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The map label for this gene is sspA [H]

Identifier: 147675668

GI number: 147675668

Start: 97696

End: 98331

Strand: Direct

Name: sspA [H]

Synonym: VC0395_A0108

Alternate gene names: 147675668

Gene position: 97696-98331 (Clockwise)

Preceding gene: 147673543

Following gene: 147675024

Centisome position: 3.23

GC content: 43.55

Gene sequence:

>636_bases
ATGGCTGTAGCTGCCAACAAACGTTCTGTGATGACTCTATTTTCAAGTGCATCTGACATGTATAGCCATCAGGTTCGCAT
TGTGCTAGCTGAAAAAGGCGTCAGTTTTGAAGTTGAGTTGGTTGATGAGAACAACCTTCCAGCGGAACTGATTGAGCTAA
ATCCTTATAAAACAGTTCCAACACTGGTTGATCGCGAGCTTGCTCTGTATGACTCAAAGATCATCATGGAATACTTAGAT
GAGCGTTTCCCTCATCCGCCACTTATGCCTGTTTACCCAGTTGCACGTGGTAACAGCCGTCTGATGATTTATCGTATCGA
AAGAAACTGGTATTCATTGGCAGAAAAAGTAGTGAATGGTTCTCCTGAAGTTGCTGAAAATGCACGTAATAAGCTACGCA
ATGATCTGTTAACTTTAGGGCCTGTGTTTGCTGAGTTTGAATACTTCATGAGTGAAGAGTTCAGTCTGATCGATTGCTAT
CTTGCTCCACTGTTATGGCGTTTGCCTGTGCTGGGCATTGATTTAATTGGCCCTGGCTCAAAAGAGCTGAAAGTGTATAT
GAACCGTGTATTTGAACGTGATTCATTCTTGGCTTCACTGACTGAAGCTGAACGCGAGATGCGTCTGGCTCGCTAA

Upstream 100 bases:

>100_bases
ATTGTGCTATTATGCTGCGCTAATTCCCGATTGTTAACTCATCAGTGGAGGCTCTAGCCTCCATTGATTTTTTGTACCCG
CATCTGTACTGGAGGGCTCC

Downstream 100 bases:

>100_bases
TGGATGCCGGAATGGATATTGGTCACATGACACCACGCCGACCATATTTGCTACGTGCATTTTATGATTGGTTACTGGAA
AATGAATTAACGCCGCACCT

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAVAANKRSVMTLFSSASDMYSHQVRIVLAEKGVSFEVELVDENNLPAELIELNPYKTVPTLVDRELALYDSKIIMEYLD
ERFPHPPLMPVYPVARGNSRLMIYRIERNWYSLAEKVVNGSPEVAENARNKLRNDLLTLGPVFAEFEYFMSEEFSLIDCY
LAPLLWRLPVLGIDLIGPGSKELKVYMNRVFERDSFLASLTEAEREMRLAR

Sequences:

>Translated_211_residues
MAVAANKRSVMTLFSSASDMYSHQVRIVLAEKGVSFEVELVDENNLPAELIELNPYKTVPTLVDRELALYDSKIIMEYLD
ERFPHPPLMPVYPVARGNSRLMIYRIERNWYSLAEKVVNGSPEVAENARNKLRNDLLTLGPVFAEFEYFMSEEFSLIDCY
LAPLLWRLPVLGIDLIGPGSKELKVYMNRVFERDSFLASLTEAEREMRLAR
>Mature_210_residues
AVAANKRSVMTLFSSASDMYSHQVRIVLAEKGVSFEVELVDENNLPAELIELNPYKTVPTLVDRELALYDSKIIMEYLDE
RFPHPPLMPVYPVARGNSRLMIYRIERNWYSLAEKVVNGSPEVAENARNKLRNDLLTLGPVFAEFEYFMSEEFSLIDCYL
APLLWRLPVLGIDLIGPGSKELKVYMNRVFERDSFLASLTEAEREMRLAR

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789624, Length=211, Percent_Identity=72.0379146919431, Blast_Score=323, Evalue=4e-90,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 24271; Mature: 24140

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAANKRSVMTLFSSASDMYSHQVRIVLAEKGVSFEVELVDENNLPAELIELNPYKTVP
CCCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCCCH
TLVDRELALYDSKIIMEYLDERFPHPPLMPVYPVARGNSRLMIYRIERNWYSLAEKVVNG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHCC
SPEVAENARNKLRNDLLTLGPVFAEFEYFMSEEFSLIDCYLAPLLWRLPVLGIDLIGPGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEECCCC
KELKVYMNRVFERDSFLASLTEAEREMRLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVAANKRSVMTLFSSASDMYSHQVRIVLAEKGVSFEVELVDENNLPAELIELNPYKTVP
CCCCCCHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCCCH
TLVDRELALYDSKIIMEYLDERFPHPPLMPVYPVARGNSRLMIYRIERNWYSLAEKVVNG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCEEEEEEECCCHHHHHHHHHCC
SPEVAENARNKLRNDLLTLGPVFAEFEYFMSEEFSLIDCYLAPLLWRLPVLGIDLIGPGS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCEEEECCCC
KELKVYMNRVFERDSFLASLTEAEREMRLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]