Click here to switch to the map view.

The map label for this gene is hisA

Identifier: 147674896

GI number: 147674896

Start: 730900

End: 731637

Strand: Direct

Name: hisA

Synonym: VC0395_A0707

Alternate gene names: 147674896

Gene position: 730900-731637 (Clockwise)

Preceding gene: 147675540

Following gene: 147674704

Centisome position: 24.17

GC content: 50.14

Gene sequence:

>738_bases
ATGATTATTCCCGCATTAGATTTGATTGAAGGTCAGGTTGTGCGTCTGTATCAAGGTGATTACGGACAAGTGACCGAATA
TAAAGTAGACCCAGCGGAGCAGTTTAATTTGTACCACCAAGCTGGGGCAGGTTGGTTGCACTTGGTGGACTTAACTGGCG
CGAAAAATACCGCAGCACGCCAACTGAATTTGATTGCTCGCCTGCTCGCGAGCACACCCGCCAATATTCAAATTGGTGGT
GGAGTACGCAGTGAACAAGACGTGGTGGATCTGCTTGAAGCTGGCGCGCAGCGCGTGGTGGTGGGTTCAACCGCGGTTAA
ACAGCCGCAATTGGTCAAAAGCTGGATAGAAAAATACGGCGCTGAGAAAATTGTTTTAGCGTTGGACATCAACATTGATG
AACAAGGTACGCGCAAAGTCGCGATTTCTGGCTGGCAGGAAGATTCCGGCGTGACGATTGAAGCTTTGATTGATGATTAC
TTAACGGTTGGTTTACAGCACGTACTGTGCACCGATATTTCCCGCGATGGCACGTTGGCCGGTTCGAACGTTGAGCTGTA
TCGCGATCTCTGTCGTCAATATCCACAAGTGCAGTTCCAATCGTCCGGTGGCATTGGTTCACTTGCCGATATTGAGGCAT
TGAAAGGCACAGGCGTTGCGGGTGTGATTGTCGGTCGCGCGCTATTGGATGGCAAATTTACCGCGCAGGAGGCATTCGCA
TGTTGGCAAAACGGATAA

Upstream 100 bases:

>100_bases
CCCTGAGCGCTCTTCAAAAGCGGGCGCACGCTTGATTCAAAACTTTCTTGAGCTTTAGGGACAAGCGCTGGTCAAAGCTG
GCGCAATACAAGGAACCACA

Downstream 100 bases:

>100_bases
TTCCTTGTCTGGATGTACGTGATGGACAGGTAGTGAAAGGCGTTCAGTTTCGTAACCACGAAATCATTGGAGACATTGTG
CCACTGGCCAAACGTTACGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MIIPALDLIEGQVVRLYQGDYGQVTEYKVDPAEQFNLYHQAGAGWLHLVDLTGAKNTAARQLNLIARLLASTPANIQIGG
GVRSEQDVVDLLEAGAQRVVVGSTAVKQPQLVKSWIEKYGAEKIVLALDINIDEQGTRKVAISGWQEDSGVTIEALIDDY
LTVGLQHVLCTDISRDGTLAGSNVELYRDLCRQYPQVQFQSSGGIGSLADIEALKGTGVAGVIVGRALLDGKFTAQEAFA
CWQNG

Sequences:

>Translated_245_residues
MIIPALDLIEGQVVRLYQGDYGQVTEYKVDPAEQFNLYHQAGAGWLHLVDLTGAKNTAARQLNLIARLLASTPANIQIGG
GVRSEQDVVDLLEAGAQRVVVGSTAVKQPQLVKSWIEKYGAEKIVLALDINIDEQGTRKVAISGWQEDSGVTIEALIDDY
LTVGLQHVLCTDISRDGTLAGSNVELYRDLCRQYPQVQFQSSGGIGSLADIEALKGTGVAGVIVGRALLDGKFTAQEAFA
CWQNG
>Mature_245_residues
MIIPALDLIEGQVVRLYQGDYGQVTEYKVDPAEQFNLYHQAGAGWLHLVDLTGAKNTAARQLNLIARLLASTPANIQIGG
GVRSEQDVVDLLEAGAQRVVVGSTAVKQPQLVKSWIEKYGAEKIVLALDINIDEQGTRKVAISGWQEDSGVTIEALIDDY
LTVGLQHVLCTDISRDGTLAGSNVELYRDLCRQYPQVQFQSSGGIGSLADIEALKGTGVAGVIVGRALLDGKFTAQEAFA
CWQNG

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=244, Percent_Identity=65.5737704918033, Blast_Score=328, Evalue=2e-91,
Organism=Escherichia coli, GI1788336, Length=229, Percent_Identity=24.4541484716157, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_VIBCH (Q9KSW9)

Other databases:

- EMBL:   AE003852
- PIR:   D82238
- RefSeq:   NP_230782.1
- ProteinModelPortal:   Q9KSW9
- GeneID:   2614407
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1137
- TIGR:   VC_1137
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- ProtClustDB:   PRK00748
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 26339; Mature: 26339

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00626 RCC1_2

Important sites: ACT_SITE 7-7 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIPALDLIEGQVVRLYQGDYGQVTEYKVDPAEQFNLYHQAGAGWLHLVDLTGAKNTAAR
CCCCCCHHHCCCEEEEEECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
QLNLIARLLASTPANIQIGGGVRSEQDVVDLLEAGAQRVVVGSTAVKQPQLVKSWIEKYG
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCC
AEKIVLALDINIDEQGTRKVAISGWQEDSGVTIEALIDDYLTVGLQHVLCTDISRDGTLA
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GSNVELYRDLCRQYPQVQFQSSGGIGSLADIEALKGTGVAGVIVGRALLDGKFTAQEAFA
CCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHH
CWQNG
HHCCC
>Mature Secondary Structure
MIIPALDLIEGQVVRLYQGDYGQVTEYKVDPAEQFNLYHQAGAGWLHLVDLTGAKNTAAR
CCCCCCHHHCCCEEEEEECCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
QLNLIARLLASTPANIQIGGGVRSEQDVVDLLEAGAQRVVVGSTAVKQPQLVKSWIEKYG
HHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCC
AEKIVLALDINIDEQGTRKVAISGWQEDSGVTIEALIDDYLTVGLQHVLCTDISRDGTLA
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GSNVELYRDLCRQYPQVQFQSSGGIGSLADIEALKGTGVAGVIVGRALLDGKFTAQEAFA
CCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHH
CWQNG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301