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The map label for this gene is dapA

Identifier: 147673601

GI number: 147673601

Start: 1859550

End: 1860428

Strand: Reverse

Name: dapA

Synonym: VC0395_A1738

Alternate gene names: 147673601

Gene position: 1860428-1859550 (Counterclockwise)

Preceding gene: 147673902

Following gene: 147674077

Centisome position: 61.52

GC content: 48.92

Gene sequence:

>879_bases
ATGTTTTCAGGAAGTATCGTTGCGTTAATTACCCCATTTACTCCGGACGGTGAAGTCGATTATATCAGCCTGAAAAAGCT
GGTCGATTTCCATGTTGATGCAGGAACCGATGCGATTGTTTCCGTTGGCACCACCGGTGAATCCGCGACGCTAACGGTCG
AAGAACACGTGAAAGTAGTAGCAAAAACGGTGGAGTTCGCTGAAGGTCGTCTGCCTATTATTGCTGGAACAGGCGCAAAT
GCGACCCATGAAGCGGTCACTTTTAGCCGCCTGCTGAACAACACAGGTATTGCAGGGTATTTGAGTGTCACGCCTTATTA
CAATAAGCCGACCCAAGAAGGTCTGTTCCTGCACTACAACGCGATTGCGCAAGAAACCGATATTCCCGTGATTTTGTACA
ACGTGCCGGGACGTACCGCTGTGGATATGCGTCCAGAAACCGTTGCCCGTTTATCTGAAATCAAGAACATTGTCGCACTG
AAAGATGCAACCGGTGATTTAAGCCGTGTTGCGAAGCACCGTGAGATGTGTAAAGAAGGTTTTGTCCTACTCAGCGGTGA
TGATGCCACAGGCCTTGAGTTCGTCAAACTCGGGGGGCAAGGTGTGATTTCAGTGACCAACAATATCGCTGCTGCCGACA
TGGCGAAGATGATGCATTTGGCGCTGGATGGTAAGTTTGACGAAGCGGCGAGCATCAACCAACGCTTGATGACACTGCAT
AAAAATCTGTTTATCGAATCGAGCCCAATTCCAGTGAAATGGGCAGCCCATAAGATGGGATTGATTGCAAATGGTGATCT
GCGTCTACCTTTGACGCAACTTTCGGAACCAGCACGCCCAATCGTTGCCCAAGCCCTGTCTGAAGCCTGTATTTATTAA

Upstream 100 bases:

>100_bases
TCAATATTCATCCGCATGGTGATTTAAGCCCATTTCTCCGCTTGTCTTTTTTTAGCGCCATGCATTACCATGCTGAGAGT
ATCAGATTAGGGAGATAGAC

Downstream 100 bases:

>100_bases
GGCGCGATCCATTACAATGCCGGGGTATCATGACCCCGGCAATTTTTAGGAGTTTCAATGAAGTTTTCTCGCCAGCTAGT
TCTCAGTTCACTGGCTGTTT

Product: dihydrodipicolinate synthase

Products: NA

Alternate protein names: DHDPS

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MFSGSIVALITPFTPDGEVDYISLKKLVDFHVDAGTDAIVSVGTTGESATLTVEEHVKVVAKTVEFAEGRLPIIAGTGAN
ATHEAVTFSRLLNNTGIAGYLSVTPYYNKPTQEGLFLHYNAIAQETDIPVILYNVPGRTAVDMRPETVARLSEIKNIVAL
KDATGDLSRVAKHREMCKEGFVLLSGDDATGLEFVKLGGQGVISVTNNIAAADMAKMMHLALDGKFDEAASINQRLMTLH
KNLFIESSPIPVKWAAHKMGLIANGDLRLPLTQLSEPARPIVAQALSEACIY

Sequences:

