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The map label for this gene is proP [H]

Identifier: 148285055

GI number: 148285055

Start: 1891730

End: 1892983

Strand: Direct

Name: proP [H]

Synonym: OTBS_1955

Alternate gene names: 148285055

Gene position: 1891730-1892983 (Clockwise)

Preceding gene: 148285054

Following gene: 148285056

Centisome position: 88.94

GC content: 30.22

Gene sequence:

>1254_bases
ATGAACAAGAAGGTTCTAATTTTATCTATGCTTGTAACTTTAGCTCATTATTATGATTATCATTTATTTGGCTTATTAGC
TTCTCAAATTTCTGAGTGTTTTATTACTAAAAGTGATAGTATTACACAATTAAGGAATACTTATCTTATTATGGGAATAG
CTATTTTTGGTAAAGCAATTGGAGCTGTTATTTTGGGGAGAATAGGTGATCTTTATGGTCGAGATGCTACTTTTAGTATA
AGCTTAATAGGAACAGCTAGTGGGTCGTTATTAATTGCTATTTTACCATGTTATAATATCATTGGAGTGTTATCAGTTTT
CGGCTTATTATTGGCTAGAATTTCTATATGTATGTTTACTTCGCCAGGAACTGATGGAGTAAGATTATATGTATATGAAA
CTATAGGTAATAAATATAAATGCTTAGGTGGAGGTATAGTTAATGCTTCAACAAACTGTGGGTCATTTATTGCTTCTATA
TCAGCACTGTTTTTTACTTTAGATATCATGCCTAAATATAGCTGGCGCTTTGCTTTTATACTTGGAGGACTATTTGGAGT
ATGTGTGGTAATTATACGCAGGATGTTTATTCAATCTATTCAAGATGATCAAGTAAAAAATTCTCAGTATCATGAATATA
AAGATTGTTCAATTTTGCAGATTGTGCGTAAGAATTTAAATGTATTTATTTTAGCTGTATTAATAGCTGGTACTATTGGT
AGCACTAATCAATTTTGCACGATATTTTTTGGTACCTATATTTTTAAAATATTGCAATATTTAGATGAAGCTACAGCGAA
ATATTATGTTACGCTTGGAATCGCAATACAGATGTTATTTGCAATTATAGGAGGATATACTGCTGATATTATAGGTAAAA
AATTAGTTTCTTCTATTGCTTTTTGTTGCTTAGGAATAGCTGTCATTATTATGGCTTATATGATAAATTTAGGCAACTTT
ATTCCAGTATTATACCTGACTATATGTATGCTTGTACCGTTTTTAACTATGCCAGCTTTAGTTTTTCTTAAGGAAAGTAT
TCCTAAGACTATAAGATATAGATTATTTAGTGCTGCGCATGCTTGTGGATCAAGCTTAATTTCTGGTCCAACAACGTTTG
TTGCGACTAGCTTATATCAGATAGGTAGTGCATGGTGGCCAATGATGTATTTTTTAGTAATAATAGTTATTATGATTACA
GTAATTTATTTGCTTGATTATTGTAGCAAAAATCAGCAAAATCTAAGTTTCTAA

Upstream 100 bases:

>100_bases
TAAGGGAATAAATAGAAAGGGAATGAGCTATGTAAAAAGTATCATGATGCAACTTTTAAGTTAGTGTCATGATATTCAGT
ATTTTAGGAGAATAAGATTT

Downstream 100 bases:

>100_bases
TTTAAATGTATGGTAGTGGTAAAGTTGTATGGGTGTAATAAAATATTTGTCTGATACTACTATAAACCGTATAGCTGCTG
GAGAGGTAGTAGAGCGTCCT

Product: proline/betaine transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNKKVLILSMLVTLAHYYDYHLFGLLASQISECFITKSDSITQLRNTYLIMGIAIFGKAIGAVILGRIGDLYGRDATFSI
SLIGTASGSLLIAILPCYNIIGVLSVFGLLLARISICMFTSPGTDGVRLYVYETIGNKYKCLGGGIVNASTNCGSFIASI
SALFFTLDIMPKYSWRFAFILGGLFGVCVVIIRRMFIQSIQDDQVKNSQYHEYKDCSILQIVRKNLNVFILAVLIAGTIG
STNQFCTIFFGTYIFKILQYLDEATAKYYVTLGIAIQMLFAIIGGYTADIIGKKLVSSIAFCCLGIAVIIMAYMINLGNF
IPVLYLTICMLVPFLTMPALVFLKESIPKTIRYRLFSAAHACGSSLISGPTTFVATSLYQIGSAWWPMMYFLVIIVIMIT
VIYLLDYCSKNQQNLSF

