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The map label for this gene is kefG [H]

Identifier: 147674366

GI number: 147674366

Start: 2330483

End: 2331073

Strand: Reverse

Name: kefG [H]

Synonym: VC0395_A2185

Alternate gene names: 147674366

Gene position: 2331073-2330483 (Counterclockwise)

Preceding gene: 147673169

Following gene: 147673616

Centisome position: 77.08

GC content: 50.93

Gene sequence:

>591_bases
GTGCGCAACTTGGTCAACCGAGAGCAAACGAAAGCCAAAGTGCTGGTGGTGTATGCGCACCCAGATCCACAACACTCCGT
TGCCAATCAGGTGATGATGGAAAAGATTGCCGATTTAGAGCATGTTTCGATTATCGACCTCTACGCCACTTACCCCAATT
TCTTTATTGATGTGCATGCCGAGCAGCAGCGCTTGCGGGAACATGATGTGCTGGTGTTGCAACATCCCCTCTATATGTAT
TCCTGCCCTGCTCTTTTAAAAGAGTGGATTGATCGCGTGTTGAGCAAAGGTTTCGCCTTCGGTAAACAGAGTGAGCTGAA
AGGGAAACTTTGGCGCAGTGTGATTACCACTGGCGGCAACCACACCGCCTACGATCAACGCGGTTATAACCGTTATCCGC
TGAATGAAATCCTGCAGCCGTTCGAGCTCAGTGCGGCTTTGTGTCAGATGCACTGGATTGAGCCTTTGGTGCTGTATTGG
TCACGCAACATCAGTGATCAAGAGCGTGCGCAACATGCAGAGCGTTATCGGCAATGGTTACAAGAGATCGCCCACTGGCA
AGGAGAGCCGTATGACGCCACTGACCAGTGA

Upstream 100 bases:

>100_bases
GAATTTTCGCTCGGAATAGTAGGTGGAATAAGGATAAATGTCGATCATTATACAAATTGGGTTATGATGCTGAGTAACGG
ATTAGTAACAAATCAGCGGA

Downstream 100 bases:

>100_bases
TTTTCTCAGTAGCAGTGTCGTCTTTCTTAGCGCTGCGGTGATTGCCGTCCCCCTTGCGCAGCGCGCGGGATTAGGCTCTG
TGCTTGGCTATTTGCTGGCG

Product: glutathione-regulated potassium-efflux system ancillary protein KefG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MRNLVNREQTKAKVLVVYAHPDPQHSVANQVMMEKIADLEHVSIIDLYATYPNFFIDVHAEQQRLREHDVLVLQHPLYMY
SCPALLKEWIDRVLSKGFAFGKQSELKGKLWRSVITTGGNHTAYDQRGYNRYPLNEILQPFELSAALCQMHWIEPLVLYW
SRNISDQERAQHAERYRQWLQEIAHWQGEPYDATDQ

Sequences:

>Translated_196_residues
MRNLVNREQTKAKVLVVYAHPDPQHSVANQVMMEKIADLEHVSIIDLYATYPNFFIDVHAEQQRLREHDVLVLQHPLYMY
SCPALLKEWIDRVLSKGFAFGKQSELKGKLWRSVITTGGNHTAYDQRGYNRYPLNEILQPFELSAALCQMHWIEPLVLYW
SRNISDQERAQHAERYRQWLQEIAHWQGEPYDATDQ
>Mature_196_residues
MRNLVNREQTKAKVLVVYAHPDPQHSVANQVMMEKIADLEHVSIIDLYATYPNFFIDVHAEQQRLREHDVLVLQHPLYMY
SCPALLKEWIDRVLSKGFAFGKQSELKGKLWRSVITTGGNHTAYDQRGYNRYPLNEILQPFELSAALCQMHWIEPLVLYW
SRNISDQERAQHAERYRQWLQEIAHWQGEPYDATDQ

Specific function: Required for full activity of kefB [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefG subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=226, Percent_Identity=30.9734513274336, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789750, Length=170, Percent_Identity=57.6470588235294, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1786231, Length=171, Percent_Identity=39.1812865497076, Blast_Score=122, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.- [C]

Molecular weight: Translated: 23135; Mature: 23135

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLVNREQTKAKVLVVYAHPDPQHSVANQVMMEKIADLEHVSIIDLYATYPNFFIDVHA
CCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECH
EQQRLREHDVLVLQHPLYMYSCPALLKEWIDRVLSKGFAFGKQSELKGKLWRSVITTGGN
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
HTAYDQRGYNRYPLNEILQPFELSAALCQMHWIEPLVLYWSRNISDQERAQHAERYRQWL
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QEIAHWQGEPYDATDQ
HHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRNLVNREQTKAKVLVVYAHPDPQHSVANQVMMEKIADLEHVSIIDLYATYPNFFIDVHA
CCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEEECH
EQQRLREHDVLVLQHPLYMYSCPALLKEWIDRVLSKGFAFGKQSELKGKLWRSVITTGGN
HHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
HTAYDQRGYNRYPLNEILQPFELSAALCQMHWIEPLVLYWSRNISDQERAQHAERYRQWL
CCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QEIAHWQGEPYDATDQ
HHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA