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The map label for this gene is epsJ

Identifier: 147674823

GI number: 147674823

Start: 2454220

End: 2454885

Strand: Reverse

Name: epsJ

Synonym: VC0395_A2300

Alternate gene names: 147674823

Gene position: 2454885-2454220 (Counterclockwise)

Preceding gene: 147675768

Following gene: 147675385

Centisome position: 81.18

GC content: 52.25

Gene sequence:

>666_bases
ATGTGGCGAACTAACCAAGTATCTTCTCGCCAGAATATGGCGGGCTTTACTTTGATTGAAGTGTTGGTGGCGATTGCGAT
TTTCGCGAGCTTGAGTGTGGGCGCCTATCAGGTGCTCAATCAAGTCCAACGCAGCAATGAAATTTCTGCCGAGCGCACCG
CGCGTTTGGCTGAATTGCAACGCGCCATGGTGATCATGGATGCCGATTTTCGGCAGATGGCCCTGCGCCAATTTCGCACC
GATGGCGAAGCGCCGAGTGAGCAAATCCTACAATGGAAAGAATCGCTGCTCGATTCGGATCAGCACGGTTTGTTGTTTGT
ACGCTTGGGTTGGCATAACCCACAGCAACAATTTCCACGCGGTGAAGTGGCGAAAGTCGGTTACCGCCTGTTTGAAAACC
GCTTAGAGCGGGTCTGGTGGCGCTACCCAGATACTCCAGCGGGGCAGCAAGGGCTGATCTCTCCGTTGTTAACTGGGGTG
GAAGATTGGGCAGTACAGTTTTATTTGCAAGGTGAATGGAGTAAGGAGTGGGTGCCCACTAACGCCTTGCCTGAAGCCGT
GAAAGTGACCTTGCGCCTAAAAGATTACGGTGAGATTGAGCGGATATACCTTACAGGGGGCGGTTCACTCAATATGACGC
AAGAGAGTGTTGAAAATGCGGGCTAA

Upstream 100 bases:

>100_bases
AAGCTGAGTCCTGTCAAAACCGCCGACAATCTGCTCAAAGCCTTTGATGTCAGTGTCGCCACAGAAAAAGGCGCGACCCC
AGTCGTGACGGTGCGTAGCT

Downstream 100 bases:

>100_bases
ACAGCGCGGCGTGGCGTTAATTGTGATTCTGCTCCTGCTGGCGGTGATGGTCTCGATTGCTGCGACCATGGCCGAACGTC
TGTTTAGTCAGTTTCAGCGT

Product: general secretion pathway protein J

Products: NA

Alternate protein names: Cholera toxin secretion protein epsJ

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MWRTNQVSSRQNMAGFTLIEVLVAIAIFASLSVGAYQVLNQVQRSNEISAERTARLAELQRAMVIMDADFRQMALRQFRT
DGEAPSEQILQWKESLLDSDQHGLLFVRLGWHNPQQQFPRGEVAKVGYRLFENRLERVWWRYPDTPAGQQGLISPLLTGV
EDWAVQFYLQGEWSKEWVPTNALPEAVKVTLRLKDYGEIERIYLTGGGSLNMTQESVENAG

Sequences:

>Translated_221_residues
MWRTNQVSSRQNMAGFTLIEVLVAIAIFASLSVGAYQVLNQVQRSNEISAERTARLAELQRAMVIMDADFRQMALRQFRT
DGEAPSEQILQWKESLLDSDQHGLLFVRLGWHNPQQQFPRGEVAKVGYRLFENRLERVWWRYPDTPAGQQGLISPLLTGV
EDWAVQFYLQGEWSKEWVPTNALPEAVKVTLRLKDYGEIERIYLTGGGSLNMTQESVENAG
>Mature_221_residues
MWRTNQVSSRQNMAGFTLIEVLVAIAIFASLSVGAYQVLNQVQRSNEISAERTARLAELQRAMVIMDADFRQMALRQFRT
DGEAPSEQILQWKESLLDSDQHGLLFVRLGWHNPQQQFPRGEVAKVGYRLFENRLERVWWRYPDTPAGQQGLISPLLTGV
EDWAVQFYLQGEWSKEWVPTNALPEAVKVTLRLKDYGEIERIYLTGGGSLNMTQESVENAG

Specific function: Required for secretion of cholera toxin through the outer membrane

COG id: COG4795

COG function: function code U; Type II secretory pathway, component PulJ

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP J family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPJ_VIBCH (P45776)

Other databases:

- EMBL:   L33796
- EMBL:   AE003852
- PIR:   A82041
- RefSeq:   NP_232354.1
- ProteinModelPortal:   P45776
- SMR:   P45776
- GeneID:   2614890
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2727
- TIGR:   VC_2727
- HOGENOM:   HBG730817
- OMA:   QMVARTP
- ProtClustDB:   CLSK874898
- InterPro:   IPR010055
- InterPro:   IPR012902
- InterPro:   IPR001120
- TIGRFAMs:   TIGR01711
- TIGRFAMs:   TIGR02532

Pfam domain/function: PF07963 N_methyl

EC number: NA

Molecular weight: Translated: 25260; Mature: 25260

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00409 PROKAR_NTER_METHYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRTNQVSSRQNMAGFTLIEVLVAIAIFASLSVGAYQVLNQVQRSNEISAERTARLAELQ
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RAMVIMDADFRQMALRQFRTDGEAPSEQILQWKESLLDSDQHGLLFVRLGWHNPQQQFPR
HHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCC
GEVAKVGYRLFENRLERVWWRYPDTPAGQQGLISPLLTGVEDWAVQFYLQGEWSKEWVPT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHEEEEEEECCCCCCCCCC
NALPEAVKVTLRLKDYGEIERIYLTGGGSLNMTQESVENAG
CCCCHHHEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCC
>Mature Secondary Structure
MWRTNQVSSRQNMAGFTLIEVLVAIAIFASLSVGAYQVLNQVQRSNEISAERTARLAELQ
CCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RAMVIMDADFRQMALRQFRTDGEAPSEQILQWKESLLDSDQHGLLFVRLGWHNPQQQFPR
HHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCC
GEVAKVGYRLFENRLERVWWRYPDTPAGQQGLISPLLTGVEDWAVQFYLQGEWSKEWVPT
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHEEEEEEECCCCCCCCCC
NALPEAVKVTLRLKDYGEIERIYLTGGGSLNMTQESVENAG
CCCCHHHEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301