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The map label for this gene is rbsB [H]

Identifier: 148260070

GI number: 148260070

Start: 1184972

End: 1185958

Strand: Reverse

Name: rbsB [H]

Synonym: Acry_1062

Alternate gene names: 148260070

Gene position: 1185958-1184972 (Counterclockwise)

Preceding gene: 148260071

Following gene: 148260069

Centisome position: 34.99

GC content: 63.32

Gene sequence:

>987_bases
ATGAAACTGAAGACACTGCTCGGCCCGGCCTGCCTCGGCGCCGTGCTTGCCGCATCGGCGGCGCTGCCGGCCCATGCCGC
GACCCAGCCGGCGATCCATTCGCAGAAGGGCAGCAACGGCAAGACGATCGCCATCTTCGTCCCCTCGCTCAGCAATCCGT
TCTTCGTGGCGGAGGAAAAATTCGCCGCCGAGGAAGCGACGAAGCTCGGCTACGGCACGATGGTCGCGAGCCATAACGGC
AATGCCTATACCCAGCTCAACCTGATCAAAACCGCGATCGCGCGCCACGTCGCCGCGATCATTCTCGACAATGCGGGCGC
GGATTCGTCGATCTCGGCGGTGAAGCTCGCGACCAAGGCGGGCGTTCCGGTCTTCCTGATCGACCGCGAGATCAACGCCA
AGGGCATCGCCAAGGCGCAGATCGTCTCCAACAACTTCCAGGGCGCGCAGCTGGAGGGGACGGAGTTCGCGAAGCTGATG
GGCTACAAGGGCACCTATGTCGAGCTGACCGGCCTGCCGTCGGACACCAACGCCAAGGTCCGCTCGCAGGGTGTCGCCAG
CGTGCTCAAGCATTTCTCCGGCATGAAGATGGTCGCCGTGCAGACCGCCGACTGGTCGCAGTCGAAGGGCTTCCAGGTGA
CGCAGACGCTGCTCGAGGCGCATCCGCACATCAAGGGCCTGATCGCCGACAACGACACGATGGCGATGGGTGCTGAAGCG
GCGCTGCTCGCGGCCCACAAGCCGAACGTGATCGTCACCGGCTTCGATGGCAGTCCGGACGTGATCAATTCGATTCGCAA
GGGCCAGATCAAGGCCGACGTGCTGCAGCCGATCGCGACCTTCTCGCGCGAGGCGGTGATCGAGGCGGACAAATACATCC
GCACCGGCAAGACCGGCAAGCCTGAGAAGCAGGAGCTGAACTGCACGCTGATCACCAAGGCGAATGTCGGCGACTACACC
AACTGGGCGATGAAATCGAAATCCTGA

Upstream 100 bases:

>100_bases
TGCCCTGGGGCCGGCGCCGCGGCAAGGAGGTAACGCCCTGAACAGCGTTCAGGAAGTTGCGAACAATCAACGAGTTCAAC
AGCAAGCGGAGGAACTGACC

Downstream 100 bases:

>100_bases
GCTGGCGGCTCGGCAACCACCCGGCGGCCCTTCGCGGGGCCGCCGGCAAAAATCGATCTGAACACCGACGGAACGGGGCG
GCGAATGCTCAACGGGGCGC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKLKTLLGPACLGAVLAASAALPAHAATQPAIHSQKGSNGKTIAIFVPSLSNPFFVAEEKFAAEEATKLGYGTMVASHNG
NAYTQLNLIKTAIARHVAAIILDNAGADSSISAVKLATKAGVPVFLIDREINAKGIAKAQIVSNNFQGAQLEGTEFAKLM
GYKGTYVELTGLPSDTNAKVRSQGVASVLKHFSGMKMVAVQTADWSQSKGFQVTQTLLEAHPHIKGLIADNDTMAMGAEA
ALLAAHKPNVIVTGFDGSPDVINSIRKGQIKADVLQPIATFSREAVIEADKYIRTGKTGKPEKQELNCTLITKANVGDYT
NWAMKSKS

Sequences:

>Translated_328_residues
MKLKTLLGPACLGAVLAASAALPAHAATQPAIHSQKGSNGKTIAIFVPSLSNPFFVAEEKFAAEEATKLGYGTMVASHNG
NAYTQLNLIKTAIARHVAAIILDNAGADSSISAVKLATKAGVPVFLIDREINAKGIAKAQIVSNNFQGAQLEGTEFAKLM
GYKGTYVELTGLPSDTNAKVRSQGVASVLKHFSGMKMVAVQTADWSQSKGFQVTQTLLEAHPHIKGLIADNDTMAMGAEA
ALLAAHKPNVIVTGFDGSPDVINSIRKGQIKADVLQPIATFSREAVIEADKYIRTGKTGKPEKQELNCTLITKANVGDYT
NWAMKSKS
>Mature_328_residues
MKLKTLLGPACLGAVLAASAALPAHAATQPAIHSQKGSNGKTIAIFVPSLSNPFFVAEEKFAAEEATKLGYGTMVASHNG
NAYTQLNLIKTAIARHVAAIILDNAGADSSISAVKLATKAGVPVFLIDREINAKGIAKAQIVSNNFQGAQLEGTEFAKLM
GYKGTYVELTGLPSDTNAKVRSQGVASVLKHFSGMKMVAVQTADWSQSKGFQVTQTLLEAHPHIKGLIADNDTMAMGAEA
ALLAAHKPNVIVTGFDGSPDVINSIRKGQIKADVLQPIATFSREAVIEADKYIRTGKTGKPEKQELNCTLITKANVGDYT
NWAMKSKS

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=297, Percent_Identity=32.6599326599327, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790526, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790674, Length=260, Percent_Identity=28.8461538461538, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789990, Length=249, Percent_Identity=28.1124497991968, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34574; Mature: 34574

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKTLLGPACLGAVLAASAALPAHAATQPAIHSQKGSNGKTIAIFVPSLSNPFFVAEEK
CCCHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECHH
FAAEEATKLGYGTMVASHNGNAYTQLNLIKTAIARHVAAIILDNAGADSSISAVKLATKA
HHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEHHHC
GVPVFLIDREINAKGIAKAQIVSNNFQGAQLEGTEFAKLMGYKGTYVELTGLPSDTNAKV
CCEEEEEECCCCCCCCEEEEEECCCCCCCEECCHHHHHHHCCCCCEEEEECCCCCCCCHH
RSQGVASVLKHFSGMKMVAVQTADWSQSKGFQVTQTLLEAHPHIKGLIADNDTMAMGAEA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCEECCCCE
ALLAAHKPNVIVTGFDGSPDVINSIRKGQIKADVLQPIATFSREAVIEADKYIRTGKTGK
EEEEECCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PEKQELNCTLITKANVGDYTNWAMKSKS
CCHHHCCEEEEEECCCCCCCCCEECCCC
>Mature Secondary Structure
MKLKTLLGPACLGAVLAASAALPAHAATQPAIHSQKGSNGKTIAIFVPSLSNPFFVAEEK
CCCHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECHH
FAAEEATKLGYGTMVASHNGNAYTQLNLIKTAIARHVAAIILDNAGADSSISAVKLATKA
HHHHHHHHCCCEEEEEECCCCEEEEHHHHHHHHHHHHEEEEEECCCCCCCCCEEEEHHHC
GVPVFLIDREINAKGIAKAQIVSNNFQGAQLEGTEFAKLMGYKGTYVELTGLPSDTNAKV
CCEEEEEECCCCCCCCEEEEEECCCCCCCEECCHHHHHHHCCCCCEEEEECCCCCCCCHH
RSQGVASVLKHFSGMKMVAVQTADWSQSKGFQVTQTLLEAHPHIKGLIADNDTMAMGAEA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCEECCCCE
ALLAAHKPNVIVTGFDGSPDVINSIRKGQIKADVLQPIATFSREAVIEADKYIRTGKTGK
EEEEECCCCEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PEKQELNCTLITKANVGDYTNWAMKSKS
CCHHHCCEEEEEECCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]