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The map label for this gene is nuoL2 [H]

Identifier: 148284773

GI number: 148284773

Start: 1386663

End: 1388135

Strand: Direct

Name: nuoL2 [H]

Synonym: OTBS_1426

Alternate gene names: 148284773

Gene position: 1386663-1388135 (Clockwise)

Preceding gene: 148284772

Following gene: 148284774

Centisome position: 65.19

GC content: 28.58

Gene sequence:

>1473_bases
ATGCTTAAAAACATTATTTTATTATACAACGACATGATATACTATATTCCATTTACTATAATCGGTATAGTAGGTATATG
GAATTTAATTATTCCATTTCTGATTCGCAAAAATTTTATTAAAGAAATATTAATGCTTGCAATATCCATATTGCATGCTA
TTAATGCAATACTATTGTATTTTAGTTATAAAGCCGGGTATGAGCACTATACTACTATTTTCAGTATCGGAGCATTTGAT
TTTAGCCTAAGATTAAGTTTATTAGGTAGTAACATACTTTTACTAATAGCAATATTGTGGCCAATATCAATTATATATAC
TATTGGTTTTTTGAAAGCTCATAGTGAGCCAAATCAGGCTAAATTTTTACTTTGCACTAATCTATGTATATTAATTAGTA
ACTATATTGTATGTTCTGCTAATTTTTTTACTATGTTGATATGTTACGAACTTTTAACCTTTGCCACAATTCCATTAATA
AGGCATGTAACTTCTCATAGAACTAGCAAAGTTTTGAGTAAATACACTCAAGGATTATATTTACCATCTTTACTGTTTTT
AATGCCAACCTTAGTAGCTTTGCAATATCAATATAGTACAACAGATTTTGTAGAAATTGCTAACATATTACAATCACGTA
CTCTATTACAAGTAAGTCCTGTTATTTTTGCATTATGTATACTGTGTGGTACTGCTAAAGCAGCTATTGTCCCTTTTCAT
AGCTGGCTTCCACCTGCTATGATAGCTCCATATCCAGTAAGCGCAATTTTACATGCTGTTGCAGTTGTTAATGTTGGTAT
TTTTTGCTTGATAACTGGAATCCTCTCAGTATTAGGAAAGGAAACATGGTTATTAAATTATATACAGTATTTTATAGGAT
ATGGAATTGTATATAGCAGCATAAAGGCATTATTTCAAACTAATATAAAAAGAATTTTGGCTTACTCAACTATATCTAAC
TTAGGTATAATAGCTTTAACAGCATTTATGAATAGCCATGAATCTTTACTTGCTACTATTACTCACATAATTGCTCATTC
AATAGGTAAGATTACAGTATTCTATTCTGCAGGTTGTTTATATACAATTAGTAAATGTACTAATGTTGAAGATTTAACAT
CAATGTCTTATAAAGCTCCTATAATAACATCATGCCTAACAATAGGAAGTTTATCATTAATAGGCATACCACCATTAGCA
GGCTTTTTCAGTAAACATCATATAATCCTAGAAGCAGCAAATGAACATAATTATTTAGTAATCGCTATTACAGTTTTTTC
AGGAATAACTTCATGCTTGTATTTATCAAAAATAATAAAAACTGCCTATAGCAAAATAGATAATGAATGTAATATTAACT
CTGCAAATAACATTCATTCAGAACATATGATACCTAAACATATGCTTTATAGCACGGTATCATGTGCTAGTTTAGTTGTA
TTAATTCCTATTATTAATGCTTTAATAATGTAA

Upstream 100 bases:

>100_bases
AATTTTAACTATTATCAGCTATTTGATGCTAATATTTTACAATCACTTATTAGTATACTTAAAGCCGTTTTTTCTACCAA
AAATCATGTGAGTTTTATAA

Downstream 100 bases:

>100_bases
CATGATTGACAGAACTGTCAACTATCAGTATATTGCTATAATATTAGATTGATGGACGAAGATTTAAAATGCAACAATGT
TTTCTTACTTTAACTGTATT

Product: NADH dehydrogenase I chain L

Products: NAD+; ubiquinol

Alternate protein names: Mrp complex subunit A2; Putative NADH-ubiquinone oxidoreductase subunit mnhA2 [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MLKNIILLYNDMIYYIPFTIIGIVGIWNLIIPFLIRKNFIKEILMLAISILHAINAILLYFSYKAGYEHYTTIFSIGAFD
FSLRLSLLGSNILLLIAILWPISIIYTIGFLKAHSEPNQAKFLLCTNLCILISNYIVCSANFFTMLICYELLTFATIPLI
RHVTSHRTSKVLSKYTQGLYLPSLLFLMPTLVALQYQYSTTDFVEIANILQSRTLLQVSPVIFALCILCGTAKAAIVPFH
SWLPPAMIAPYPVSAILHAVAVVNVGIFCLITGILSVLGKETWLLNYIQYFIGYGIVYSSIKALFQTNIKRILAYSTISN
LGIIALTAFMNSHESLLATITHIIAHSIGKITVFYSAGCLYTISKCTNVEDLTSMSYKAPIITSCLTIGSLSLIGIPPLA
GFFSKHHIILEAANEHNYLVIAITVFSGITSCLYLSKIIKTAYSKIDNECNINSANNIHSEHMIPKHMLYSTVSCASLVV
LIPIINALIM

Sequences:

>Translated_490_residues
MLKNIILLYNDMIYYIPFTIIGIVGIWNLIIPFLIRKNFIKEILMLAISILHAINAILLYFSYKAGYEHYTTIFSIGAFD
FSLRLSLLGSNILLLIAILWPISIIYTIGFLKAHSEPNQAKFLLCTNLCILISNYIVCSANFFTMLICYELLTFATIPLI
RHVTSHRTSKVLSKYTQGLYLPSLLFLMPTLVALQYQYSTTDFVEIANILQSRTLLQVSPVIFALCILCGTAKAAIVPFH
SWLPPAMIAPYPVSAILHAVAVVNVGIFCLITGILSVLGKETWLLNYIQYFIGYGIVYSSIKALFQTNIKRILAYSTISN
LGIIALTAFMNSHESLLATITHIIAHSIGKITVFYSAGCLYTISKCTNVEDLTSMSYKAPIITSCLTIGSLSLIGIPPLA
GFFSKHHIILEAANEHNYLVIAITVFSGITSCLYLSKIIKTAYSKIDNECNINSANNIHSEHMIPKHMLYSTVSCASLVV
LIPIINALIM
>Mature_490_residues
MLKNIILLYNDMIYYIPFTIIGIVGIWNLIIPFLIRKNFIKEILMLAISILHAINAILLYFSYKAGYEHYTTIFSIGAFD
FSLRLSLLGSNILLLIAILWPISIIYTIGFLKAHSEPNQAKFLLCTNLCILISNYIVCSANFFTMLICYELLTFATIPLI
RHVTSHRTSKVLSKYTQGLYLPSLLFLMPTLVALQYQYSTTDFVEIANILQSRTLLQVSPVIFALCILCGTAKAAIVPFH
SWLPPAMIAPYPVSAILHAVAVVNVGIFCLITGILSVLGKETWLLNYIQYFIGYGIVYSSIKALFQTNIKRILAYSTISN
LGIIALTAFMNSHESLLATITHIIAHSIGKITVFYSAGCLYTISKCTNVEDLTSMSYKAPIITSCLTIGSLSLIGIPPLA
GFFSKHHIILEAANEHNYLVIAITVFSGITSCLYLSKIIKTAYSKIDNECNINSANNIHSEHMIPKHMLYSTVSCASLVV
LIPIINALIM

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=234, Percent_Identity=34.6153846153846, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1788614, Length=383, Percent_Identity=27.154046997389, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1788829, Length=347, Percent_Identity=26.2247838616715, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788827, Length=435, Percent_Identity=22.2988505747126, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI145693160, Length=465, Percent_Identity=22.7956989247312, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788831, Length=355, Percent_Identity=25.9154929577465, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI2367154, Length=229, Percent_Identity=26.2008733624454, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54445; Mature: 54445

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNIILLYNDMIYYIPFTIIGIVGIWNLIIPFLIRKNFIKEILMLAISILHAINAILLY
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSYKAGYEHYTTIFSIGAFDFSLRLSLLGSNILLLIAILWPISIIYTIGFLKAHSEPNQA
HHHHCCCHHHHHHHEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KFLLCTNLCILISNYIVCSANFFTMLICYELLTFATIPLIRHVTSHRTSKVLSKYTQGLY
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LPSLLFLMPTLVALQYQYSTTDFVEIANILQSRTLLQVSPVIFALCILCGTAKAAIVPFH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECH
SWLPPAMIAPYPVSAILHAVAVVNVGIFCLITGILSVLGKETWLLNYIQYFIGYGIVYSS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IKALFQTNIKRILAYSTISNLGIIALTAFMNSHESLLATITHIIAHSIGKITVFYSAGCL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCHH
YTISKCTNVEDLTSMSYKAPIITSCLTIGSLSLIGIPPLAGFFSKHHIILEAANEHNYLV
HHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCEECCCCHHHHHCCCEEEEEECCCCCEEE
IAITVFSGITSCLYLSKIIKTAYSKIDNECNINSANNIHSEHMIPKHMLYSTVSCASLVV
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
LIPIINALIM
HHHHHHHHCC
>Mature Secondary Structure
MLKNIILLYNDMIYYIPFTIIGIVGIWNLIIPFLIRKNFIKEILMLAISILHAINAILLY
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSYKAGYEHYTTIFSIGAFDFSLRLSLLGSNILLLIAILWPISIIYTIGFLKAHSEPNQA
HHHHCCCHHHHHHHEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KFLLCTNLCILISNYIVCSANFFTMLICYELLTFATIPLIRHVTSHRTSKVLSKYTQGLY
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
LPSLLFLMPTLVALQYQYSTTDFVEIANILQSRTLLQVSPVIFALCILCGTAKAAIVPFH
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEECH
SWLPPAMIAPYPVSAILHAVAVVNVGIFCLITGILSVLGKETWLLNYIQYFIGYGIVYSS
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
IKALFQTNIKRILAYSTISNLGIIALTAFMNSHESLLATITHIIAHSIGKITVFYSAGCL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCHH
YTISKCTNVEDLTSMSYKAPIITSCLTIGSLSLIGIPPLAGFFSKHHIILEAANEHNYLV
HHHHHCCCHHHHHHCCCCCHHHHHHHHHCCCCEECCCCHHHHHCCCEEEEEECCCCCEEE
IAITVFSGITSCLYLSKIIKTAYSKIDNECNINSANNIHSEHMIPKHMLYSTVSCASLVV
EEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHH
LIPIINALIM
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA