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The map label for this gene is recF [H]

Identifier: 148358142

GI number: 148358142

Start: 3127

End: 4188

Strand: Direct

Name: recF [H]

Synonym: LPC_0003

Alternate gene names: 148358142

Gene position: 3127-4188 (Clockwise)

Preceding gene: 148358141

Following gene: 148358143

Centisome position: 0.09

GC content: 35.31

Gene sequence:

>1062_bases
ATGATTTTATCTGAAGTTAGAATTCATAATTTTCGGAATATCGCTTCGACTAGTTTAATCCTAAATCCCAATTTTAATTG
TATTACAGGACCTAATGGCAGTGGTAAAACATCGTTATTAGAGGCTCTTTACATGTTGAGCTGCGCCCATTCTTTTCGGT
CTCGTGAAGTAGCTCCAATTATTTCTTATGGTCAAAACCAACTTAATGTTTTTGCCCATACTTACGACGAAAGCACTATT
AGTGTTCAGAAATCTATAACAGATGGCACACAAATTAAATTGAATAACCAGTTTTGTTGTACTACAAGTCAACTAGCCTA
TGCGCTTCCTTGCCAGGTTATTTATTCTGATATTTTTCAAATTATTGATGCCGGCCCCTCGGTAAGGAGAAGCTTACTTG
ATTGGGGATTGTTTCACGTGAAACATGATTATTTGAAAATTTGGAAGGATTACAAGCGCATACTTAGCCAACGAAATGCC
TTATTAAAATCCAGAGCTACTTATGAACATTTTATTCCTTGGGACCAACAGCTAAGTCAATTGGCAAATCAACTGGATAA
AGCTAGAAATGACTATTTTTTGCAATGGCAACCTAAATTCTACCAAGTCTTAAGTGATTTAACCAATATATCCTGCACAA
TTGAGTATTACAAAGGGTGGGATAGAAAAAATGCAGGGCAGAACATGGAGGAACTACTTCAGAAATCCTTTGATAGTGAT
AAAAATAAGCTATATACGCAATATGGACCCCACCAAGCTGATTTGATAATTAACATTGAGCAATACAGAGTAAAGCATAC
ACTCTCTCGTGGACAACAGAAAATCATTCTAATCGCACTTAAATTGGCTCAAGGTCAGCTATTAGATAAGGATTGCTTAT
ACCTTATTGATGATTTGGCTGCTGAGTTAGATGACTATCACCAGCGAAATTTAATTAAGTATCTGACACAGCAGAAGGGT
CAGTTTGTTATAACAAATTTAATTAACAACAATAATTACGATATCTTGCCAATTGACTCTGGTTTGTTTGAAGTAAACTG
TGGCGCAATTAAAGTTTTATAA

Upstream 100 bases:

>100_bases
ATGGTCAAATCAGGTTATCAATGTCTAATACTGAAAGCAGTATTTTAATTGAGTCATTAGTCAACGATAATTATCAATAC
ATTATTATGCCTATGAAAAT

Downstream 100 bases:

>100_bases
TATAGAATGTTTCACGTGGAACACAAATTAATGGAATGAAAAGTTCTCGTTATTAAACAATAACACCTTAATGCAAATGC
AATATAAAATTCTAAAGGTA

Product: DNA recombination/repair protein ATPase RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVAPIISYGQNQLNVFAHTYDESTI
SVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTNISCTIEYYKGWDRKNAGQNMEELLQKSFDSD
KNKLYTQYGPHQADLIINIEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLTQQKG
QFVITNLINNNNYDILPIDSGLFEVNCGAIKVL

Sequences:

>Translated_353_residues
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVAPIISYGQNQLNVFAHTYDESTI
SVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTNISCTIEYYKGWDRKNAGQNMEELLQKSFDSD
KNKLYTQYGPHQADLIINIEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLTQQKG
QFVITNLINNNNYDILPIDSGLFEVNCGAIKVL
>Mature_353_residues
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVAPIISYGQNQLNVFAHTYDESTI
SVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQIIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNA
LLKSRATYEHFIPWDQQLSQLANQLDKARNDYFLQWQPKFYQVLSDLTNISCTIEYYKGWDRKNAGQNMEELLQKSFDSD
KNKLYTQYGPHQADLIINIEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLAAELDDYHQRNLIKYLTQQKG
QFVITNLINNNNYDILPIDSGLFEVNCGAIKVL

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP [H]

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family [H]

Homologues:

Organism=Escherichia coli, GI1790135, Length=358, Percent_Identity=34.3575418994413, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 40723; Mature: 40723

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVAPI
CCCCHHHHHHHHHHHCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
ISYGQNQLNVFAHTYDESTISVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQ
HHCCCCEEEEEEEECCCCCEEEEHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHH
IIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNALLKSRATYEHFIPWDQQLSQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHH
LANQLDKARNDYFLQWQPKFYQVLSDLTNISCTIEYYKGWDRKNAGQNMEELLQKSFDSD
HHHHHHHCCCCCEEEECHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCC
KNKLYTQYGPHQADLIINIEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLA
CCEEEECCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHH
AELDDYHQRNLIKYLTQQKGQFVITNLINNNNYDILPIDSGLFEVNCGAIKVL
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEECCCEEEEC
>Mature Secondary Structure
MILSEVRIHNFRNIASTSLILNPNFNCITGPNGSGKTSLLEALYMLSCAHSFRSREVAPI
CCCCHHHHHHHHHHHCEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHH
ISYGQNQLNVFAHTYDESTISVQKSITDGTQIKLNNQFCCTTSQLAYALPCQVIYSDIFQ
HHCCCCEEEEEEEECCCCCEEEEHHCCCCCEEEECCEEEEEHHHHHHHHHHHHHHHHHHH
IIDAGPSVRRSLLDWGLFHVKHDYLKIWKDYKRILSQRNALLKSRATYEHFIPWDQQLSQ
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHH
LANQLDKARNDYFLQWQPKFYQVLSDLTNISCTIEYYKGWDRKNAGQNMEELLQKSFDSD
HHHHHHHCCCCCEEEECHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCC
KNKLYTQYGPHQADLIINIEQYRVKHTLSRGQQKIILIALKLAQGQLLDKDCLYLIDDLA
CCEEEECCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHH
AELDDYHQRNLIKYLTQQKGQFVITNLINNNNYDILPIDSGLFEVNCGAIKVL
HHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA