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The map label for this gene is kefB [H]

Identifier: 147673616

GI number: 147673616

Start: 2328700

End: 2330502

Strand: Reverse

Name: kefB [H]

Synonym: VC0395_A2184

Alternate gene names: 147673616

Gene position: 2330502-2328700 (Counterclockwise)

Preceding gene: 147674366

Following gene: 147675564

Centisome position: 77.07

GC content: 51.64

Gene sequence:

>1803_bases
ATGACGCCACTGACCAGTGATTTTCTCAGTAGCAGTGTCGTCTTTCTTAGCGCTGCGGTGATTGCCGTCCCCCTTGCGCA
GCGCGCGGGATTAGGCTCTGTGCTTGGCTATTTGCTGGCGGGGATTGCGATTGGTCCATGGGGACTGGGCTTGATCCGCG
ATGTGCAAGCCATCATGCATTTTGCTGAGTTTGGCGTGGTGCTACTGCTGTTTCTGATTGGCTTAGAGCTCAACCCGAAA
AAACTGTGGAATCTACGCGGCCCCATTTTAGGGCTAGGCGGCGCGCAAGTGGTGGTGACCACTGCGGTGCTTTCCAGTGT
CGCGTATTTACTGGGTGTCAGTTGGCAAAGTGCGTTAGTCATCGGTATGGCGCTGGCGCTTTCATCGACCGCGATCGCTC
TGCGCGTGATTGAAGAGCACGGCTTAACGCGTACTGAGGCGGGACAATCGGGATTTGCGGTGCTGCTGTTTCAAGATATT
GCGGTGATCCCCATGTTGGCACTGCTGCCAATCCTCGCTGGAAACAGCAGTGGCGATTGGCTGAATGCCCTGTGGATGAC
GGGAGGAATTGCTGCTTTGTTACTGGGCGGACATTTTCTGCTTAACCCGCTGTTTCGATACATCGCATTAAGTGGCGTGC
GCGAACTGTTTACTGTCGCGGCTTTGCTCTTGGTGGTTGGCATCGCGTTGCTGATGCAGAAGGTTGGTCTTTCGATGGCG
CTAGGTACTTTTTTAGCTGGGGTGATCCTCGCAGAAAGCGAGTTTCGTCATGAGCTAGAAATCGCTATCGATCCTTTTAA
AGGCTTATTGCTTGGTCTGTTTTTTATTTCGGTGGGTATGGCAGTGGACGTGGGTTTGTTACTCGTCAAGCCTCTGCAGA
TTATGCTGGCCGTGTTGGGCTTAGTGATTGTCAAAAGTCTAGTGCTCTATCTGCTGGCACGCCTGTCGGGTACGGTTGCC
AAAGCGCGCAGCAAAATGGCGGCGATTTTAAGCCAAGGGGGGGAATTCGCCTTCGTGATTTTTACCGCCGCCAGTGCGGA
GGGCTTGCTGACGGCTTCGCAAGTCTCCTTCCTTCTGGTGGTGGTGAGCCTTTCTATGGTGACCACGCCGTTATTACTCT
CTGCCCAAAAATACTGGTTTGCCCGCCAGCTCAATATTGAAGAAAACCCACTGACGCCGGATGTCGAAAATAAAGAGCCG
CGAGTGATCATTGTCGGTTTTGGCCGCTTTGGTCAGATAGTGGGGCGTCTGCTGTACGCCAACAAAATCAAAGTCACTAT
TTTGGAAAGTGACGCCAGCCAAGTACGTTTACTGCGTAAATACGGCTACAAAGTCTTTTATGGGGATGCCACCAATCTGG
AACTATTACGCGCTGCGGGTGTTGAGCAAGCAGAGGCCTTGGTGGTTTGTACTGACGACCCTGAGCAAGTGATCACCATA
GTAGAGCTGTGTCAGCAGCATTTTCCGAATCTGAAATTGTTGGCGCGAGCACGCAGTCGGGTCGAAGCTTATCAACTGAT
GAGTCTCGGAGTACAAAATTACACCCGAGAAACCTTCTTGAGCGCACTCGACTTGGGGCGCAAAGCCTTGGTTCAACTTG
GCATGCATCCTTATCAAGCGAAACGTGCCGAAGAGCATTTTCATCGTCTCGATAAAACTATGCTCAAAGAATTGCTACCG
CAACATAATGAAGATAAACAACTCGAACTGCGCGCCAAAGAAGCGCGTACCGAACTTGAAGAAATATTTAGCCGTGAGAT
GGACAGTGATCAGCTCTCACGCCGATATTGGGAAAAGGAGTAG

