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The map label for this gene is purD [H]

Identifier: 148260333

GI number: 148260333

Start: 1486669

End: 1487934

Strand: Reverse

Name: purD [H]

Synonym: Acry_1330

Alternate gene names: 148260333

Gene position: 1487934-1486669 (Counterclockwise)

Preceding gene: 148260335

Following gene: 148260328

Centisome position: 43.9

GC content: 73.85

Gene sequence:

>1266_bases
ATGCGGGTTCTGGTTGTCGGCGGCGGCGGGCGCGAGCATGCGCTGGCGTGGAAGCTCGCGCAGAGCCCGGTGCTGGAGAC
GCTCTTCGTCGCGCCGGGGAATCCGGGCATGGAATCGCTGGCGAAATGCGTCGGGATCGGCGCCGAGGAGATCGACGCGC
TCGTGGAGTTCGCGCGGGCGGAGCGGATCGACCTCGTGGTGCCGGGGCCGGAGGCGCCGCTGGCGCTCGGCCTCGCGGAC
CGGCTGGCGGCGGCGGGAATCGCCTGTTTCGGCCCGACCGAGGCGGCGGCGCGGCTCGAATCCTCGAAGCGGTTCACCAA
GGAGATCTGCGACGAGGCGGGGATTCCGACGGCCGGCTGGGCCAGCTTCACCGATGCCGGCGCGGCGCGCGCCTATCTCG
ACGAGCATCCGGCGCCGATCGTGATCAAGGCGGACGGGCTGGCGGCGGGCAAGGGCGTGGTCGTCGCCGCGACCGATGAG
GAGGCGCGCGAGGCCGTGGCGGCGATGCTGGAGGGCGGGCTGCACGGCGCCTCGGGTGCGGAGATCGTGATCGAGGAATG
CCTGATCGGCACCGAGATCTCGCTGTTCGCGATCTGCGACGGCGAGGCCGCGGTGTTCTGCGGCACCGCCTCGGACCACA
AGCGGGTGGGCGATGGCGATACCGGGCCGAATACCGGCGGGATGGGCGCGATCGCGCCGCCGCCGGCGGCCTCGGGGGCG
GTGATCGGGGCGGCGATGGAGCGGATCGTCCGCCCGGCGCTGCGGACGATGGCGCGGCGCGGGACGCCGTTCCGCGGCTT
CCTGTTCGCCGGGCTGATGCTCACCGCCTCCGGCCCCCGGCTGATCGAGTTCAACGTGCGGTTCGGCGATCCGGAATGCG
AGACGCTGCTGCCGCTGCTGAGGACCGACCTGTTGCCGGTGCTCGCCGATGCGGCGCGGGGGCGGCTCGATCCGGAAACC
CGGCTCGACTGGCGGGACGGCGCGGCGGCGACGGTGGTGATGGCGGCGAAGGGCTATCCGGGCGCCTATGAGAGGGGCCA
TCCGATCGACCTCGCCGGGGTGGCCGGGCTGCCGGAGACGACGGTGTTCCAGGCCGGCACGGCGCGGCGCGACGGCGCGC
TGGTGAGTGCCGGCGGGCGGGTGCTGGCGGTGACGGCGACGGGCGATGACCTGCGCGAGGCGATCGACCGCGCCTATGTC
GCGGTGCGGCGGATCGGCTTTCCCGGCGGGTTCTGCCGCCGGGATATCGGCGCCAAGGCGGTGTAG

Upstream 100 bases:

>100_bases
TGAGTTCGGGCAGGTTGCCGGTGGCGGTGTCCGTGGTTTCGCTCATGGCGGCGGACTATGCTAAGCGCGCGCCGGTTGCA
CAAGCGCAAGGGGGTCGATC

Downstream 100 bases:

>100_bases
TGCCCTGCCCCTGAAATTCGTGGGTGAATTTCAGGGACAGGCGGGCCACCAAACTATTGTTTTTTTATCAATACGATAGA
GCACTACATCCGATCCGGAA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MRVLVVGGGGREHALAWKLAQSPVLETLFVAPGNPGMESLAKCVGIGAEEIDALVEFARAERIDLVVPGPEAPLALGLAD
RLAAAGIACFGPTEAAARLESSKRFTKEICDEAGIPTAGWASFTDAGAARAYLDEHPAPIVIKADGLAAGKGVVVAATDE
EAREAVAAMLEGGLHGASGAEIVIEECLIGTEISLFAICDGEAAVFCGTASDHKRVGDGDTGPNTGGMGAIAPPPAASGA
VIGAAMERIVRPALRTMARRGTPFRGFLFAGLMLTASGPRLIEFNVRFGDPECETLLPLLRTDLLPVLADAARGRLDPET
RLDWRDGAAATVVMAAKGYPGAYERGHPIDLAGVAGLPETTVFQAGTARRDGALVSAGGRVLAVTATGDDLREAIDRAYV
AVRRIGFPGGFCRRDIGAKAV

Sequences:

>Translated_421_residues
MRVLVVGGGGREHALAWKLAQSPVLETLFVAPGNPGMESLAKCVGIGAEEIDALVEFARAERIDLVVPGPEAPLALGLAD
RLAAAGIACFGPTEAAARLESSKRFTKEICDEAGIPTAGWASFTDAGAARAYLDEHPAPIVIKADGLAAGKGVVVAATDE
EAREAVAAMLEGGLHGASGAEIVIEECLIGTEISLFAICDGEAAVFCGTASDHKRVGDGDTGPNTGGMGAIAPPPAASGA
VIGAAMERIVRPALRTMARRGTPFRGFLFAGLMLTASGPRLIEFNVRFGDPECETLLPLLRTDLLPVLADAARGRLDPET
RLDWRDGAAATVVMAAKGYPGAYERGHPIDLAGVAGLPETTVFQAGTARRDGALVSAGGRVLAVTATGDDLREAIDRAYV
AVRRIGFPGGFCRRDIGAKAV
>Mature_421_residues
MRVLVVGGGGREHALAWKLAQSPVLETLFVAPGNPGMESLAKCVGIGAEEIDALVEFARAERIDLVVPGPEAPLALGLAD
RLAAAGIACFGPTEAAARLESSKRFTKEICDEAGIPTAGWASFTDAGAARAYLDEHPAPIVIKADGLAAGKGVVVAATDE
EAREAVAAMLEGGLHGASGAEIVIEECLIGTEISLFAICDGEAAVFCGTASDHKRVGDGDTGPNTGGMGAIAPPPAASGA
VIGAAMERIVRPALRTMARRGTPFRGFLFAGLMLTASGPRLIEFNVRFGDPECETLLPLLRTDLLPVLADAARGRLDPET
RLDWRDGAAATVVMAAKGYPGAYERGHPIDLAGVAGLPETTVFQAGTARRDGALVSAGGRVLAVTATGDDLREAIDRAYV
AVRRIGFPGGFCRRDIGAKAV

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=45.5607476635514, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=45.5607476635514, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=45.5607476635514, Blast_Score=361, Evalue=1e-100,
Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=45.5607476635514, Blast_Score=361, Evalue=1e-100,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=51.1682242990654, Blast_Score=386, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17567511, Length=424, Percent_Identity=37.9716981132075, Blast_Score=272, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=41.7977528089888, Blast_Score=316, Evalue=4e-87,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=43.1235431235431, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24582400, Length=429, Percent_Identity=43.1235431235431, Blast_Score=307, Evalue=9e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 43311; Mature: 43311

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVVGGGGREHALAWKLAQSPVLETLFVAPGNPGMESLAKCVGIGAEEIDALVEFARA
CEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
ERIDLVVPGPEAPLALGLADRLAAAGIACFGPTEAAARLESSKRFTKEICDEAGIPTAGW
CCEEEEECCCCCCEEHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASFTDAGAARAYLDEHPAPIVIKADGLAAGKGVVVAATDEEAREAVAAMLEGGLHGASGA
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCH
EIVIEECLIGTEISLFAICDGEAAVFCGTASDHKRVGDGDTGPNTGGMGAIAPPPAASGA
HHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
VIGAAMERIVRPALRTMARRGTPFRGFLFAGLMLTASGPRLIEFNVRFGDPECETLLPLL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHH
RTDLLPVLADAARGRLDPETRLDWRDGAAATVVMAAKGYPGAYERGHPIDLAGVAGLPET
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
TVFQAGTARRDGALVSAGGRVLAVTATGDDLREAIDRAYVAVRRIGFPGGFCRRDIGAKA
CEEECCCCCCCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCC
V
C
>Mature Secondary Structure
MRVLVVGGGGREHALAWKLAQSPVLETLFVAPGNPGMESLAKCVGIGAEEIDALVEFARA
CEEEEECCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
ERIDLVVPGPEAPLALGLADRLAAAGIACFGPTEAAARLESSKRFTKEICDEAGIPTAGW
CCEEEEECCCCCCEEHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ASFTDAGAARAYLDEHPAPIVIKADGLAAGKGVVVAATDEEAREAVAAMLEGGLHGASGA
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCH
EIVIEECLIGTEISLFAICDGEAAVFCGTASDHKRVGDGDTGPNTGGMGAIAPPPAASGA
HHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
VIGAAMERIVRPALRTMARRGTPFRGFLFAGLMLTASGPRLIEFNVRFGDPECETLLPLL
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHH
RTDLLPVLADAARGRLDPETRLDWRDGAAATVVMAAKGYPGAYERGHPIDLAGVAGLPET
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
TVFQAGTARRDGALVSAGGRVLAVTATGDDLREAIDRAYVAVRRIGFPGGFCRRDIGAKA
CEEECCCCCCCCCEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCC
V
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]