Sequences:

>Translated_417_residues
MNKKVLILSMLVTLAHYYDYHLFGLLASQISECFITKSDSITQLRNTYLIMGIAIFGKAIGAVILGRIGDLYGRDATFSI
SLIGTASGSLLIAILPCYNIIGVLSVFGLLLARISICMFTSPGTDGVRLYVYETIGNKYKCLGGGIVNASTNCGSFIASI
SALFFTLDIMPKYSWRFAFILGGLFGVCVVIIRRMFIQSIQDDQVKNSQYHEYKDCSILQIVRKNLNVFILAVLIAGTIG
STNQFCTIFFGTYIFKILQYLDEATAKYYVTLGIAIQMLFAIIGGYTADIIGKKLVSSIAFCCLGIAVIIMAYMINLGNF
IPVLYLTICMLVPFLTMPALVFLKESIPKTIRYRLFSAAHACGSSLISGPTTFVATSLYQIGSAWWPMMYFLVIIVIMIT
VIYLLDYCSKNQQNLSF
>Mature_417_residues
MNKKVLILSMLVTLAHYYDYHLFGLLASQISECFITKSDSITQLRNTYLIMGIAIFGKAIGAVILGRIGDLYGRDATFSI
SLIGTASGSLLIAILPCYNIIGVLSVFGLLLARISICMFTSPGTDGVRLYVYETIGNKYKCLGGGIVNASTNCGSFIASI
SALFFTLDIMPKYSWRFAFILGGLFGVCVVIIRRMFIQSIQDDQVKNSQYHEYKDCSILQIVRKNLNVFILAVLIAGTIG
STNQFCTIFFGTYIFKILQYLDEATAKYYVTLGIAIQMLFAIIGGYTADIIGKKLVSSIAFCCLGIAVIIMAYMINLGNF
IPVLYLTICMLVPFLTMPALVFLKESIPKTIRYRLFSAAHACGSSLISGPTTFVATSLYQIGSAWWPMMYFLVIIVIMIT
VIYLLDYCSKNQQNLSF

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=390, Percent_Identity=24.8717948717949, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=19.7149643705463, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46099; Mature: 46099

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKVLILSMLVTLAHYYDYHLFGLLASQISECFITKSDSITQLRNTYLIMGIAIFGKAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GAVILGRIGDLYGRDATFSISLIGTASGSLLIAILPCYNIIGVLSVFGLLLARISICMFT
HHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPGTDGVRLYVYETIGNKYKCLGGGIVNASTNCGSFIASISALFFTLDIMPKYSWRFAFI
CCCCCCEEEEEEECCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LGGLFGVCVVIIRRMFIQSIQDDQVKNSQYHEYKDCSILQIVRKNLNVFILAVLIAGTIG
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC
STNQFCTIFFGTYIFKILQYLDEATAKYYVTLGIAIQMLFAIIGGYTADIIGKKLVSSIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FCCLGIAVIIMAYMINLGNFIPVLYLTICMLVPFLTMPALVFLKESIPKTIRYRLFSAAH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACGSSLISGPTTFVATSLYQIGSAWWPMMYFLVIIVIMITVIYLLDYCSKNQQNLSF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNKKVLILSMLVTLAHYYDYHLFGLLASQISECFITKSDSITQLRNTYLIMGIAIFGKAI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GAVILGRIGDLYGRDATFSISLIGTASGSLLIAILPCYNIIGVLSVFGLLLARISICMFT
HHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SPGTDGVRLYVYETIGNKYKCLGGGIVNASTNCGSFIASISALFFTLDIMPKYSWRFAFI
CCCCCCEEEEEEECCCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LGGLFGVCVVIIRRMFIQSIQDDQVKNSQYHEYKDCSILQIVRKNLNVFILAVLIAGTIG
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC
STNQFCTIFFGTYIFKILQYLDEATAKYYVTLGIAIQMLFAIIGGYTADIIGKKLVSSIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FCCLGIAVIIMAYMINLGNFIPVLYLTICMLVPFLTMPALVFLKESIPKTIRYRLFSAAH
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACGSSLISGPTTFVATSLYQIGSAWWPMMYFLVIIVIMITVIYLLDYCSKNQQNLSF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]