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The map label for this gene is ubiH [H]

Identifier: 147673178

GI number: 147673178

Start: 2209017

End: 2210225

Strand: Reverse

Name: ubiH [H]

Synonym: VC0395_A2052

Alternate gene names: 147673178

Gene position: 2210225-2209017 (Counterclockwise)

Preceding gene: 147673888

Following gene: 147673773

Centisome position: 73.09

GC content: 53.1

Gene sequence:

>1209_bases
ATGACGGACAGCCAGCAGCCCAATATCAGTAAGCCTATCAGTTTTGATGTGATCATTGCGGGGGGGGCAATGGCGGGGGC
AACATTAGCGCTCGCGTTGCAGCACCTCAGCCAAGGTAAACTGAAGGTCGCCGTGATAGAGGCGTTTGCGACCGATCATT
CTGCGCATCCCGGTTTTGATGCGCGCAGCATTGCGCTCTCCTACGGTACGGTACAAATCCTGCAGGAATTACAACTTTGG
CCTAGCCTACAGCCTTTTGCGACTGCGATTAAACATATCCATGTCTCGGATCAGTCTCATGCAGGGATGACGGATATTCA
CTGTGAGACGCTTGGGGTTAGCGCTCTAGGTTATGTGGTTGAACTGGCGGATGTGGGGCGCGTTTACGCCGAGCGCATGG
CTCAGTGTTCTGCCATCCAACAATTTGTGCCAAGCCGAGTGGTGAATATTACCCGTGAACAAGAGTCGGTCACGGTCCAA
CTCAACACTAAAGAGACCTTGCAAGGTAAGCTGCTTGTGGCGGCAGATGGTGCGCTGTCGACGTGTTGCCAAGCGCTGGG
CCTTGCGATGCAGGAGCACGATTTTGAACAAGTCGCGCTGATCGCCAATATCACCAGCGCGCAACCTCATGCAGGGCGAG
CTTTTGAGCGTTTTACACCGCATGGCCCTGTTGCCTTGCTACCAATGAGCCAAGATCGCTTGTCTTTAGTTTGGTGTCTG
CCACCAGAGCAAGTTGCGCACTGGCTGCATTGCAGCGAAGCTGAGTTCTTACAAGGCTTACAACAAGCCTTTGGTTGGCG
GCTTGGCGCCTTGACCCATGCAGGTGAGAGAAGTGTTTATCCACTGGTTTTGCGCTATCGTGAGCAAACGGTCTCTCACC
GTTTTGCTATTGTGGGCAATGCGGCGCAAACCTTACACCCGATTGCAGGACAAGGTTTTAACCTTGGTATTCGTGATGTG
GCGACTTTAGCCGAAGAGATTACGCGCAGCGACGATGCGGGTGAGTACGCGCTACTGCAGCGTTATTTTCAGCGTCGCCA
AGCCGATCGCACGGCAACCATCAGCCTGACTACAGGATTAGTACACACTTTCTCGAATGACTGGTTAGCGATGCGAATGG
GGCGTAACCTTGGTCTAATGGCGATGGATAATTTGCCGTTATTGAAAGCTCCGTTATTGCGCCGCACTCTCGGCATAGTG
AACCGTTAA

Upstream 100 bases:

>100_bases
ATATCAAAGCCTGTGTATTGGTATTACATGCGGAATTTGGTGTCAAACCAGAGCAAGACACTAAGCCCACCGTTCACTAA
GTCAACAGAAAGGAAGCGCA

Downstream 100 bases:

>100_bases
TAGGTAAAAGAATGATGCAAAGCGTTGATGTTGTAATTATCGGTGGCGGTATGGTCGGCCTAGCGTTAGCGGCGGCTCTA
AAAGAGAGTGACCTACGCAT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTDSQQPNISKPISFDVIIAGGAMAGATLALALQHLSQGKLKVAVIEAFATDHSAHPGFDARSIALSYGTVQILQELQLW
PSLQPFATAIKHIHVSDQSHAGMTDIHCETLGVSALGYVVELADVGRVYAERMAQCSAIQQFVPSRVVNITREQESVTVQ
LNTKETLQGKLLVAADGALSTCCQALGLAMQEHDFEQVALIANITSAQPHAGRAFERFTPHGPVALLPMSQDRLSLVWCL
PPEQVAHWLHCSEAEFLQGLQQAFGWRLGALTHAGERSVYPLVLRYREQTVSHRFAIVGNAAQTLHPIAGQGFNLGIRDV
ATLAEEITRSDDAGEYALLQRYFQRRQADRTATISLTTGLVHTFSNDWLAMRMGRNLGLMAMDNLPLLKAPLLRRTLGIV
NR

Sequences:

>Translated_402_residues
MTDSQQPNISKPISFDVIIAGGAMAGATLALALQHLSQGKLKVAVIEAFATDHSAHPGFDARSIALSYGTVQILQELQLW
PSLQPFATAIKHIHVSDQSHAGMTDIHCETLGVSALGYVVELADVGRVYAERMAQCSAIQQFVPSRVVNITREQESVTVQ
LNTKETLQGKLLVAADGALSTCCQALGLAMQEHDFEQVALIANITSAQPHAGRAFERFTPHGPVALLPMSQDRLSLVWCL
PPEQVAHWLHCSEAEFLQGLQQAFGWRLGALTHAGERSVYPLVLRYREQTVSHRFAIVGNAAQTLHPIAGQGFNLGIRDV
ATLAEEITRSDDAGEYALLQRYFQRRQADRTATISLTTGLVHTFSNDWLAMRMGRNLGLMAMDNLPLLKAPLLRRTLGIV
NR
>Mature_401_residues
TDSQQPNISKPISFDVIIAGGAMAGATLALALQHLSQGKLKVAVIEAFATDHSAHPGFDARSIALSYGTVQILQELQLWP
SLQPFATAIKHIHVSDQSHAGMTDIHCETLGVSALGYVVELADVGRVYAERMAQCSAIQQFVPSRVVNITREQESVTVQL
NTKETLQGKLLVAADGALSTCCQALGLAMQEHDFEQVALIANITSAQPHAGRAFERFTPHGPVALLPMSQDRLSLVWCLP
PEQVAHWLHCSEAEFLQGLQQAFGWRLGALTHAGERSVYPLVLRYREQTVSHRFAIVGNAAQTLHPIAGQGFNLGIRDVA
TLAEEITRSDDAGEYALLQRYFQRRQADRTATISLTTGLVHTFSNDWLAMRMGRNLGLMAMDNLPLLKAPLLRRTLGIVN
R

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=276, Percent_Identity=29.3478260869565, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI32967307, Length=276, Percent_Identity=29.3478260869565, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789274, Length=392, Percent_Identity=51.530612244898, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1789273, Length=393, Percent_Identity=32.0610687022901, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786883, Length=389, Percent_Identity=29.8200514138817, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25146631, Length=432, Percent_Identity=25, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920760, Length=433, Percent_Identity=24.4803695150115, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.-

Molecular weight: Translated: 43939; Mature: 43808

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDSQQPNISKPISFDVIIAGGAMAGATLALALQHLSQGKLKVAVIEAFATDHSAHPGFD
CCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCCC
ARSIALSYGTVQILQELQLWPSLQPFATAIKHIHVSDQSHAGMTDIHCETLGVSALGYVV
CHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCCCCEEEHHHHCHHHHHHHH
ELADVGRVYAERMAQCSAIQQFVPSRVVNITREQESVTVQLNTKETLQGKLLVAADGALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHCCCCEEEEEECCHHH
TCCQALGLAMQEHDFEQVALIANITSAQPHAGRAFERFTPHGPVALLPMSQDRLSLVWCL
HHHHHHCCHHHHCCHHHEEEEEECCCCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEEC
PPEQVAHWLHCSEAEFLQGLQQAFGWRLGALTHAGERSVYPLVLRYREQTVSHRFAIVGN
CHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEECC
AAQTLHPIAGQGFNLGIRDVATLAEEITRSDDAGEYALLQRYFQRRQADRTATISLTTGL
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHH
VHTFSNDWLAMRMGRNLGLMAMDNLPLLKAPLLRRTLGIVNR
HHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDSQQPNISKPISFDVIIAGGAMAGATLALALQHLSQGKLKVAVIEAFATDHSAHPGFD
CCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCCC
ARSIALSYGTVQILQELQLWPSLQPFATAIKHIHVSDQSHAGMTDIHCETLGVSALGYVV
CHHEEEHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCCCCCCEEEHHHHCHHHHHHHH
ELADVGRVYAERMAQCSAIQQFVPSRVVNITREQESVTVQLNTKETLQGKLLVAADGALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCHHCCCCEEEEEECCHHH
TCCQALGLAMQEHDFEQVALIANITSAQPHAGRAFERFTPHGPVALLPMSQDRLSLVWCL
HHHHHHCCHHHHCCHHHEEEEEECCCCCCCCCCHHHHCCCCCCEEEEECCCCCEEEEEEC
PPEQVAHWLHCSEAEFLQGLQQAFGWRLGALTHAGERSVYPLVLRYREQTVSHRFAIVGN
CHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEECC
AAQTLHPIAGQGFNLGIRDVATLAEEITRSDDAGEYALLQRYFQRRQADRTATISLTTGL
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHH
VHTFSNDWLAMRMGRNLGLMAMDNLPLLKAPLLRRTLGIVNR
HHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]