Click here to switch to the map view.

The map label for this gene is nuoE [H]

Identifier: 148260121

GI number: 148260121

Start: 1244908

End: 1245405

Strand: Reverse

Name: nuoE [H]

Synonym: Acry_1117

Alternate gene names: 148260121

Gene position: 1245405-1244908 (Counterclockwise)

Preceding gene: 148260122

Following gene: 148260120

Centisome position: 36.75

GC content: 70.68

Gene sequence:

>498_bases
ATGTCGACCGCTGAACCCGATCCGGCCGCCGCGCCGCTCGATGCCGCCCTGCGCGAGACGATCGCGACCTTTCTGCGCGA
GTCCGAAACCCCGCGCGCCGCCGGCATCGATGCGCTGAAGGCGGTGCAGAAGCGCCACGGCCATGTCTCGGACGACCATC
TCGCCGAAGTGGCCGCCCTGCTCGGCATGACACCCGCCGAGCTCGACGGTGTCGCCACCTTCTACAACCTGATCTTCCGC
CGCCCGGTGGGCCGCCACGTCATCCTGCTGTGCGACAGCGTGGCCTGCTGGGTGATGGGCGCCACCGCCGCGCGCAACCT
GCTGCGCCAGCGCCTCGGCATCGAGCCGGGGGAAACCACGCCGGACGGCCGCTTCACCCTCCTGCCGATCGTCTGCCTCG
GCTGCTGCGACCGCGCCCCGGCCATGATGATCGGCGACGACCTGCACGGCAACCTCACCCCCGAGCGGATCGACGCGATC
CTGGAGCAGTACAGGTGA

Upstream 100 bases:

>100_bases
CCGTCCTTTCCCCATCTGCAGATGATCCCGCTGATCAGCCGCGGCGCCTATGTCGCCGACCTGATCGCGATCATCGGCAG
CATCGATTTCGTGATGGCCG

Downstream 100 bases:

>100_bases
TGGCGATGGCGGACCGACCCCTCACTTCATACATCCAGCCCGGCCGCCAGCCGCTCGACATCGCCGGCTACGAGCGCGCC
GGCGGCTACGCGGCGATGCG

Product: NADH-quinone oxidoreductase subunit E

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E; NUO5 [H]

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSTAEPDPAAAPLDAALRETIATFLRESETPRAAGIDALKAVQKRHGHVSDDHLAEVAALLGMTPAELDGVATFYNLIFR
RPVGRHVILLCDSVACWVMGATAARNLLRQRLGIEPGETTPDGRFTLLPIVCLGCCDRAPAMMIGDDLHGNLTPERIDAI
LEQYR

Sequences:

>Translated_165_residues
MSTAEPDPAAAPLDAALRETIATFLRESETPRAAGIDALKAVQKRHGHVSDDHLAEVAALLGMTPAELDGVATFYNLIFR
RPVGRHVILLCDSVACWVMGATAARNLLRQRLGIEPGETTPDGRFTLLPIVCLGCCDRAPAMMIGDDLHGNLTPERIDAI
LEQYR
>Mature_164_residues
STAEPDPAAAPLDAALRETIATFLRESETPRAAGIDALKAVQKRHGHVSDDHLAEVAALLGMTPAELDGVATFYNLIFRR
PVGRHVILLCDSVACWVMGATAARNLLRQRLGIEPGETTPDGRFTLLPIVCLGCCDRAPAMMIGDDLHGNLTPERIDAIL
EQYR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=133, Percent_Identity=33.8345864661654, Blast_Score=94, Evalue=6e-20,
Organism=Escherichia coli, GI1788621, Length=164, Percent_Identity=50, Blast_Score=167, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17561328, Length=130, Percent_Identity=36.9230769230769, Blast_Score=96, Evalue=7e-21,
Organism=Drosophila melanogaster, GI18859877, Length=134, Percent_Identity=32.8358208955224, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21355353, Length=134, Percent_Identity=30.5970149253731, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17841; Mature: 17710

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01099 COMPLEX1_24K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAEPDPAAAPLDAALRETIATFLRESETPRAAGIDALKAVQKRHGHVSDDHLAEVAAL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LGMTPAELDGVATFYNLIFRRPVGRHVILLCDSVACWVMGATAARNLLRQRLGIEPGETT
HCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDGRFTLLPIVCLGCCDRAPAMMIGDDLHGNLTPERIDAILEQYR
CCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
STAEPDPAAAPLDAALRETIATFLRESETPRAAGIDALKAVQKRHGHVSDDHLAEVAAL
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LGMTPAELDGVATFYNLIFRRPVGRHVILLCDSVACWVMGATAARNLLRQRLGIEPGETT
HCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PDGRFTLLPIVCLGCCDRAPAMMIGDDLHGNLTPERIDAILEQYR
CCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]