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The map label for this gene is gluQ [H]

Identifier: 148260328

GI number: 148260328

Start: 1482178

End: 1483023

Strand: Reverse

Name: gluQ [H]

Synonym: Acry_1325

Alternate gene names: 148260328

Gene position: 1483023-1482178 (Counterclockwise)

Preceding gene: 148260333

Following gene: 148260327

Centisome position: 43.76

GC content: 73.88

Gene sequence:

>846_bases
ATGTCCATCGTCACCCGTTTCGCGCCGAGCCCGACCGGCTACCTGCATCTCGGCCACGCGGCCTCGGCCATGGCCGGGCA
CGACCTGGCGCGCGGGGCGGGCGGGCGGTTCCTGCTGCGGCTCGAGGATATCGATGCCGGGCGCTGCCGGCCCGGATTCG
CCGAGGCGATCCTGGAGGACCTGGCCTGGCTCGGCCTGGCCTGGGATGGCGCGGTGCGGGTGCAGTCGGCGCATTTCGAC
GAGTACCGGGCGGCGCTGGCGCGGCTGGAGGCGATGGGATTGCTCTACCGGTGCTTCTGCACGCGGGCGGCGATTGCCGC
GGCCCTCGGCGCGCCGCACGGGGCCGAGGCGGCCTATCCCGGCACCTGCCGCGGGCTCGATCCGGCGCTGGCGGACGCGC
GGGCGGCGCAAGGCGAGCCCTATGCGCTGCGGCTGCGGATGGACCGGGCCGCGGCGATGGCCGGGCGGCTGCGCTATCAC
GAGGCGGCGGAAGGCTGGGTGGAGGCCGATCCGCGGCCGCATGGCGATGTGGTGCTGGCGCGCAAGGACGTGCCGGCGAG
CTATCATCTCTGCGTCTGCCATGACGATGCGGCGCAGGAGGTGACGCATGTGACGCGGGGCGAGGACCTGCGGGCGACGA
CGGATGTTCACGTGCTGCTGCAGGCGCTGCTTGGCTGGCCGCATCCGGCCTATGCCCATCATCCGCTGATCCGCGACGCG
GCGGGGCGGCGGCTGGCCAAGCGCGACCGGGCGGCGACGCTGCGCGATCTGCGGGACGCCGGGGCCACGCCCGCGCAAAT
CCGCGAGATGGTCTCTTGCCGGATCGGCGATCTCGCCTCACTCTGA

Upstream 100 bases:

>100_bases
CGCCTTGCCGCCGTTCGCCGGGCCCCAGCGCGGTCCGTCCAGTTCAGCCATGAAGTCCTGCTCCTTGCATTTGCGGGGGA
GCGTATCATCGTGGAGGCCG

Downstream 100 bases:

>100_bases
CAGGATGATGCGTTTCGCGATTTTGGTTCCGACCGCCGGCGCGGCCCTCGCGCTCGGCCTGCTGCTGTCCTCGTCCCCGG
CCCATGCCGTGCCGGCGCCG

Product: glutamyl-tRNA synthetase, class Ic

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSIVTRFAPSPTGYLHLGHAASAMAGHDLARGAGGRFLLRLEDIDAGRCRPGFAEAILEDLAWLGLAWDGAVRVQSAHFD
EYRAALARLEAMGLLYRCFCTRAAIAAALGAPHGAEAAYPGTCRGLDPALADARAAQGEPYALRLRMDRAAAMAGRLRYH
EAAEGWVEADPRPHGDVVLARKDVPASYHLCVCHDDAAQEVTHVTRGEDLRATTDVHVLLQALLGWPHPAYAHHPLIRDA
AGRRLAKRDRAATLRDLRDAGATPAQIREMVSCRIGDLASL

Sequences:

>Translated_281_residues
MSIVTRFAPSPTGYLHLGHAASAMAGHDLARGAGGRFLLRLEDIDAGRCRPGFAEAILEDLAWLGLAWDGAVRVQSAHFD
EYRAALARLEAMGLLYRCFCTRAAIAAALGAPHGAEAAYPGTCRGLDPALADARAAQGEPYALRLRMDRAAAMAGRLRYH
EAAEGWVEADPRPHGDVVLARKDVPASYHLCVCHDDAAQEVTHVTRGEDLRATTDVHVLLQALLGWPHPAYAHHPLIRDA
AGRRLAKRDRAATLRDLRDAGATPAQIREMVSCRIGDLASL
>Mature_280_residues
SIVTRFAPSPTGYLHLGHAASAMAGHDLARGAGGRFLLRLEDIDAGRCRPGFAEAILEDLAWLGLAWDGAVRVQSAHFDE
YRAALARLEAMGLLYRCFCTRAAIAAALGAPHGAEAAYPGTCRGLDPALADARAAQGEPYALRLRMDRAAAMAGRLRYHE
AAEGWVEADPRPHGDVVLARKDVPASYHLCVCHDDAAQEVTHVTRGEDLRATTDVHVLLQALLGWPHPAYAHHPLIRDAA
GRRLAKRDRAATLRDLRDAGATPAQIREMVSCRIGDLASL

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=286, Percent_Identity=31.4685314685315, Blast_Score=131, Evalue=7e-31,
Organism=Escherichia coli, GI1788743, Length=266, Percent_Identity=34.9624060150376, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI226510925, Length=252, Percent_Identity=38.0952380952381, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1786895, Length=95, Percent_Identity=37.8947368421053, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=266, Percent_Identity=31.203007518797, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324540, Length=269, Percent_Identity=32.3420074349442, Blast_Score=152, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321192, Length=80, Percent_Identity=37.5, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24665321, Length=280, Percent_Identity=33.5714285714286, Blast_Score=150, Evalue=8e-37,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 30284; Mature: 30153

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00334 MYB_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVTRFAPSPTGYLHLGHAASAMAGHDLARGAGGRFLLRLEDIDAGRCRPGFAEAILED
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
LAWLGLAWDGAVRVQSAHFDEYRAALARLEAMGLLYRCFCTRAAIAAALGAPHGAEAAYP
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GTCRGLDPALADARAAQGEPYALRLRMDRAAAMAGRLRYHEAAEGWVEADPRPHGDVVLA
CCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
RKDVPASYHLCVCHDDAAQEVTHVTRGEDLRATTDVHVLLQALLGWPHPAYAHHPLIRDA
ECCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCHHHHH
AGRRLAKRDRAATLRDLRDAGATPAQIREMVSCRIGDLASL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHCCC
>Mature Secondary Structure 
SIVTRFAPSPTGYLHLGHAASAMAGHDLARGAGGRFLLRLEDIDAGRCRPGFAEAILED
CCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
LAWLGLAWDGAVRVQSAHFDEYRAALARLEAMGLLYRCFCTRAAIAAALGAPHGAEAAYP
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GTCRGLDPALADARAAQGEPYALRLRMDRAAAMAGRLRYHEAAEGWVEADPRPHGDVVLA
CCCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
RKDVPASYHLCVCHDDAAQEVTHVTRGEDLRATTDVHVLLQALLGWPHPAYAHHPLIRDA
ECCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCHHHHH
AGRRLAKRDRAATLRDLRDAGATPAQIREMVSCRIGDLASL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA