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The map label for this gene is nuoN2 [H]

Identifier: 148284772

GI number: 148284772

Start: 1385163

End: 1386653

Strand: Direct

Name: nuoN2 [H]

Synonym: OTBS_1425

Alternate gene names: 148284772

Gene position: 1385163-1386653 (Clockwise)

Preceding gene: 148284771

Following gene: 148284773

Centisome position: 65.12

GC content: 28.03

Gene sequence:

>1491_bases
ATGAGTAATATTTTTCAGGCTGTTAAAGACCATTTTGTGATATTAGAAGTTTTAATACCATTTTTTGGTAGTTTAATAGT
AGTATTATATAAAAAAGTTGAGTACGCACTAATAGTATCACGTTTATGTGCAATATTAGGAATAATATTAGGAATATATG
GGGTATTATCAATGATAAATTCACCTCCGTATTTTTATGCAATTGGTAATTGGCAGGCACCGATAGGTATAGAATATCGA
GTTGATCATGTTAATCAAATAATTATCGTTTTTAGTTATTTTATTCTATTTTTATTACTAACATTTAATTCTCAGCTCAT
AAAAACAAACTTATTATCATCTCTTAATCCATCTCGATCTCATTTACTATATTCCATCTTACTATTATTGCACACAGGAT
TTTGTGGTATTATTCTTAGTAATGATTTATTTAATATTTACGTTTTTATAGAAATTGCTTCATTAGCAAGTTACACTTTG
GTTTCTCAGGGAGTTGATAAACGAGCTTTAATTGGTAGTATTAATTATTTAATACTTGGTACTATTGGTGCTACTTTTAT
ACTTATTGCTATAGGATTCATATTGAGTAATACTGGCAGCTTGAATATTACTGATATAATTTCCAGGTTACCACAACAAC
ATACAAAAACCTTAATTGCAGCAATAATGTTTTATATTACTGGATGCTTATTAAAGGTAGCTATGTTTCCTATGAGTTCA
TGGCTTATTAGTACTTATAAATATACTTCTTCAGTAATTTTATCATATTTTGCTCCAATTTCATGTATGGTTGGATTTTA
TATTCTACTATATTTTGTCTATCAAATTATTGGAATTACTAATATTAATACCTTGCATTCATATTATATAATCAATGCCC
TAGGGTTAGCAGGAGCAATTTTTTGTTCTTGTTATGCTGTGCAACAAAGCAACCTCAAATCAATTATGGCATATTCTGTA
TTAGCAGAAACAAGTTACATATCTCTAATGGCATTTAACATACAAAGCTCCACTATTGGTGCGCAAATTATTGTTATACT
GTTAACTAGTTGTATACTTAAATCCATAATATATTCTTTAATTAGCAGAATAGAAGTACATTATCATGATAGCGAGCTTA
AACATATTGCAGGTATTGGATCGACTTATCCAGTGTTTGGTATATTATTTACATTAGCTTTAATAAGTAACTTGGGATTA
CCGCTGACTATTGGCTTTGTTAATAAATTAAACATTTTTTTAACGGTTTTACAGTCACTTGGCTATTTTGGTATAATAAT
CTTGGCTATTAGTAGCTGCATGAGTTTTAATTATCATTATAAAATTTTTCATTATCTATATTCTACTCAGCAAACTTCTC
ATAGCAGCTTTACACTTAATGGTAACAACAGTGTAGCAATAATTTTAACTATTATCAGCTATTTGATGCTAATATTTTAC
AATCACTTATTAGTATACTTAAAGCCGTTTTTTCTACCAAAAATCATGTGA

Upstream 100 bases:

>100_bases
TCTGTAAGTCTTAGTATCATTCGTCAAATTTACCAAAGTTTTAATAGCTGTAATGAAAATGATATTCTATTAGAGCTACA
AAAAATGGAGTTAAAGCCAG

Downstream 100 bases:

>100_bases
GTTTTATAAATGCTTAAAAACATTATTTTATTATACAACGACATGATATACTATATTCCATTTACTATAATCGGTATAGT
AGGTATATGGAATTTAATTA

Product: putative monovalent cation/H+ antiporter subunit D

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MSNIFQAVKDHFVILEVLIPFFGSLIVVLYKKVEYALIVSRLCAILGIILGIYGVLSMINSPPYFYAIGNWQAPIGIEYR
VDHVNQIIIVFSYFILFLLLTFNSQLIKTNLLSSLNPSRSHLLYSILLLLHTGFCGIILSNDLFNIYVFIEIASLASYTL
VSQGVDKRALIGSINYLILGTIGATFILIAIGFILSNTGSLNITDIISRLPQQHTKTLIAAIMFYITGCLLKVAMFPMSS
WLISTYKYTSSVILSYFAPISCMVGFYILLYFVYQIIGITNINTLHSYYIINALGLAGAIFCSCYAVQQSNLKSIMAYSV
LAETSYISLMAFNIQSSTIGAQIIVILLTSCILKSIIYSLISRIEVHYHDSELKHIAGIGSTYPVFGILFTLALISNLGL
PLTIGFVNKLNIFLTVLQSLGYFGIIILAISSCMSFNYHYKIFHYLYSTQQTSHSSFTLNGNNSVAIILTIISYLMLIFY
NHLLVYLKPFFLPKIM

