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The map label for this gene is purN [H]

Identifier: 148242451

GI number: 148242451

Start: 1198347

End: 1198979

Strand: Direct

Name: purN [H]

Synonym: SynRCC307_1352

Alternate gene names: 148242451

Gene position: 1198347-1198979 (Clockwise)

Preceding gene: 148242447

Following gene: 148242453

Centisome position: 53.86

GC content: 61.3

Gene sequence:

>633_bases
ATGGGACATGCCGCACAGCTCCTGTCACTTGACCCTAGCGATGCCCCACTGCGTTTGGCCGTTTTGGCCTCCGGTTCCGG
CAGCAACTTCCAGGCGCTGGTGGAAGCGCTGCGCAATGAGCCGCGTTTGCAGGTGGTGCTGCTGATCGTGAATCGCCCGG
GGTGTGGTGCGCAACAGCGGGCTGAACAACTGAACGTTCCCTGTCAGCTGATTGATCACACCCGCTTTGACAGCCGCGAA
GCAGTGGATGCTGCCGTGGTGCAAGCCCTGAAAAACGCGGCGGTGGAACTGGTGGTGATGGCTGGCTGGATGCGGATTGT
GACGCCGGCCTTGATCGGACCCTTTCAAGGCCGACTGCTCAATATTCATCCCAGCCTGCTGCCGTCGTTTCGCGGCATGC
ACGCCATCCGTCAAGCCCTGGCTGCTGGCGTCAGCCACACCGGATGCACCGTGCATGAAGTGGTGGAAGACGTGGACGCT
GGACCGGTTCTGGGGCAACAGGCCGTGGCCATCGAAGCCGGTGATGATGAGGCGAGCCTCTCAGCCCGCATCCACATCGC
TGAACATCAGCTATTGCCAGCTGTTGTGATCGAAAAGGGGCTCAGCCTGCTCAGCCATCGCACTCAGGGATAG

Upstream 100 bases:

>100_bases
GGGTAATCAACTCACCGCCTCGGTGGACCTGAAGTCCAAAGCTCTCGAGGCATTCAGCCAACCGCTCCACATCGTGCAAG
ACGGTGGGCAGGATGGAGAG

Downstream 100 bases:

>100_bases
AAGGGCAGCAGCGGCAACCCGGTTTGCTGAGGCAGGCCCGCCATCACATTGAGGCATTGCACAGCCTGACCGGCCTGTCC
CTTCAACAGGTTGTCGATCG

Product: folate-dependent phosphoribosylglycinamide formyltransferase PurN

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MGHAAQLLSLDPSDAPLRLAVLASGSGSNFQALVEALRNEPRLQVVLLIVNRPGCGAQQRAEQLNVPCQLIDHTRFDSRE
AVDAAVVQALKNAAVELVVMAGWMRIVTPALIGPFQGRLLNIHPSLLPSFRGMHAIRQALAAGVSHTGCTVHEVVEDVDA
GPVLGQQAVAIEAGDDEASLSARIHIAEHQLLPAVVIEKGLSLLSHRTQG

Sequences:

>Translated_210_residues
MGHAAQLLSLDPSDAPLRLAVLASGSGSNFQALVEALRNEPRLQVVLLIVNRPGCGAQQRAEQLNVPCQLIDHTRFDSRE
AVDAAVVQALKNAAVELVVMAGWMRIVTPALIGPFQGRLLNIHPSLLPSFRGMHAIRQALAAGVSHTGCTVHEVVEDVDA
GPVLGQQAVAIEAGDDEASLSARIHIAEHQLLPAVVIEKGLSLLSHRTQG
>Mature_209_residues
GHAAQLLSLDPSDAPLRLAVLASGSGSNFQALVEALRNEPRLQVVLLIVNRPGCGAQQRAEQLNVPCQLIDHTRFDSREA
VDAAVVQALKNAAVELVVMAGWMRIVTPALIGPFQGRLLNIHPSLLPSFRGMHAIRQALAAGVSHTGCTVHEVVEDVDAG
PVLGQQAVAIEAGDDEASLSARIHIAEHQLLPAVVIEKGLSLLSHRTQG

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=179, Percent_Identity=44.1340782122905, Blast_Score=161, Evalue=4e-40,
Organism=Homo sapiens, GI209869995, Length=179, Percent_Identity=44.1340782122905, Blast_Score=161, Evalue=4e-40,
Organism=Homo sapiens, GI209869993, Length=179, Percent_Identity=44.1340782122905, Blast_Score=161, Evalue=4e-40,
Organism=Escherichia coli, GI1788846, Length=181, Percent_Identity=39.7790055248619, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1787483, Length=111, Percent_Identity=31.5315315315315, Blast_Score=77, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=37.5661375661376, Blast_Score=140, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=31.1224489795918, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582400, Length=179, Percent_Identity=48.0446927374302, Blast_Score=163, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22244; Mature: 22113

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHAAQLLSLDPSDAPLRLAVLASGSGSNFQALVEALRNEPRLQVVLLIVNRPGCGAQQR
CCCHHCEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHH
AEQLNVPCQLIDHTRFDSREAVDAAVVQALKNAAVELVVMAGWMRIVTPALIGPFQGRLL
HHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
NIHPSLLPSFRGMHAIRQALAAGVSHTGCTVHEVVEDVDAGPVLGQQAVAIEAGDDEASL
EECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
SARIHIAEHQLLPAVVIEKGLSLLSHRTQG
EEEEEEHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GHAAQLLSLDPSDAPLRLAVLASGSGSNFQALVEALRNEPRLQVVLLIVNRPGCGAQQR
CCHHCEEECCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHH
AEQLNVPCQLIDHTRFDSREAVDAAVVQALKNAAVELVVMAGWMRIVTPALIGPFQGRLL
HHHCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
NIHPSLLPSFRGMHAIRQALAAGVSHTGCTVHEVVEDVDAGPVLGQQAVAIEAGDDEASL
EECHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCC
SARIHIAEHQLLPAVVIEKGLSLLSHRTQG
EEEEEEHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]