLTLIANNNEQEEAIETLSAETQGDELKIGL
NATYLLDVLAHFSDGQIRLSMSNTESSILIESLVNDNYQYIIMPMKI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=37.874659400545, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40898; Mature: 40898

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLYITATDLEIEMSAQVAC
CEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECEEEEEEEEEEE
DSNNTSGSITVPAKKFIDIIRSLDENPSPSIVFDKALVTIKQNRSTFKLATLPAENYPSS
CCCCCCCEEEECHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCC
EDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLLLEFDSNIISAVATDGHRMAI
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCHHHHHHCCCCCEEEE
SRYSCNNITQTKLLIPKKGVQELLRLLNCINDEQVLLAAGKSHIKLITNQFVFLSKLIEA
EEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHH
RFPPYTKAIPREQDKYIVIDCATLKRALSRIVILAHEKSKAVLLHLQPGQLTLIANNNEQ
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHEEEEEEECCCCEEEEEECCCEEEEEECCCCH
EEAIETLSAETQGDELKIGLNATYLLDVLAHFSDGQIRLSMSNTESSILIESLVNDNYQY
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEE
IIMPMKI
EEEEECC
>Mature Secondary Structure
MFELTIKKEDLLTPLLTVSGAVDKKQSLAILSHFLLKLSDGLLYITATDLEIEMSAQVAC
CEEEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECEEEEEEEEEEE
DSNNTSGSITVPAKKFIDIIRSLDENPSPSIVFDKALVTIKQNRSTFKLATLPAENYPSS
CCCCCCCEEEECHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCC
EDECNDVELTIPRLVLLQLLQSTHFAMSQQDVRIFLNGLLLEFDSNIISAVATDGHRMAI
CCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCHHHHHHCCCCCEEEE
SRYSCNNITQTKLLIPKKGVQELLRLLNCINDEQVLLAAGKSHIKLITNQFVFLSKLIEA
EEECCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHHHHHHHHHHHHH
RFPPYTKAIPREQDKYIVIDCATLKRALSRIVILAHEKSKAVLLHLQPGQLTLIANNNEQ
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHEEEEEEECCCCEEEEEECCCEEEEEECCCCH
EEAIETLSAETQGDELKIGLNATYLLDVLAHFSDGQIRLSMSNTESSILIESLVNDNYQY
HHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEE
IIMPMKI
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]