Sequences:

>Translated_496_residues
MSNIFQAVKDHFVILEVLIPFFGSLIVVLYKKVEYALIVSRLCAILGIILGIYGVLSMINSPPYFYAIGNWQAPIGIEYR
VDHVNQIIIVFSYFILFLLLTFNSQLIKTNLLSSLNPSRSHLLYSILLLLHTGFCGIILSNDLFNIYVFIEIASLASYTL
VSQGVDKRALIGSINYLILGTIGATFILIAIGFILSNTGSLNITDIISRLPQQHTKTLIAAIMFYITGCLLKVAMFPMSS
WLISTYKYTSSVILSYFAPISCMVGFYILLYFVYQIIGITNINTLHSYYIINALGLAGAIFCSCYAVQQSNLKSIMAYSV
LAETSYISLMAFNIQSSTIGAQIIVILLTSCILKSIIYSLISRIEVHYHDSELKHIAGIGSTYPVFGILFTLALISNLGL
PLTIGFVNKLNIFLTVLQSLGYFGIIILAISSCMSFNYHYKIFHYLYSTQQTSHSSFTLNGNNSVAIILTIISYLMLIFY
NHLLVYLKPFFLPKIM
>Mature_495_residues
SNIFQAVKDHFVILEVLIPFFGSLIVVLYKKVEYALIVSRLCAILGIILGIYGVLSMINSPPYFYAIGNWQAPIGIEYRV
DHVNQIIIVFSYFILFLLLTFNSQLIKTNLLSSLNPSRSHLLYSILLLLHTGFCGIILSNDLFNIYVFIEIASLASYTLV
SQGVDKRALIGSINYLILGTIGATFILIAIGFILSNTGSLNITDIISRLPQQHTKTLIAAIMFYITGCLLKVAMFPMSSW
LISTYKYTSSVILSYFAPISCMVGFYILLYFVYQIIGITNINTLHSYYIINALGLAGAIFCSCYAVQQSNLKSIMAYSVL
AETSYISLMAFNIQSSTIGAQIIVILLTSCILKSIIYSLISRIEVHYHDSELKHIAGIGSTYPVFGILFTLALISNLGLP
LTIGFVNKLNIFLTVLQSLGYFGIIILAISSCMSFNYHYKIFHYLYSTQQTSHSSFTLNGNNSVAIILTIISYLMLIFYN
HLLVYLKPFFLPKIM

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=364, Percent_Identity=21.4285714285714, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1788829, Length=495, Percent_Identity=22.4242424242424, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55434; Mature: 55303

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIFQAVKDHFVILEVLIPFFGSLIVVLYKKVEYALIVSRLCAILGIILGIYGVLSMIN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPPYFYAIGNWQAPIGIEYRVDHVNQIIIVFSYFILFLLLTFNSQLIKTNLLSSLNPSRS
CCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHH
HLLYSILLLLHTGFCGIILSNDLFNIYVFIEIASLASYTLVSQGVDKRALIGSINYLILG
HHHHHHHHHHHCCHHHEEECCCCEEEEHEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TIGATFILIAIGFILSNTGSLNITDIISRLPQQHTKTLIAAIMFYITGCLLKVAMFPMSS
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLISTYKYTSSVILSYFAPISCMVGFYILLYFVYQIIGITNINTLHSYYIINALGLAGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FCSCYAVQQSNLKSIMAYSVLAETSYISLMAFNIQSSTIGAQIIVILLTSCILKSIIYSL
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ISRIEVHYHDSELKHIAGIGSTYPVFGILFTLALISNLGLPLTIGFVNKLNIFLTVLQSL
HHHHHHEECHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
GYFGIIILAISSCMSFNYHYKIFHYLYSTQQTSHSSFTLNGNNSVAIILTIISYLMLIFY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
NHLLVYLKPFFLPKIM
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNIFQAVKDHFVILEVLIPFFGSLIVVLYKKVEYALIVSRLCAILGIILGIYGVLSMIN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SPPYFYAIGNWQAPIGIEYRVDHVNQIIIVFSYFILFLLLTFNSQLIKTNLLSSLNPSRS
CCCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHH
HLLYSILLLLHTGFCGIILSNDLFNIYVFIEIASLASYTLVSQGVDKRALIGSINYLILG
HHHHHHHHHHHCCHHHEEECCCCEEEEHEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TIGATFILIAIGFILSNTGSLNITDIISRLPQQHTKTLIAAIMFYITGCLLKVAMFPMSS
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WLISTYKYTSSVILSYFAPISCMVGFYILLYFVYQIIGITNINTLHSYYIINALGLAGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FCSCYAVQQSNLKSIMAYSVLAETSYISLMAFNIQSSTIGAQIIVILLTSCILKSIIYSL
HHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
ISRIEVHYHDSELKHIAGIGSTYPVFGILFTLALISNLGLPLTIGFVNKLNIFLTVLQSL
HHHHHHEECHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHH
GYFGIIILAISSCMSFNYHYKIFHYLYSTQQTSHSSFTLNGNNSVAIILTIISYLMLIFY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHH
NHLLVYLKPFFLPKIM
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA