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The map label for this gene is dam21 [H]

Identifier: 148284774

GI number: 148284774

Start: 1391245

End: 1391685

Strand: Direct

Name: dam21 [H]

Synonym: OTBS_1432

Alternate gene names: 148284774

Gene position: 1391245-1391685 (Clockwise)

Preceding gene: 148284773

Following gene: 148284776

Centisome position: 65.41

GC content: 32.2

Gene sequence:

>441_bases
TTGGCGTTTAGAGGAATTTATAGGCTAAACAGAGACGGCACATCTGCTCAAACATTTTCTGATAAGCAATATCTTAAGCT
TCATATTTGCTCTAGAATAAATAAATGTAGCAAACTTTTAACCGATGTATCAATTTGTGCTATGGATTTTTCATTTATAG
AGCCTAAAAAAGGTGACTTCGTTTATCTTGATCCACCTTATCACCAATCAGGAGAACGGTTCTACACTAGAGTCCCATTT
GATGACAAAGAGCAAATCAGACTACGAGATTTTGTTTATGAACTAAACAATAAAGGTGTTAAGATTATGATCTCAAATAA
TAATACTGCCTTTATTAGAGACTTATACAAAGAGTTATTCATCACTCATATTGGAGTTACATACTCAATCAATGAACAGC
GAAATCTCGTTAATGAGCTAATCATTACTAACTATAATTAA

Upstream 100 bases:

>100_bases
TTCTTTGTTCATATAATTGCTTCTCTTGCTTCTTATTCTATCTCCAAACTTAATCCCTATCTTATCTCCTCTTCTTTTTC
TTCTCTCAACTTATCCTAAA

Downstream 100 bases:

>100_bases
CCAGAGCTCGATATAAAAAATAGAAAAAAAAGAATAAAGAAGGCGTATTAAAAACTACTAGATTCATAAAAAGAAACAAG
TAGAACTGTTATGATGCATT

Product: site-specific DNA adenine methylase

Products: NA

Alternate protein names: M.LlaDCHI A; M.LlaDCHIA; Adenine-specific methyltransferase LlaDCHIA; M.LlaII A [H]

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MAFRGIYRLNRDGTSAQTFSDKQYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPF
DDKEQIRLRDFVYELNNKGVKIMISNNNTAFIRDLYKELFITHIGVTYSINEQRNLVNELIITNYN

Sequences:

>Translated_146_residues
MAFRGIYRLNRDGTSAQTFSDKQYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPF
DDKEQIRLRDFVYELNNKGVKIMISNNNTAFIRDLYKELFITHIGVTYSINEQRNLVNELIITNYN
>Mature_145_residues
AFRGIYRLNRDGTSAQTFSDKQYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKGDFVYLDPPYHQSGERFYTRVPFD
DKEQIRLRDFVYELNNKGVKIMISNNNTAFIRDLYKELFITHIGVTYSINEQRNLVNELIITNYN

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the LlaDCHI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 17225; Mature: 17094

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFRGIYRLNRDGTSAQTFSDKQYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKGDF
CCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCE
VYLDPPYHQSGERFYTRVPFDDKEQIRLRDFVYELNNKGVKIMISNNNTAFIRDLYKELF
EEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
ITHIGVTYSINEQRNLVNELIITNYN
HHHCCEEEEECHHHHHHHHHEEECCC
>Mature Secondary Structure 
AFRGIYRLNRDGTSAQTFSDKQYLKLHICSRINKCSKLLTDVSICAMDFSFIEPKKGDF
CCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCE
VYLDPPYHQSGERFYTRVPFDDKEQIRLRDFVYELNNKGVKIMISNNNTAFIRDLYKELF
EEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
ITHIGVTYSINEQRNLVNELIITNYN
HHHCCEEEEECHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7793939; 11467810 [H]