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The map label for this gene is gatB

Identifier: 148284747

GI number: 148284747

Start: 1329431

End: 1330891

Strand: Direct

Name: gatB

Synonym: OTBS_1368

Alternate gene names: 148284747

Gene position: 1329431-1330891 (Clockwise)

Preceding gene: 148284746

Following gene: 148284748

Centisome position: 62.5

GC content: 30.66

Gene sequence:

>1461_bases
ATGACATTTATAGAAGGCAAAACTGAAAAATGGGAATATGTTATAGGACTTGAAATTCATGCTCAAATAAAATCCAATGC
TAAGTTATTTTCTAGTGCTTCAACTGAATTTGGAAGTTCTCCAAACTCACAGGTCGAATTGTTAGATGCAGCTATGCCAG
GATCTTTACCAGTATTAAATGAATTTTGTGTGCATCAAGCTATAAAAACTGCTCTAGGAATTAATGCGAAAATAAATAAA
TTATCTATTTTTGACCGTAAAAACTACTTTTATGCTGATTTACCAGCAGGTTATCAAATTTCGCAATTTTATCATCCAAT
TGCTCAAGGCGGTTGGATAGAAATATTAGATGAAAATGGTAATATTAAGTGCATTCAGATTAATAGGTTGCATATAGAAC
AAGATACAGGAAAAAGTACCCACGATCAATCGGACACTTATAGCTATATAGATCTTAATCGAAGTGGTATAGCATTAATG
GAGATAGTTTCTGAACCTGATATTTCTTCTCCTATGCAAGCAGCCGAATATATCAAAAAACTTAGAGCAATATTGAGATA
TTTAGATAGTTGTAATGGTGATATGGAAAAAGGGGCTCTAAGATGTGATGCTAATATATCAGTAAGAAAGCCAAATAGTG
AATTAGGAACTAAATGTGAAATTAAGAACCTTAATTCAATTAAATCAATTGTTAGAGCACTAGAATTTGAAGGTCAAAGG
CAGGTCAATATATTAGAATCTGGTGGTACAGTTAAACAGGAAAGTCTTCTATTTGATGCTACACTAGGCAAAACATTTCC
AATGAGATCTAAAGAGAATGCTACCGATTATAGATATTTTCCTGATCCAGACCTACCTCCAATAATATTAGATCAGTCAT
TAATTGACAATATTGCTAGTAGTTTACCCGAATTACCAGATGCTAAAATTACAAGATATGTTAATGAAATCAAGCTTAGT
GATTATAATGCTCAAGTTTTAGCAGCTGATAAGGATATTTCCTGCTTTTTTGAAGAAGTTATTAAAACTGCAAATCCTAT
ATTAACTGCTAACTGGATATTAAGTGAGTTATTTGGATTAATGAATAAAGATGGTATTACTATTAACGAATGTAAAATTA
CTGCTAATCACTTTTCTGAATTAATACAGTTGATTAGTTCCAAAGCAATATCTAGCAAAATTGCAAAAACAGTCTTAAAA
GAAATGTTTGATAGTGGAAAGAGTCCAAAGATAATTATGCAAGAAAAAAATATACAGCAAATTTCAGATCCTAATCAAAT
TGCTGATATTATTGATGATGTACTTAAGGATAATTATCAGTCTGTTGTTTCCTATAGAAATGGTAAAGACAGATTATTTG
GATTTTTTGTTGGCCAAGTAATGAAGAAAACTGCTGGTAATGCAAATCCTGAGCTTATCAATGAAATATTGCATACAAAA
TTAAAACAATTTCAAATTTAA

Upstream 100 bases:

>100_bases
AAGTTATTGGTTCTCGCTTAGATGAGTATAATATTATTAAAATATCATCAGCTATTGAAAAAGCACTCAATCTCAAATTT
ATTCCAAAAGGTTTTTAAAT

Downstream 100 bases:

>100_bases
ATTTGTTTTCTAATCTAATAAAAGTCTAACATCAATAAAACAGTTTATTTGTTTTGATTTTTTATACAATCTCTTATCCA
TATATTCAGCAATTACCTCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLNEFCVHQAIKTALGINAKINK
LSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENGNIKCIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALM
EIVSEPDISSPMQAAEYIKKLRAILRYLDSCNGDMEKGALRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIASSLPELPDAKITRYVNEIKLS
DYNAQVLAADKDISCFFEEVIKTANPILTANWILSELFGLMNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLK
EMFDSGKSPKIIMQEKNIQQISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPELINEILHTK
LKQFQI

