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The map label for this gene is ndhF [H]

Identifier: 148241926

GI number: 148241926

Start: 751703

End: 753562

Strand: Direct

Name: ndhF [H]

Synonym: SynRCC307_0827

Alternate gene names: 148241926

Gene position: 751703-753562 (Clockwise)

Preceding gene: 148241925

Following gene: 148241927

Centisome position: 33.79

GC content: 59.14

Gene sequence:

>1860_bases
TTGAACGCCCATCTCCCACTGTCCATCCAGCTCTCATGGCTGATTCCGTTGTACGGATTCAGCGGAGTTCTCCTGTCGCT
GCCCTGGGCGACCGGTTGGGTGAAGCGCAACGGCCCGCGCCCTGCCGCCTACTTGAACCTGTTGATGACTGTTCTGGGAG
TTGCCCACGGCAGCTTGATCTTGGCTTGGGTTCAGCAGAACGGTGGTGTGGTGATCGAGTGGCCCTGGTTTGAAACCTCT
GGCCTGGATTTGCGCATTGGCTTTGACCTGAGCCTCACCAACCTGGCGGCCCTGGAGTTCGTCACGATCATGAGCCTGCT
GGGGCAGGTGTTTGCCCTCGGCTATCTCGATAAGGAGTGGTCATTAGCCCGCTTCTATGCGCTGCTGGGCTTCTTCGAGG
GGGCGATGGCTGGCGTGGTGCTGAGCAGCAACTTGTTCTTGAGCTACTTCGTGCTCGAGATGCTCACCCTCTCCACCTAT
CTGCTGGTGGGCTTTTGGTACGCCCAGCCCCTGGTGGTCACCGCTGCCCGCGACGCGTTCCTCACCAAGCGCGTGGGTGA
TGTGCTGCTGTTGATGAGTGTGGTGGCTCTTGCTGCCTGGGCTGGATCCTTGGATTTCGAAGATCTCTATGCCTGGGCTG
CGCAAGTGAAAGCCGATGGCTCGGTGAGTCCCTTGCTTGGCACGTTGATCGGCTTGGGTTTGATTGCCGGCCCCATGGGC
AAATGTGCCCAGTTCCCCATGCACCTCTGGCTGGATGAAGCCATGGAGGGGCCCAATCCTGCGTCGATTTTGCGCAATTC
AGCGGTGGTCACCTGTGGAGCCTTGGTGCTGCTCAAGGTCATGCCGCTGCTGCAGCTCTCTCCAGTGGCCATTGATGTGT
TGCTGATCGTGGGCACCATCAGCGCCATTGGTGGCGCGCTGGTGGCGATTGCCCAAGTTGATATCAAGCGGGCGTTTTCC
TACGGCACCACCTCTTATCTGGGGTTGGTGTTCGTGGCCATTGCTGTGCAGCAACCGATCGTTGCGGTGCTGCTGCTGTT
TGCCCATGGCCTGGCCAAGGCGTTGATCTTCATGAGTGTGGGCAGCGTGATTGCCACCACCAACTGCCAAGACATCACCG
AGCTGGGTGGTTTGGGCCCACGCATGCCAGCCACCACAGGCAGCTACTTGATTGCGGGAGCTGGCCTCACCGGGGTTCTG
CCTTTGGGCTGTTTTTGGTGTTTTGGTTTGTTGGTGGATGGGCTTTGGCCCAATTACCCCTGGCTGGTGCCTGTTGTTCT
GCTCACCAACGCCCTCACGGCCTTCAACCTGGTGCGGGTGTTCCGCCAGGTATTCCTCGATAAGCCCCATCCCAAAACCA
AGCGCACACCTGAGGTGAATTGGCTGATGGCCCTGCCGATGGTGAGCCTGGCCGTGGTGGTGTTGCTGGTGCCCCTGATC
TTCCACCGCATCTACCCCATTGCTGGGGTCGAAGTGGTGACCATGGTCAGCATGGTGGCGGTGGTGGTCAGCAGCCTGGT
TGGGGTGGCATCGGCTGCGGCCATCTCACTGGATAAATTCGGCTCACGCTCTGTTTTCAAGCCCCTGCGAACCCTTCAGG
ATTTGCTGGCATACGACTTCTACACCGAGCGTTTTTATCGCTCCACCGTGGTGGCATTTGTGGCTTTTTTGGCCCGCATC
ACCGATGCCTTCGATCGCTTGCTGCTCAACCGCTTTGTGAATCGCATCGGCAGCAGTTCCATGGTGTCGGCTGAAGGTCT
GCGACTTGCTGTCAGTGGGCAGACCCAGTCCTATGTGCTGACGGTTCTGCTGGCCATCGTGCTGCTGCTTTCAGCGCTGG
TTTGGTGGAGGGCTGCCTGA

Upstream 100 bases:

>100_bases
CCGCACACGCCCAGGCTCTTGCCCAATGGTTGCGGGAGCTAAACGGTCCCGGCGTTAACTCGATACATTCTCCTGACTTG
CGACCCCCAGCCCGCGCCGC

Downstream 100 bases:

>100_bases
TGCTCACGGCTCTGTTATTGATCCCCTTCCTTGGGGGCCTGCTGCTGCTGCTTCTGCCCTCCAGCGTGGCTCCAGTTCGC
ATGCGCAGCGTGGCGATCGT

Product: NAD(P)H-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MNAHLPLSIQLSWLIPLYGFSGVLLSLPWATGWVKRNGPRPAAYLNLLMTVLGVAHGSLILAWVQQNGGVVIEWPWFETS
GLDLRIGFDLSLTNLAALEFVTIMSLLGQVFALGYLDKEWSLARFYALLGFFEGAMAGVVLSSNLFLSYFVLEMLTLSTY
LLVGFWYAQPLVVTAARDAFLTKRVGDVLLLMSVVALAAWAGSLDFEDLYAWAAQVKADGSVSPLLGTLIGLGLIAGPMG
KCAQFPMHLWLDEAMEGPNPASILRNSAVVTCGALVLLKVMPLLQLSPVAIDVLLIVGTISAIGGALVAIAQVDIKRAFS
YGTTSYLGLVFVAIAVQQPIVAVLLLFAHGLAKALIFMSVGSVIATTNCQDITELGGLGPRMPATTGSYLIAGAGLTGVL
PLGCFWCFGLLVDGLWPNYPWLVPVVLLTNALTAFNLVRVFRQVFLDKPHPKTKRTPEVNWLMALPMVSLAVVVLLVPLI
FHRIYPIAGVEVVTMVSMVAVVVSSLVGVASAAAISLDKFGSRSVFKPLRTLQDLLAYDFYTERFYRSTVVAFVAFLARI
TDAFDRLLLNRFVNRIGSSSMVSAEGLRLAVSGQTQSYVLTVLLAIVLLLSALVWWRAA

Sequences:

>Translated_619_residues
MNAHLPLSIQLSWLIPLYGFSGVLLSLPWATGWVKRNGPRPAAYLNLLMTVLGVAHGSLILAWVQQNGGVVIEWPWFETS
GLDLRIGFDLSLTNLAALEFVTIMSLLGQVFALGYLDKEWSLARFYALLGFFEGAMAGVVLSSNLFLSYFVLEMLTLSTY
LLVGFWYAQPLVVTAARDAFLTKRVGDVLLLMSVVALAAWAGSLDFEDLYAWAAQVKADGSVSPLLGTLIGLGLIAGPMG
KCAQFPMHLWLDEAMEGPNPASILRNSAVVTCGALVLLKVMPLLQLSPVAIDVLLIVGTISAIGGALVAIAQVDIKRAFS
YGTTSYLGLVFVAIAVQQPIVAVLLLFAHGLAKALIFMSVGSVIATTNCQDITELGGLGPRMPATTGSYLIAGAGLTGVL
PLGCFWCFGLLVDGLWPNYPWLVPVVLLTNALTAFNLVRVFRQVFLDKPHPKTKRTPEVNWLMALPMVSLAVVVLLVPLI
FHRIYPIAGVEVVTMVSMVAVVVSSLVGVASAAAISLDKFGSRSVFKPLRTLQDLLAYDFYTERFYRSTVVAFVAFLARI
TDAFDRLLLNRFVNRIGSSSMVSAEGLRLAVSGQTQSYVLTVLLAIVLLLSALVWWRAA
>Mature_619_residues
MNAHLPLSIQLSWLIPLYGFSGVLLSLPWATGWVKRNGPRPAAYLNLLMTVLGVAHGSLILAWVQQNGGVVIEWPWFETS
GLDLRIGFDLSLTNLAALEFVTIMSLLGQVFALGYLDKEWSLARFYALLGFFEGAMAGVVLSSNLFLSYFVLEMLTLSTY
LLVGFWYAQPLVVTAARDAFLTKRVGDVLLLMSVVALAAWAGSLDFEDLYAWAAQVKADGSVSPLLGTLIGLGLIAGPMG
KCAQFPMHLWLDEAMEGPNPASILRNSAVVTCGALVLLKVMPLLQLSPVAIDVLLIVGTISAIGGALVAIAQVDIKRAFS
YGTTSYLGLVFVAIAVQQPIVAVLLLFAHGLAKALIFMSVGSVIATTNCQDITELGGLGPRMPATTGSYLIAGAGLTGVL
PLGCFWCFGLLVDGLWPNYPWLVPVVLLTNALTAFNLVRVFRQVFLDKPHPKTKRTPEVNWLMALPMVSLAVVVLLVPLI
FHRIYPIAGVEVVTMVSMVAVVVSSLVGVASAAAISLDKFGSRSVFKPLRTLQDLLAYDFYTERFYRSTVVAFVAFLARI
TDAFDRLLLNRFVNRIGSSSMVSAEGLRLAVSGQTQSYVLTVLLAIVLLLSALVWWRAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=407, Percent_Identity=29.7297297297297, Blast_Score=157, Evalue=3e-38,
Organism=Escherichia coli, GI1788614, Length=333, Percent_Identity=36.036036036036, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1788831, Length=396, Percent_Identity=29.2929292929293, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1788827, Length=467, Percent_Identity=26.1241970021413, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788829, Length=403, Percent_Identity=26.0545905707196, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788613, Length=349, Percent_Identity=26.3610315186246, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI145693160, Length=462, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI2367154, Length=216, Percent_Identity=26.3888888888889, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 66805; Mature: 66805

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAHLPLSIQLSWLIPLYGFSGVLLSLPWATGWVKRNGPRPAAYLNLLMTVLGVAHGSLI
CCCCCCEEEEHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LAWVQQNGGVVIEWPWFETSGLDLRIGFDLSLTNLAALEFVTIMSLLGQVFALGYLDKEW
EEEEECCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SLARFYALLGFFEGAMAGVVLSSNLFLSYFVLEMLTLSTYLLVGFWYAQPLVVTAARDAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LTKRVGDVLLLMSVVALAAWAGSLDFEDLYAWAAQVKADGSVSPLLGTLIGLGLIAGPMG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
KCAQFPMHLWLDEAMEGPNPASILRNSAVVTCGALVLLKVMPLLQLSPVAIDVLLIVGTI
HHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SAIGGALVAIAQVDIKRAFSYGTTSYLGLVFVAIAVQQPIVAVLLLFAHGLAKALIFMSV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVIATTNCQDITELGGLGPRMPATTGSYLIAGAGLTGVLPLGCFWCFGLLVDGLWPNYP
HHHHEECCHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
WLVPVVLLTNALTAFNLVRVFRQVFLDKPHPKTKRTPEVNWLMALPMVSLAVVVLLVPLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FHRIYPIAGVEVVTMVSMVAVVVSSLVGVASAAAISLDKFGSRSVFKPLRTLQDLLAYDF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YTERFYRSTVVAFVAFLARITDAFDRLLLNRFVNRIGSSSMVSAEGLRLAVSGQTQSYVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHH
TVLLAIVLLLSALVWWRAA
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAHLPLSIQLSWLIPLYGFSGVLLSLPWATGWVKRNGPRPAAYLNLLMTVLGVAHGSLI
CCCCCCEEEEHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
LAWVQQNGGVVIEWPWFETSGLDLRIGFDLSLTNLAALEFVTIMSLLGQVFALGYLDKEW
EEEEECCCCEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
SLARFYALLGFFEGAMAGVVLSSNLFLSYFVLEMLTLSTYLLVGFWYAQPLVVTAARDAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
LTKRVGDVLLLMSVVALAAWAGSLDFEDLYAWAAQVKADGSVSPLLGTLIGLGLIAGPMG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
KCAQFPMHLWLDEAMEGPNPASILRNSAVVTCGALVLLKVMPLLQLSPVAIDVLLIVGTI
HHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SAIGGALVAIAQVDIKRAFSYGTTSYLGLVFVAIAVQQPIVAVLLLFAHGLAKALIFMSV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSVIATTNCQDITELGGLGPRMPATTGSYLIAGAGLTGVLPLGCFWCFGLLVDGLWPNYP
HHHHEECCHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
WLVPVVLLTNALTAFNLVRVFRQVFLDKPHPKTKRTPEVNWLMALPMVSLAVVVLLVPLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FHRIYPIAGVEVVTMVSMVAVVVSSLVGVASAAAISLDKFGSRSVFKPLRTLQDLLAYDF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YTERFYRSTVVAFVAFLARITDAFDRLLLNRFVNRIGSSSMVSAEGLRLAVSGQTQSYVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHH
TVLLAIVLLLSALVWWRAA
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]