>Translated_292_residues
MFSGSIVALITPFTPDGEVDYISLKKLVDFHVDAGTDAIVSVGTTGESATLTVEEHVKVVAKTVEFAEGRLPIIAGTGAN
ATHEAVTFSRLLNNTGIAGYLSVTPYYNKPTQEGLFLHYNAIAQETDIPVILYNVPGRTAVDMRPETVARLSEIKNIVAL
KDATGDLSRVAKHREMCKEGFVLLSGDDATGLEFVKLGGQGVISVTNNIAAADMAKMMHLALDGKFDEAASINQRLMTLH
KNLFIESSPIPVKWAAHKMGLIANGDLRLPLTQLSEPARPIVAQALSEACIY
>Mature_292_residues
MFSGSIVALITPFTPDGEVDYISLKKLVDFHVDAGTDAIVSVGTTGESATLTVEEHVKVVAKTVEFAEGRLPIIAGTGAN
ATHEAVTFSRLLNNTGIAGYLSVTPYYNKPTQEGLFLHYNAIAQETDIPVILYNVPGRTAVDMRPETVARLSEIKNIVAL
KDATGDLSRVAKHREMCKEGFVLLSGDDATGLEFVKLGGQGVISVTNNIAAADMAKMMHLALDGKFDEAASINQRLMTLH
KNLFIESSPIPVKWAAHKMGLIANGDLRLPLTQLSEPARPIVAQALSEACIY

Specific function: Biosynthesis of diaminopimelate and lysine from aspartate semialdehyde; first step. [C]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family

Homologues:

Organism=Homo sapiens, GI31543060, Length=276, Percent_Identity=28.2608695652174, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI13540533, Length=283, Percent_Identity=25.0883392226148, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788823, Length=289, Percent_Identity=58.1314878892734, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI87082415, Length=283, Percent_Identity=29.3286219081272, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1786463, Length=246, Percent_Identity=29.2682926829268, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789620, Length=209, Percent_Identity=29.6650717703349, Blast_Score=95, Evalue=6e-21,

Paralogues:

None

Copy number: 840 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): DAPA_VIBC3 (A5F699)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F699
- SMR:   A5F699
- STRING:   A5F699
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1738
- eggNOG:   COG0329
- HOGENOM:   HBG358848
- OMA:   GMYRHFK
- ProtClustDB:   PRK03170
- BioCyc:   VCHO345073:VC0395_A1738-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00418
- InterPro:   IPR013785
- InterPro:   IPR005263
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR020624
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR12128
- PRINTS:   PR00146
- TIGRFAMs:   TIGR00674

Pfam domain/function: PF00701 DHDPS

EC number: =4.2.1.52

Molecular weight: Translated: 31348; Mature: 31348

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00665 DHDPS_1; PS00666 DHDPS_2

Important sites: ACT_SITE 161-161 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGSIVALITPFTPDGEVDYISLKKLVDFHVDAGTDAIVSVGTTGESATLTVEEHVKVV
CCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHH
AKTVEFAEGRLPIIAGTGANATHEAVTFSRLLNNTGIAGYLSVTPYYNKPTQEGLFLHYN
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEE
AIAQETDIPVILYNVPGRTAVDMRPETVARLSEIKNIVALKDATGDLSRVAKHREMCKEG
CHHCCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCC
FVLLSGDDATGLEFVKLGGQGVISVTNNIAAADMAKMMHLALDGKFDEAASINQRLMTLH
EEEEECCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KNLFIESSPIPVKWAAHKMGLIANGDLRLPLTQLSEPARPIVAQALSEACIY
HHHEEECCCCCEEEHHHHCCEEECCCEECCHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSGSIVALITPFTPDGEVDYISLKKLVDFHVDAGTDAIVSVGTTGESATLTVEEHVKVV
CCCCCEEEEEECCCCCCCEEEEEHHHHHHHCCCCCCCEEEEECCCCCCEEEEHHHHHHHH
AKTVEFAEGRLPIIAGTGANATHEAVTFSRLLNNTGIAGYLSVTPYYNKPTQEGLFLHYN
HHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEE
AIAQETDIPVILYNVPGRTAVDMRPETVARLSEIKNIVALKDATGDLSRVAKHREMCKEG
CHHCCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHCC
FVLLSGDDATGLEFVKLGGQGVISVTNNIAAADMAKMMHLALDGKFDEAASINQRLMTLH
EEEEECCCCCCEEEEEECCCEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KNLFIESSPIPVKWAAHKMGLIANGDLRLPLTQLSEPARPIVAQALSEACIY
HHHEEECCCCCEEEHHHHCCEEECCCEECCHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA