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The map label for this gene is gatA

Identifier: 148284746

GI number: 148284746

Start: 1327950

End: 1329428

Strand: Direct

Name: gatA

Synonym: OTBS_1367

Alternate gene names: 148284746

Gene position: 1327950-1329428 (Clockwise)

Preceding gene: 148284745

Following gene: 148284747

Centisome position: 62.43

GC content: 34.01

Gene sequence:

>1479_bases
ATGAACAATCTAGTAAAATTAACAATTTCTCAAGCAGTTAGTGGGCTAAAAAATAAAGAGTTTACTGCTACTGATCTAGT
AACTGCGCATATCAACCAAATGGATAAGTATAGGTATATTAATTCTTATATTACTGAGATACCAGAACTTGCTTTACAGG
CAGCTAAAAAATCTGATTTTTTAATTCAAAATAATCAAGCTCGGCCAATTGAAGGTATTCCAGTTTCTATTAAGGATCTT
TTCTGTACTAAAGGAATATTAACTACTGCTGCATCAAAGATGCTACAAAACTTTACTCCTGTTTACGATGCTACTGCATA
TAGTAAAATCATTAATGCTGGCGGCATTATGCTAGGAAAAGGTAATATGGATGAATTTGCTATGGGATCAGCAAACATTA
ATAGTTATTTTGGTAATGTAATTAATCCCTGGAAGGCTGAAAGTGATGATGTAGATTTGACTCCTGGTGGATCTTCAGGT
GGATCTTCAGCTGCCGTTGCAGCATTTATGGCAATGGCAGCATTAGGTAGTGATACTGGAGGATCTGTTAGGCAACCAGC
ATCTTATACTGGAACTGTTGGTATAAAGCCAAGTTATGGACGATGCTCAAGATGGGGTATGATTGCATTTTCATGCTCGC
TGGATCAACCTGGAGTAATTACTCGTACAGTTGAAGATGCAGCAATTATGCTAGAAATCATGATGGGATATGATGAGAAG
GACTCGACAAGCTTAAATGCAGAAGTTCCTAACCTAAGATCAGCTGTTAATCAACCTATTAAAGGTATGAAAATAGGTAT
ACCTTATGATTTAATGGAAAATAGGAGCTTAAGTAGTGAAATTATAACAATGTGGCAAAACACTATAAATTTGTTAAAAG
ATCATGGTGTAGAAATTGTATCTATTGGCTTGCCATATATTAATTATGCATTACCAGTATATTATGTGCTTTCTTCTGCT
GAAGCTTCATCAAATTTAGCAAGATATGATGGAGTAAGATATGGGTTAAGGGTAGAGGGAAAAGATTCAAATATTAATGA
AATTTATGAACTTTCCAGATCAGAAGGATTTGGAGCTGAAGTTAAAAGAAGAATTATGATGGGTACATATGCACTTGCAT
CAACAAATATCAATTCGTATTATATTAAAGCTCAACAAGTAAGACGATTAATAGTGAATGACTTCACAAATAGTTTTAAT
AAAGTAGATTGTATATTAATTCCATCTGCGCCTACTGCAGCTTTTCCACTTAACTCTAATCAAGATGATCCAGTTACTAT
GTATTTAAATGATATATTGACAATACCAGCAAGTTTAGCTGGGTTGCCATGCATTTCAATACCAGCTGGGTTTTCAGCTA
ACAAGTTGCCATTAGGTATGCAAGTTATTGGTTCTCGCTTAGATGAGTATAATATTATTAAAATATCATCAGCTATTGAA
AAAGCACTCAATCTCAAATTTATTCCAAAAGGTTTTTAA

Upstream 100 bases:

>100_bases
AGAATATTACAGTGGATCAGTTATTATCTAATGTACCACAGTCATCAGCAACTATAGCTAAAAACACAAAATATTTTGTT
GTACCAAAGATAATAGAATA

Downstream 100 bases:

>100_bases
ATATGACATTTATAGAAGGCAAAACTGAAAAATGGGAATATGTTATAGGACTTGAAATTCATGCTCAAATAAAATCCAAT
GCTAAGTTATTTTCTAGTGC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNNLVKLTISQAVSGLKNKEFTATDLVTAHINQMDKYRYINSYITEIPELALQAAKKSDFLIQNNQARPIEGIPVSIKDL
FCTKGILTTAASKMLQNFTPVYDATAYSKIINAGGIMLGKGNMDEFAMGSANINSYFGNVINPWKAESDDVDLTPGGSSG
GSSAAVAAFMAMAALGSDTGGSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQPGVITRTVEDAAIMLEIMMGYDEK
DSTSLNAEVPNLRSAVNQPIKGMKIGIPYDLMENRSLSSEIITMWQNTINLLKDHGVEIVSIGLPYINYALPVYYVLSSA
EASSNLARYDGVRYGLRVEGKDSNINEIYELSRSEGFGAEVKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFN
KVDCILIPSAPTAAFPLNSNQDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
KALNLKFIPKGF

Sequences:

>Translated_492_residues
MNNLVKLTISQAVSGLKNKEFTATDLVTAHINQMDKYRYINSYITEIPELALQAAKKSDFLIQNNQARPIEGIPVSIKDL
FCTKGILTTAASKMLQNFTPVYDATAYSKIINAGGIMLGKGNMDEFAMGSANINSYFGNVINPWKAESDDVDLTPGGSSG
GSSAAVAAFMAMAALGSDTGGSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQPGVITRTVEDAAIMLEIMMGYDEK
DSTSLNAEVPNLRSAVNQPIKGMKIGIPYDLMENRSLSSEIITMWQNTINLLKDHGVEIVSIGLPYINYALPVYYVLSSA
EASSNLARYDGVRYGLRVEGKDSNINEIYELSRSEGFGAEVKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFN
KVDCILIPSAPTAAFPLNSNQDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
KALNLKFIPKGF
>Mature_492_residues
MNNLVKLTISQAVSGLKNKEFTATDLVTAHINQMDKYRYINSYITEIPELALQAAKKSDFLIQNNQARPIEGIPVSIKDL
FCTKGILTTAASKMLQNFTPVYDATAYSKIINAGGIMLGKGNMDEFAMGSANINSYFGNVINPWKAESDDVDLTPGGSSG
GSSAAVAAFMAMAALGSDTGGSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQPGVITRTVEDAAIMLEIMMGYDEK
DSTSLNAEVPNLRSAVNQPIKGMKIGIPYDLMENRSLSSEIITMWQNTINLLKDHGVEIVSIGLPYINYALPVYYVLSSA
EASSNLARYDGVRYGLRVEGKDSNINEIYELSRSEGFGAEVKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFN
KVDCILIPSAPTAAFPLNSNQDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
KALNLKFIPKGF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=508, Percent_Identity=38.3858267716535, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI195972892, Length=519, Percent_Identity=23.5067437379576, Blast_Score=121, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17543272, Length=457, Percent_Identity=37.636761487965, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17537465, Length=516, Percent_Identity=24.8062015503876, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=243, Percent_Identity=30.4526748971193, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=225, Percent_Identity=28.4444444444444, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=211, Percent_Identity=27.4881516587678, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=36.2997658079625, Blast_Score=252, Evalue=7e-68,
Organism=Saccharomyces cerevisiae, GI6319685, Length=428, Percent_Identity=25, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648113, Length=495, Percent_Identity=36.7676767676768, Blast_Score=298, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=24.3137254901961, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI161078093, Length=519, Percent_Identity=24.8554913294798, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652985, Length=492, Percent_Identity=24.5934959349593, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI19922090, Length=492, Percent_Identity=24.5934959349593, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24652981, Length=492, Percent_Identity=24.5934959349593, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24652983, Length=492, Percent_Identity=24.5934959349593, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI45550774, Length=195, Percent_Identity=30.7692307692308, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648435, Length=195, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648437, Length=195, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648441, Length=130, Percent_Identity=35.3846153846154, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648439, Length=130, Percent_Identity=35.3846153846154, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356731, Length=495, Percent_Identity=21.6161616161616, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_ORITB (A5CEC8)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248836.1
- ProteinModelPortal:   A5CEC8
- SMR:   A5CEC8
- STRING:   A5CEC8
- GeneID:   5217744
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1367
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   OTSU357244:OTBS_1367-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 53405; Mature: 53405

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLVKLTISQAVSGLKNKEFTATDLVTAHINQMDKYRYINSYITEIPELALQAAKKSDF
CCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LIQNNQARPIEGIPVSIKDLFCTKGILTTAASKMLQNFTPVYDATAYSKIINAGGIMLGK
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEC
GNMDEFAMGSANINSYFGNVINPWKAESDDVDLTPGGSSGGSSAAVAAFMAMAALGSDTG
CCCCHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
GSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQPGVITRTVEDAAIMLEIMMGYDEK
CCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCCC
DSTSLNAEVPNLRSAVNQPIKGMKIGIPYDLMENRSLSSEIITMWQNTINLLKDHGVEIV
CCCCCCCCCCHHHHHHCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
SIGLPYINYALPVYYVLSSAEASSNLARYDGVRYGLRVEGKDSNINEIYELSRSEGFGAE
EECCCHHHHHHHHHHHHHCCHHHCCHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCCCHH
VKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFNKVDCILIPSAPTAAFPLNSN
HHHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCC
QDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
CCCCEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEEHHHHH
KALNLKFIPKGF
HHHCCCCCCCCC
>Mature Secondary Structure
MNNLVKLTISQAVSGLKNKEFTATDLVTAHINQMDKYRYINSYITEIPELALQAAKKSDF
CCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
LIQNNQARPIEGIPVSIKDLFCTKGILTTAASKMLQNFTPVYDATAYSKIINAGGIMLGK
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEC
GNMDEFAMGSANINSYFGNVINPWKAESDDVDLTPGGSSGGSSAAVAAFMAMAALGSDTG
CCCCHHCCCCCCHHHHHCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCCCC
GSVRQPASYTGTVGIKPSYGRCSRWGMIAFSCSLDQPGVITRTVEDAAIMLEIMMGYDEK
CCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHCCCCC
DSTSLNAEVPNLRSAVNQPIKGMKIGIPYDLMENRSLSSEIITMWQNTINLLKDHGVEIV
CCCCCCCCCCHHHHHHCCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEE
SIGLPYINYALPVYYVLSSAEASSNLARYDGVRYGLRVEGKDSNINEIYELSRSEGFGAE
EECCCHHHHHHHHHHHHHCCHHHCCHHHHCCEEEEEEEECCCCCHHHHHHHHCCCCCCHH
VKRRIMMGTYALASTNINSYYIKAQQVRRLIVNDFTNSFNKVDCILIPSAPTAAFPLNSN
HHHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCC
QDDPVTMYLNDILTIPASLAGLPCISIPAGFSANKLPLGMQVIGSRLDEYNIIKISSAIE
CCCCEEEEEHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHHHHCCCCCCEEEEEHHHHH
KALNLKFIPKGF
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA