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The map label for this gene is epsI

Identifier: 147675768

GI number: 147675768

Start: 2454872

End: 2455225

Strand: Reverse

Name: epsI

Synonym: VC0395_A2301

Alternate gene names: 147675768

Gene position: 2455225-2454872 (Counterclockwise)

Preceding gene: 147673549

Following gene: 147674823

Centisome position: 81.19

GC content: 52.26

Gene sequence:

>354_bases
ATGAAGAGTAAACGCGGTTTTACTTTGCTTGAAGTGCTGGTCGCGCTGGCAATTTTTGCTACCGCGGCGATCAGTGTGAT
CCGCTCGGTCAGTCAACACATCAATACGGTCAATTATCTTGAAGAGAAGATGTTTGCGGCCATGGTCGTGGATAACCAAA
TGGCGCAAGTGATGCTCAATCCGCAATCTTTAGCGGCGCGTGAGGGCAGTGAGCAGATGGCCGGACGGACTTGGTACTGG
AAGCTGAGTCCTGTCAAAACCGCCGACAATCTGCTCAAAGCCTTTGATGTCAGTGTCGCCACAGAAAAAGGCGCGACCCC
AGTCGTGACGGTGCGTAGCTATGTGGCGAACTAA

Upstream 100 bases:

>100_bases
ACGCTGAGCATTTTCCCTAAAGGGCAGGAGCCCGATGAGCAGTGGCGAGTGACCGCGCAAGAAAATGGCACTCTGCGTCT
ACTGGCTCCGGGAGAAAGTG

Downstream 100 bases:

>100_bases
CCAAGTATCTTCTCGCCAGAATATGGCGGGCTTTACTTTGATTGAAGTGTTGGTGGCGATTGCGATTTTCGCGAGCTTGA
GTGTGGGCGCCTATCAGGTG

Product: general secretion pathway protein I

Products: NA

Alternate protein names: Cholera toxin secretion protein epsI

Number of amino acids: Translated: 117; Mature: 117

Protein sequence:

>117_residues
MKSKRGFTLLEVLVALAIFATAAISVIRSVSQHINTVNYLEEKMFAAMVVDNQMAQVMLNPQSLAAREGSEQMAGRTWYW
KLSPVKTADNLLKAFDVSVATEKGATPVVTVRSYVAN

Sequences:

>Translated_117_residues
MKSKRGFTLLEVLVALAIFATAAISVIRSVSQHINTVNYLEEKMFAAMVVDNQMAQVMLNPQSLAAREGSEQMAGRTWYW
KLSPVKTADNLLKAFDVSVATEKGATPVVTVRSYVAN
>Mature_117_residues
MKSKRGFTLLEVLVALAIFATAAISVIRSVSQHINTVNYLEEKMFAAMVVDNQMAQVMLNPQSLAAREGSEQMAGRTWYW
KLSPVKTADNLLKAFDVSVATEKGATPVVTVRSYVAN

Specific function: Required for secretion of cholera toxin through the outer membrane

COG id: COG2165

COG function: function code NU; Type II secretory pathway, pseudopilin PulG

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP I family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPI_VIBCH (P45775)

Other databases:

- EMBL:   L33796
- EMBL:   AE003852
- PIR:   B82041
- RefSeq:   NP_232355.1
- ProteinModelPortal:   P45775
- SMR:   P45775
- GeneID:   2614891
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2728
- TIGR:   VC_2728
- HOGENOM:   HBG756901
- OMA:   NMPILEE
- ProtClustDB:   CLSK874899
- InterPro:   IPR010052
- InterPro:   IPR012902
- InterPro:   IPR001120
- InterPro:   IPR003413
- TIGRFAMs:   TIGR01707
- TIGRFAMs:   TIGR02532

Pfam domain/function: PF02501 GSPII_IJ; PF07963 N_methyl

EC number: NA

Molecular weight: Translated: 12837; Mature: 12837

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSKRGFTLLEVLVALAIFATAAISVIRSVSQHINTVNYLEEKMFAAMVVDNQMAQVMLN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQSLAAREGSEQMAGRTWYWKLSPVKTADNLLKAFDVSVATEKGATPVVTVRSYVAN
HHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKSKRGFTLLEVLVALAIFATAAISVIRSVSQHINTVNYLEEKMFAAMVVDNQMAQVMLN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PQSLAAREGSEQMAGRTWYWKLSPVKTADNLLKAFDVSVATEKGATPVVTVRSYVAN
HHHHHHCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301