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The map label for this gene is ysgA [C]

Identifier: 148259911

GI number: 148259911

Start: 993980

End: 994645

Strand: Reverse

Name: ysgA [C]

Synonym: Acry_0900

Alternate gene names: 148259911

Gene position: 994645-993980 (Counterclockwise)

Preceding gene: 148259912

Following gene: 148259910

Centisome position: 29.35

GC content: 67.42

Gene sequence:

>666_bases
ATGGGTGAGATGATCAACCTGACCGCCTCCTGCGGGCACAAATTCCAGGCCTATGCCGCCGGCGACACCGCCTCGCAGCG
AGGCATCGTGATCGTGCAGGAAATCTTCGGCGTGAATTCGCACATGCGGCAGATCTCCGATCACCTGGCCTCGTTCGGCT
ACCGGGTCGTCACCCCGGCGCTGTTCGACCGGGTCGAGCGCGGCGTCGAACTCGGCTACGACGCCGCCGGGCGCGACAAG
GGCCTCGCCCTCCGCGCCGAGATCACCGACGAGGCCGTGATGCGCGACATCGAGGCCTCGGCCGCCGCCCTTTCGCCGCG
GCCCACGGGGATCATCGGCTATTGCTGGGGCGGCACCATCGCCTGGTGGGGGGCGACACGGAGCAAGAGCTTCAAGGCGG
CGATCGGCTGGTATGGCGGCGGCATCGCCGCCACAAGGTCGGAAAAGCCGAACTGCCCGGTGCAGCTGCATTTCGGCGCC
GAGGACAAGGGCATTCCGCTGGCGGATGTCGAGTCGATCCGCAAGGCGCAGCCGGATGTCGGAATTTTCGTCTACCCGGG
TGCCGAGCATGGCTTCGGCTGCCACGACCGGCCGAGCTTCAACCCCCGCGCCAACGAGATCGCCGAGCATCGCAGCCTCG
CCTTCTTCGCCGAACACGTCGTCTGA

Upstream 100 bases:

>100_bases
TTTCCTCCATCCCGGCGCTGGCGGTGCCTTTCAAGCGACCTATTTACGAGCGCCTCGCGCGGGATTTCGCGCGGTTCGCC
AAAACGGACGGAGCATGACG

Downstream 100 bases:

>100_bases
CGGGCGGCAGCCGTGAGCGCCGCGCGGCCCGGAGGGATTGAACCCGGCCGCGTGGCGTGCTGTTGAGGCGGCATGTCCGA
CGCACCCTCCGCAGACCGCA

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MGEMINLTASCGHKFQAYAAGDTASQRGIVIVQEIFGVNSHMRQISDHLASFGYRVVTPALFDRVERGVELGYDAAGRDK
GLALRAEITDEAVMRDIEASAAALSPRPTGIIGYCWGGTIAWWGATRSKSFKAAIGWYGGGIAATRSEKPNCPVQLHFGA
EDKGIPLADVESIRKAQPDVGIFVYPGAEHGFGCHDRPSFNPRANEIAEHRSLAFFAEHVV

Sequences:

>Translated_221_residues
MGEMINLTASCGHKFQAYAAGDTASQRGIVIVQEIFGVNSHMRQISDHLASFGYRVVTPALFDRVERGVELGYDAAGRDK
GLALRAEITDEAVMRDIEASAAALSPRPTGIIGYCWGGTIAWWGATRSKSFKAAIGWYGGGIAATRSEKPNCPVQLHFGA
EDKGIPLADVESIRKAQPDVGIFVYPGAEHGFGCHDRPSFNPRANEIAEHRSLAFFAEHVV
>Mature_220_residues
GEMINLTASCGHKFQAYAAGDTASQRGIVIVQEIFGVNSHMRQISDHLASFGYRVVTPALFDRVERGVELGYDAAGRDKG
LALRAEITDEAVMRDIEASAAALSPRPTGIIGYCWGGTIAWWGATRSKSFKAAIGWYGGGIAATRSEKPNCPVQLHFGAE
DKGIPLADVESIRKAQPDVGIFVYPGAEHGFGCHDRPSFNPRANEIAEHRSLAFFAEHVV

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=211, Percent_Identity=32.2274881516588, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 23849; Mature: 23718

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEMINLTASCGHKFQAYAAGDTASQRGIVIVQEIFGVNSHMRQISDHLASFGYRVVTPA
CCCEEEEHHHCCCCEEEEECCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCHHHHHH
LFDRVERGVELGYDAAGRDKGLALRAEITDEAVMRDIEASAAALSPRPTGIIGYCWGGTI
HHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
AWWGATRSKSFKAAIGWYGGGIAATRSEKPNCPVQLHFGAEDKGIPLADVESIRKAQPDV
EEECCCCCCCCEEEEEECCCCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCC
GIFVYPGAEHGFGCHDRPSFNPRANEIAEHRSLAFFAEHVV
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GEMINLTASCGHKFQAYAAGDTASQRGIVIVQEIFGVNSHMRQISDHLASFGYRVVTPA
CCEEEEHHHCCCCEEEEECCCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCCCHHHHHH
LFDRVERGVELGYDAAGRDKGLALRAEITDEAVMRDIEASAAALSPRPTGIIGYCWGGTI
HHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
AWWGATRSKSFKAAIGWYGGGIAATRSEKPNCPVQLHFGAEDKGIPLADVESIRKAQPDV
EEECCCCCCCCEEEEEECCCCEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCC
GIFVYPGAEHGFGCHDRPSFNPRANEIAEHRSLAFFAEHVV
EEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]