Upstream 100 bases:

>100_bases
CTGTATTGGTCACGCAACATCAGTGATCAAGAGCGTGCGCAACATGCAGAGCGTTATCGGCAATGGTTACAAGAGATCGC
CCACTGGCAAGGAGAGCCGT

Downstream 100 bases:

>100_bases
TACAATCTCGTGAAACAGAAAAAACGTTTTATCGCTGGCGCAAACTGCCCACAGTGCAAGACCGCCGATACGTTACAATG
GTGGGTAGAGAACAGCATTG

Product: glutathione-regulated potassium-efflux system protein KefB

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MTPLTSDFLSSSVVFLSAAVIAVPLAQRAGLGSVLGYLLAGIAIGPWGLGLIRDVQAIMHFAEFGVVLLLFLIGLELNPK
KLWNLRGPILGLGGAQVVVTTAVLSSVAYLLGVSWQSALVIGMALALSSTAIALRVIEEHGLTRTEAGQSGFAVLLFQDI
AVIPMLALLPILAGNSSGDWLNALWMTGGIAALLLGGHFLLNPLFRYIALSGVRELFTVAALLLVVGIALLMQKVGLSMA
LGTFLAGVILAESEFRHELEIAIDPFKGLLLGLFFISVGMAVDVGLLLVKPLQIMLAVLGLVIVKSLVLYLLARLSGTVA
KARSKMAAILSQGGEFAFVIFTAASAEGLLTASQVSFLLVVVSLSMVTTPLLLSAQKYWFARQLNIEENPLTPDVENKEP
RVIIVGFGRFGQIVGRLLYANKIKVTILESDASQVRLLRKYGYKVFYGDATNLELLRAAGVEQAEALVVCTDDPEQVITI
VELCQQHFPNLKLLARARSRVEAYQLMSLGVQNYTRETFLSALDLGRKALVQLGMHPYQAKRAEEHFHRLDKTMLKELLP
QHNEDKQLELRAKEARTELEEIFSREMDSDQLSRRYWEKE

Sequences:

>Translated_600_residues
MTPLTSDFLSSSVVFLSAAVIAVPLAQRAGLGSVLGYLLAGIAIGPWGLGLIRDVQAIMHFAEFGVVLLLFLIGLELNPK
KLWNLRGPILGLGGAQVVVTTAVLSSVAYLLGVSWQSALVIGMALALSSTAIALRVIEEHGLTRTEAGQSGFAVLLFQDI
AVIPMLALLPILAGNSSGDWLNALWMTGGIAALLLGGHFLLNPLFRYIALSGVRELFTVAALLLVVGIALLMQKVGLSMA
LGTFLAGVILAESEFRHELEIAIDPFKGLLLGLFFISVGMAVDVGLLLVKPLQIMLAVLGLVIVKSLVLYLLARLSGTVA
KARSKMAAILSQGGEFAFVIFTAASAEGLLTASQVSFLLVVVSLSMVTTPLLLSAQKYWFARQLNIEENPLTPDVENKEP
RVIIVGFGRFGQIVGRLLYANKIKVTILESDASQVRLLRKYGYKVFYGDATNLELLRAAGVEQAEALVVCTDDPEQVITI
VELCQQHFPNLKLLARARSRVEAYQLMSLGVQNYTRETFLSALDLGRKALVQLGMHPYQAKRAEEHFHRLDKTMLKELLP
QHNEDKQLELRAKEARTELEEIFSREMDSDQLSRRYWEKE
>Mature_599_residues
TPLTSDFLSSSVVFLSAAVIAVPLAQRAGLGSVLGYLLAGIAIGPWGLGLIRDVQAIMHFAEFGVVLLLFLIGLELNPKK
LWNLRGPILGLGGAQVVVTTAVLSSVAYLLGVSWQSALVIGMALALSSTAIALRVIEEHGLTRTEAGQSGFAVLLFQDIA
VIPMLALLPILAGNSSGDWLNALWMTGGIAALLLGGHFLLNPLFRYIALSGVRELFTVAALLLVVGIALLMQKVGLSMAL
GTFLAGVILAESEFRHELEIAIDPFKGLLLGLFFISVGMAVDVGLLLVKPLQIMLAVLGLVIVKSLVLYLLARLSGTVAK
ARSKMAAILSQGGEFAFVIFTAASAEGLLTASQVSFLLVVVSLSMVTTPLLLSAQKYWFARQLNIEENPLTPDVENKEPR
VIIVGFGRFGQIVGRLLYANKIKVTILESDASQVRLLRKYGYKVFYGDATNLELLRAAGVEQAEALVVCTDDPEQVITIV
ELCQQHFPNLKLLARARSRVEAYQLMSLGVQNYTRETFLSALDLGRKALVQLGMHPYQAKRAEEHFHRLDKTMLKELLPQ
HNEDKQLELRAKEARTELEEIFSREMDSDQLSRRYWEKE

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=358, Percent_Identity=26.8156424581006, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI1789749, Length=597, Percent_Identity=58.2914572864322, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1786232, Length=601, Percent_Identity=45.5906821963394, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1786685, Length=534, Percent_Identity=30.3370786516854, Blast_Score=191, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 65386; Mature: 65255

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLTSDFLSSSVVFLSAAVIAVPLAQRAGLGSVLGYLLAGIAIGPWGLGLIRDVQAIMH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FAEFGVVLLLFLIGLELNPKKLWNLRGPILGLGGAQVVVTTAVLSSVAYLLGVSWQSALV
HHHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHH
IGMALALSSTAIALRVIEEHGLTRTEAGQSGFAVLLFQDIAVIPMLALLPILAGNSSGDW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCH
LNALWMTGGIAALLLGGHFLLNPLFRYIALSGVRELFTVAALLLVVGIALLMQKVGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFLAGVILAESEFRHELEIAIDPFKGLLLGLFFISVGMAVDVGLLLVKPLQIMLAVLG
HHHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIVKSLVLYLLARLSGTVAKARSKMAAILSQGGEFAFVIFTAASAEGLLTASQVSFLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHH
VVSLSMVTTPLLLSAQKYWFARQLNIEENPLTPDVENKEPRVIIVGFGRFGQIVGRLLYA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
NKIKVTILESDASQVRLLRKYGYKVFYGDATNLELLRAAGVEQAEALVVCTDDPEQVITI
CCEEEEEECCCHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHH
VELCQQHFPNLKLLARARSRVEAYQLMSLGVQNYTRETFLSALDLGRKALVQLGMHPYQA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KRAEEHFHRLDKTMLKELLPQHNEDKQLELRAKEARTELEEIFSREMDSDQLSRRYWEKE
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TPLTSDFLSSSVVFLSAAVIAVPLAQRAGLGSVLGYLLAGIAIGPWGLGLIRDVQAIMH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
FAEFGVVLLLFLIGLELNPKKLWNLRGPILGLGGAQVVVTTAVLSSVAYLLGVSWQSALV
HHHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHH
IGMALALSSTAIALRVIEEHGLTRTEAGQSGFAVLLFQDIAVIPMLALLPILAGNSSGDW
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCH
LNALWMTGGIAALLLGGHFLLNPLFRYIALSGVRELFTVAALLLVVGIALLMQKVGLSMA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTFLAGVILAESEFRHELEIAIDPFKGLLLGLFFISVGMAVDVGLLLVKPLQIMLAVLG
HHHHHHHHHHHHHHHCCHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVIVKSLVLYLLARLSGTVAKARSKMAAILSQGGEFAFVIFTAASAEGLLTASQVSFLLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHH
VVSLSMVTTPLLLSAQKYWFARQLNIEENPLTPDVENKEPRVIIVGFGRFGQIVGRLLYA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHH
NKIKVTILESDASQVRLLRKYGYKVFYGDATNLELLRAAGVEQAEALVVCTDDPEQVITI
CCEEEEEECCCHHHHHHHHHCCCEEEECCCCCHHHHHHCCCCCCCEEEEECCCHHHHHHH
VELCQQHFPNLKLLARARSRVEAYQLMSLGVQNYTRETFLSALDLGRKALVQLGMHPYQA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
KRAEEHFHRLDKTMLKELLPQHNEDKQLELRAKEARTELEEIFSREMDSDQLSRRYWEKE
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA