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The map label for this gene is aroK

Identifier: 147675064

GI number: 147675064

Start: 2354335

End: 2354859

Strand: Reverse

Name: aroK

Synonym: VC0395_A2206

Alternate gene names: 147675064

Gene position: 2354859-2354335 (Counterclockwise)

Preceding gene: 147673064

Following gene: 147673423

Centisome position: 77.87

GC content: 47.24

Gene sequence:

>525_bases
ATGGCTGAGAAACGCAATATTTTCCTTGTTGGCCCTATGGGCGCTGGCAAAAGCACAATTGGTAGACACCTAGCTCAACA
ACTGCACATGGAGTTTATCGACTCTGATACGGTTATCGAAGAACGTACCGGAGCGGATATCGCATGGGTGTTTGATGTTG
AAGGTGAAGAAGGCTTCCGCAAGCGCGAAGAAGCCGTTATTAATGATCTAACCGAGCAACAGGGTATCGTACTGGCGACG
GGTGGTGGCTCAGTAATTAGTAAAGAGAACCGCAACCGACTGTCTGCTCGCGGTATCGTTGTATACCTCGAAACTACTAT
CGAAAAACAATTAGCACGTACCAATCGCGATAAAAAGCGCCCACTGCTGCAAACGGATTGCCCGCGTGAAGTGCTAGAGC
AATTGGCTGAAGATCGTAATCCTCTGTACGAAGAGATTGCGGACATCACGGTACGCACTGACGATCAAAGTGCAAAAGTG
GTAGCCAATCAGATCGTAAAAATGCTAGAAGAACACATAATGTAA

Upstream 100 bases:

>100_bases
TCGGTGAGGAATAGGTGCCACAAGCATTCCTATGGTTGGCGTATGTCCAGCAACTCTTGTGGCGATGTCATTGAATTAAC
GTTGTAAATTACTGCTGAAC

Downstream 100 bases:

>100_bases
TGTTTCTTCGAATTGGAGTGCAAAACCATGGAGCGGATTACGGTCAACTTAGGTGAACGTAGCTACCCAATCTCAATCGG
TGCCGGATTGTTTGCCAATC

Product: shikimate kinase I

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MAEKRNIFLVGPMGAGKSTIGRHLAQQLHMEFIDSDTVIEERTGADIAWVFDVEGEEGFRKREEAVINDLTEQQGIVLAT
GGGSVISKENRNRLSARGIVVYLETTIEKQLARTNRDKKRPLLQTDCPREVLEQLAEDRNPLYEEIADITVRTDDQSAKV
VANQIVKMLEEHIM

Sequences:

>Translated_174_residues
MAEKRNIFLVGPMGAGKSTIGRHLAQQLHMEFIDSDTVIEERTGADIAWVFDVEGEEGFRKREEAVINDLTEQQGIVLAT
GGGSVISKENRNRLSARGIVVYLETTIEKQLARTNRDKKRPLLQTDCPREVLEQLAEDRNPLYEEIADITVRTDDQSAKV
VANQIVKMLEEHIM
>Mature_173_residues
AEKRNIFLVGPMGAGKSTIGRHLAQQLHMEFIDSDTVIEERTGADIAWVFDVEGEEGFRKREEAVINDLTEQQGIVLATG
GGSVISKENRNRLSARGIVVYLETTIEKQLARTNRDKKRPLLQTDCPREVLEQLAEDRNPLYEEIADITVRTDDQSAKVV
ANQIVKMLEEHIM

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=173, Percent_Identity=81.5028901734104, Blast_Score=287, Evalue=3e-79,
Organism=Escherichia coli, GI1786587, Length=159, Percent_Identity=32.0754716981132, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_VIBC3 (A5F523)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F523
- SMR:   A5F523
- STRING:   A5F523
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2206
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK05057
- BioCyc:   VCHO345073:VC0395_A2206-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19619; Mature: 19487

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 36-36 BINDING 60-60 BINDING 82-82 BINDING 120-120 BINDING 139-139 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKRNIFLVGPMGAGKSTIGRHLAQQLHMEFIDSDTVIEERTGADIAWVFDVEGEEGFR
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCHHHH
KREEAVINDLTEQQGIVLATGGGSVISKENRNRLSARGIVVYLETTIEKQLARTNRDKKR
HHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCHHHCCEEEEEHHHHHHHHHHCCCHHCC
PLLQTDCPREVLEQLAEDRNPLYEEIADITVRTDDQSAKVVANQIVKMLEEHIM
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEKRNIFLVGPMGAGKSTIGRHLAQQLHMEFIDSDTVIEERTGADIAWVFDVEGEEGFR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCHHHH
KREEAVINDLTEQQGIVLATGGGSVISKENRNRLSARGIVVYLETTIEKQLARTNRDKKR
HHHHHHHHHHHHHCCEEEEECCCCEECCCCCCCHHHCCEEEEEHHHHHHHHHHCCCHHCC
PLLQTDCPREVLEQLAEDRNPLYEEIADITVRTDDQSAKVVANQIVKMLEEHIM
CCCCCCCHHHHHHHHHHCCCHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA