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The map label for this gene is pilQ [H]

Identifier: 147673064

GI number: 147673064

Start: 2355046

End: 2356782

Strand: Reverse

Name: pilQ [H]

Synonym: VC0395_A2207

Alternate gene names: 147673064

Gene position: 2356782-2355046 (Counterclockwise)

Preceding gene: 147673518

Following gene: 147675064

Centisome position: 77.93

GC content: 46.86

Gene sequence:

>1737_bases
TTGAAGTATAGAGATATAACGATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTGTGGGTTGGTTT
GGCACTTTGTGCTTCTTCCATGGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCA
ACAAAGAGAAGGCGGCGGTCTTAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGG
TTGAGCATTGAACTCCTGAAAACCGATGTGGCGGATGACAAGCTGTATCTGCTTGATGTGAAAGATTTTTCGACCCCTGT
TGAGAGCGTGGAAGTATTCCGTAAAGAGCCAAGCACACAACTGGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATA
CCTTAAAAGGAAAATACCTTGAGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAA
AAAGAAGGCAAGCTGATTTCAATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGG
TTTCAACTTAGTGGTTTCCGATTCGGTGGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGG
ACATTATTCTGCAAGTCAAAGGGCTCGATAAACGGGTCGATGGTAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGAC
TTGCGCGAGAAACAAGCGCTCGAAAAAGCCCGTTTAGCAGAAGAGTTGGGCGATTTAAAATCAGAAATCATCAAGATTAA
CTTTGCCAAAGCGTCTGACATTGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCA
GCATCGACGAACGAACTAACTCGCTATTGATCCGAGAGCTGCCCGATAATATTGCTGTGATCCGCGAGATCATTGAATCG
CTGGATATTCCGGTCAAACAAGTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGT
ACGCTGGGGCGTGATGTCGACCAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTAT
TGGCGGATGATGAATTTCCGGTGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCT
TTCCAAGTCGCTAAATTGGGCTCAGGTACGTTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAGGCCGAAAT
CATCTCTAGCCCTCGTTTGATTACCACTAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAAT
CGTCTTCCAGTGGTGCCTCTACGGTCGCGTTTAAAAAAGCGGTATTAAGCTTGAAGGTGACACCACAAATCACCCCAGAT
AACCGCTTGGTGCTCGACTTGAGTGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGTACGGGTGAAGCGGTATC
GATCGATACTCAGCGTATTGGCACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACACA
GCATCAATAATTCGGTAGATAAGGTGCCACTGCTGGGGGACTTGCCTGTTTTGGGGGCGTTATTCAGACGAACCTATGAG
CAAATGGGCAAAAGTGAACTGCTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Upstream 100 bases:

>100_bases
ACCCGAGTCGACGACAACTATTTGTTAATTAATGAAACTTTGCCTGATGGCTTAGGGTGTTGGAATCAGCGTAACGTCAA
GCTGGCTTTAAAATAATCGG

Downstream 100 bases:

>100_bases
CTTAGCGTGTGCGTAACAAGCGAACAAAAATTAAAGTTGCAATAGTGGCACCATATCTAGATAATTTCGGGTCTTATCAC
GAAATATCGGTGAGGAATAG

Product: fimbrial assembly protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKYRDITMKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEG
LSIELLKTDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLE
KEGKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELD
LREKQALEKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIES
LDIPVKQVQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIA
FQVAKLGSGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYE
QMGKSELLIFVTPKVVIQ

Sequences:

>Translated_578_residues
MKYRDITMKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEG
LSIELLKTDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLE
KEGKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELD
LREKQALEKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIES
LDIPVKQVQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIA
FQVAKLGSGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYE
QMGKSELLIFVTPKVVIQ
>Mature_578_residues
MKYRDITMKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEG
LSIELLKTDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLE
KEGKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELD
LREKQALEKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIES
LDIPVKQVQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIA
FQVAKLGSGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPD
NRLVLDLSVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYE
QMGKSELLIFVTPKVVIQ

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=413, Percent_Identity=37.5302663438257, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI87082242, Length=376, Percent_Identity=28.9893617021277, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 63249; Mature: 63249

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYRDITMKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAV
CCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCEEEECCCEEE
LIVELASPSAVVDVQKVQEGLSIELLKTDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQ
EEEEECCCCCEEEHHHHHCCCEEEEEEECCCCCEEEEEEEHHCCCCHHHHHHHHCCCCCE
LVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLISINFQDIPVRNVLQ
EEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEECCCCHHHHHH
LIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELD
HHHCCCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHHHCCCCEECCCCEEEEECCHHHC
LREKQALEKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTN
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCEEEEECCCCEEEECCCC
SLLIRELPDNIAVIREIIESLDIPVKQVQIEARIVTVKEGNLEELGVRWGVMSTNGSHSV
CEEEEECCCHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCCHHHHCEEEEEEECCCCCCC
GGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLGSGTLLDLELSALQ
CCCHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCCCHHHEEEEECCCCCEEEEEEHHHHC
NESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPD
CCCHHHHHCCCEEEECCCCCCCEECCCCCCEEECCCCCCHHHHEEEEEEEEEECCEECCC
NRLVLDLSVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVD
CEEEEEEEECCCCCCCEEECCCCCEEEEEHHHCCEEEEEECCCEEEEEHHHHHHCCCCCC
KVPLLGDLPVLGALFRRTYEQMGKSELLIFVTPKVVIQ
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEEEC
>Mature Secondary Structure
MKYRDITMKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAV
CCCCCEEHHCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCEEEECCCEEE
LIVELASPSAVVDVQKVQEGLSIELLKTDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQ
EEEEECCCCCEEEHHHHHCCCEEEEEEECCCCCEEEEEEEHHCCCCHHHHHHHHCCCCCE
LVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLISINFQDIPVRNVLQ
EEEEECCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEEECCCCHHHHHH
LIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELD
HHHCCCCCEEEEECCEEEEEEEEECCCCHHHHHHHHHHHCCCCEECCCCEEEEECCHHHC
LREKQALEKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTN
HHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEECCCCCEEEEECCCCEEEECCCC
SLLIRELPDNIAVIREIIESLDIPVKQVQIEARIVTVKEGNLEELGVRWGVMSTNGSHSV
CEEEEECCCHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCCHHHHCEEEEEEECCCCCCC
GGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLGSGTLLDLELSALQ
CCCHHHHHHHCCCCCCCCCCHHHHHEEEEECCCCCCHHHEEEEECCCCCEEEEEEHHHHC
NESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPD
CCCHHHHHCCCEEEECCCCCCCEECCCCCCEEECCCCCCHHHHEEEEEEEEEECCEECCC
NRLVLDLSVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVD
CEEEEEEEECCCCCCCEEECCCCCEEEEEHHHCCEEEEEECCCEEEEEHHHHHHCCCCCC
KVPLLGDLPVLGALFRRTYEQMGKSELLIFVTPKVVIQ
CCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]