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The map label for this gene is hisH

Identifier: 147675540

GI number: 147675540

Start: 730246

End: 730857

Strand: Direct

Name: hisH

Synonym: VC0395_A0706

Alternate gene names: 147675540

Gene position: 730246-730857 (Clockwise)

Preceding gene: 147673310

Following gene: 147674896

Centisome position: 24.15

GC content: 50.65

Gene sequence:

>612_bases
ATGACTCAGAATGTGGTGATTATTGATACTGGCTGCGCCAATATCTCCTCGGTCAAATTCGCCATTGAACGATTAGGTTA
TGCGGTGACGATTTCGCGCGATCCGCAAGTGGTACTCGCGGCAGATAAACTGTTTCTGCCCGGAGTTGGCACCGCGAGCG
AAGCGATGAAAAACCTCACCGAACGTGATTTGATCGAGTTGGTGAAACGGGTTGAGAAACCATTGCTTGGCATCTGCCTT
GGCATGCAATTGCTTGGCAAATTGTCGGAAGAAAAAGGCCAGAAAGCCGATGAGATTGTGCAGTGCTTAGGGTTAGTCGA
TGGCGAAGTCCGTTTGCTGCAAACCGGCGATCTGCCGTTGCCACACATGGGCTGGAACACGGTGCAAGTGAAAGAGGGGC
ATCCACTGTTTAACGGTATAGAACCTGATGCGTACTTCTACTTTGTGCACAGCTTTGCCATGCCCGTTGGCGATTACACC
ATTGCCCAGTGCGAATATGGTCAGCCGTTTAGCGCAGCGATTCAAGCAGGGAACTATTACGGGGTGCAATTTCACCCTGA
GCGCTCTTCAAAAGCGGGCGCACGCTTGATTCAAAACTTTCTTGAGCTTTAG

Upstream 100 bases:

>100_bases
ACCACATCATTGAGAGCCTGTTTAAAGCGTTTGGCCGCACGCTGCGTCAAGCCATCACGGTGCAAGGTAACGATCTGCCG
AGCAGTAAAGGGGTGCTCTG

Downstream 100 bases:

>100_bases
GGACAAGCGCTGGTCAAAGCTGGCGCAATACAAGGAACCACAATGATTATTCCCGCATTAGATTTGATTGAAGGTCAGGT
TGTGCGTCTGTATCAAGGTG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTQNVVIIDTGCANISSVKFAIERLGYAVTISRDPQVVLAADKLFLPGVGTASEAMKNLTERDLIELVKRVEKPLLGICL
GMQLLGKLSEEKGQKADEIVQCLGLVDGEVRLLQTGDLPLPHMGWNTVQVKEGHPLFNGIEPDAYFYFVHSFAMPVGDYT
IAQCEYGQPFSAAIQAGNYYGVQFHPERSSKAGARLIQNFLEL

Sequences:

>Translated_203_residues
MTQNVVIIDTGCANISSVKFAIERLGYAVTISRDPQVVLAADKLFLPGVGTASEAMKNLTERDLIELVKRVEKPLLGICL
GMQLLGKLSEEKGQKADEIVQCLGLVDGEVRLLQTGDLPLPHMGWNTVQVKEGHPLFNGIEPDAYFYFVHSFAMPVGDYT
IAQCEYGQPFSAAIQAGNYYGVQFHPERSSKAGARLIQNFLEL
>Mature_202_residues
TQNVVIIDTGCANISSVKFAIERLGYAVTISRDPQVVLAADKLFLPGVGTASEAMKNLTERDLIELVKRVEKPLLGICLG
MQLLGKLSEEKGQKADEIVQCLGLVDGEVRLLQTGDLPLPHMGWNTVQVKEGHPLFNGIEPDAYFYFVHSFAMPVGDYTI
AQCEYGQPFSAAIQAGNYYGVQFHPERSSKAGARLIQNFLEL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=200, Percent_Identity=61.5, Blast_Score=254, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=34.1121495327103, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_VIBCH (Q9KSX0)

Other databases:

- EMBL:   AE003852
- PIR:   C82238
- RefSeq:   NP_230781.1
- ProteinModelPortal:   Q9KSX0
- SMR:   Q9KSX0
- GeneID:   2614406
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1136
- TIGR:   VC_1136
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13170
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22274; Mature: 22143

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 185-185 ACT_SITE 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNVVIIDTGCANISSVKFAIERLGYAVTISRDPQVVLAADKLFLPGVGTASEAMKNLT
CCCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEEEECEEECCCCCCHHHHHHHCH
ERDLIELVKRVEKPLLGICLGMQLLGKLSEEKGQKADEIVQCLGLVDGEVRLLQTGDLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCC
PHMGWNTVQVKEGHPLFNGIEPDAYFYFVHSFAMPVGDYTIAQCEYGQPFSAAIQAGNYY
CCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCEE
GVQFHPERSSKAGARLIQNFLEL
EEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TQNVVIIDTGCANISSVKFAIERLGYAVTISRDPQVVLAADKLFLPGVGTASEAMKNLT
CCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCCCEEEEECEEECCCCCCHHHHHHHCH
ERDLIELVKRVEKPLLGICLGMQLLGKLSEEKGQKADEIVQCLGLVDGEVRLLQTGDLPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCC
PHMGWNTVQVKEGHPLFNGIEPDAYFYFVHSFAMPVGDYTIAQCEYGQPFSAAIQAGNYY
CCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCCEE
GVQFHPERSSKAGARLIQNFLEL
EEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301