Sequences:

>Translated_486_residues
MTFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLNEFCVHQAIKTALGINAKINK
LSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENGNIKCIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALM
EIVSEPDISSPMQAAEYIKKLRAILRYLDSCNGDMEKGALRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIASSLPELPDAKITRYVNEIKLS
DYNAQVLAADKDISCFFEEVIKTANPILTANWILSELFGLMNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLK
EMFDSGKSPKIIMQEKNIQQISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPELINEILHTK
LKQFQI
>Mature_485_residues
TFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLNEFCVHQAIKTALGINAKINKL
SIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENGNIKCIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALME
IVSEPDISSPMQAAEYIKKLRAILRYLDSCNGDMEKGALRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQRQ
VNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIASSLPELPDAKITRYVNEIKLSD
YNAQVLAADKDISCFFEEVIKTANPILTANWILSELFGLMNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLKE
MFDSGKSPKIIMQEKNIQQISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPELINEILHTKL
KQFQI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=39.8785425101215, Blast_Score=358, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI115534094, Length=466, Percent_Identity=32.8326180257511, Blast_Score=230, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=32.6923076923077, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45550808, Length=492, Percent_Identity=34.1463414634146, Blast_Score=308, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_ORITB (A5CEC9)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001248837.1
- ProteinModelPortal:   A5CEC9
- SMR:   A5CEC9
- STRING:   A5CEC9
- GeneID:   5217745
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_1368
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   OTSU357244:OTBS_1368-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54429; Mature: 54298

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLN
CCCCCCCCCCEEEEEEEEEEEEECCCHHHHHCCCHHCCCCCCCCEEEEECCCCCCCHHHH
EFCVHQAIKTALGINAKINKLSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENG
HHHHHHHHHHHHCCCCEECEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCC
NIKCIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALMEIVSEPDISSPMQAAEYIKK
CEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHH
LRAILRYLDSCNGDMEKGALRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
HHHHHHHHHHCCCCCCCCCEEECCCEEEECCCHHHCCEEEECCHHHHHHHHHHHHCCCCC
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIAS
EEEEEECCCCEEHHHEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHH
SLPELPDAKITRYVNEIKLSDYNAQVLAADKDISCFFEEVIKTANPILTANWILSELFGL
HCCCCCCHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHHHH
MNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLKEMFDSGKSPKIIMQEKNIQQ
HCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
ISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPELINEILHTK
CCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LKQFQI
HHHCCC
>Mature Secondary Structure 
TFIEGKTEKWEYVIGLEIHAQIKSNAKLFSSASTEFGSSPNSQVELLDAAMPGSLPVLN
CCCCCCCCCEEEEEEEEEEEEECCCHHHHHCCCHHCCCCCCCCEEEEECCCCCCCHHHH
EFCVHQAIKTALGINAKINKLSIFDRKNYFYADLPAGYQISQFYHPIAQGGWIEILDENG
HHHHHHHHHHHHCCCCEECEEEEEECCCCEEEECCCCCCHHHHHHHHHCCCEEEEEECCC
NIKCIQINRLHIEQDTGKSTHDQSDTYSYIDLNRSGIALMEIVSEPDISSPMQAAEYIKK
CEEEEEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCHHHHHHHHHH
LRAILRYLDSCNGDMEKGALRCDANISVRKPNSELGTKCEIKNLNSIKSIVRALEFEGQR
HHHHHHHHHHCCCCCCCCCEEECCCEEEECCCHHHCCEEEECCHHHHHHHHHHHHCCCCC
QVNILESGGTVKQESLLFDATLGKTFPMRSKENATDYRYFPDPDLPPIILDQSLIDNIAS
EEEEEECCCCEEHHHEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHH
SLPELPDAKITRYVNEIKLSDYNAQVLAADKDISCFFEEVIKTANPILTANWILSELFGL
HCCCCCCHHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHHHHCCCCEEHHHHHHHHHHH
MNKDGITINECKITANHFSELIQLISSKAISSKIAKTVLKEMFDSGKSPKIIMQEKNIQQ
HCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
ISDPNQIADIIDDVLKDNYQSVVSYRNGKDRLFGFFVGQVMKKTAGNANPELINEILHTK
CCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LKQFQI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA