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The map label for this gene is ilvI [H]

Identifier: 146342406

GI number: 146342406

Start: 5749241

End: 5751010

Strand: Reverse

Name: ilvI [H]

Synonym: BRADO5564

Alternate gene names: 146342406

Gene position: 5751010-5749241 (Counterclockwise)

Preceding gene: 146342407

Following gene: 146342405

Centisome position: 77.13

GC content: 65.76

Gene sequence:

>1770_bases
ATGACCGACAAGAGCCACGATCCGAACCAGATGACCGGCGCCGCGATGATCGTGCGCGCGCTCATCGACCACGGCGTGCA
GCACATCTTCGGCTATCCCGGCGGCGCAGTGCTTCCGATCTATGACGAGATCTTCCAGCAAAGCGAGGTCGAGCACATCC
TGGTCCGCCACGAGCAGGGCGCCGGCCATGCCGCCGAGGGCTATGCGCGCTCGACCGGCAAGCCGGGCGTCGTGCTGGTG
ACGTCGGGCCCCGGCGCCACCAATATGGTGACGCCGCTGGCGGACGCGATGATGGATTCGATCCCGCTGGTCTGCATCAC
CGGCCAGGTGCCGACGCATCTGATCGGCAACGACGCGTTCCAGGAGTGCGACACCGTCGGCATCACTCGGCCCTGCACCA
AGCACAATTGGCTGGTCCGCAATGTCAACGACCTGGCCAAGGTGCTGCACGAGGCGTTCTATGTCGCGACGTCGGGCCGG
CCGGGCCCGGTCGTCGTCGACGTGCCGAAGGACGTGCAGTTCGCCACCGGCACCTATCATCCGCCGCGCAAGTCCGACGT
CCACATCTCCTACGCGCCGCGCGTCAAGGGCGACGCGATGCAGATCCGCAAGGCCGTCGCGCTCTTGGCCTCCGCCAAGC
GGCCGGTGATCTATTCAGGCGGCGGCGTCATCAATTCGGGGCCTGACGCGTCGCGGTTGCTGCGCGAGCTGGTCGAGGTC
ACCGGCTTTCCGATCACCTCGACCCTGATGGGGCTGGGCGCCTATCCGGCGTCGGGTCCCAATTGGCTGGGCATGCTGGG
CATGCACGGCACCTACGAGGCCAACATGACGATGCATGATTGCGACGTCATGCTGTGCGTCGGCGCGCGCTTCGACGACC
GCATCACCGGCCGCACCGATGCGTTCTCGCCGAACTCGAAGAAGATCCACATCGACATCGATCCGTCCTCGATCAACAAG
AACATCCGCGTCGACGTCCCGATCATCGGCGATTGCGGCGACGTGCTCGCCGACATCCTGCAGGTGTTCAAGGCGGAAGC
GAAGAAGCCGGACATCAAGGCGTGGTGGCAGCAGATCGCGACCTGGCGCGCGCGCAACTCGCTCGCCTACAAGCCGAACC
GCGACGTCATCATGCCGCAATATGCGATCCAGCGGCTGTTCGAACTGACGCGCGGGCGCGACACCTACATCACCACCGAG
GTCGGCCAGCACCAGATGTGGGCGGCCCAGTTCTACGGCTTCGAGGAGCCGCACCGCTGGATGACCTCGGGCGGCCTCGG
CACCATGGGCTACGGCCTGCCGGCGGCGATCGGCGTGCAGGTCGCGCATCCCGACAGCCTGGTCATCGACATCGGCGGCG
ACGCCTCGGTGCAGATGACGATGCAGGAGATGTCGACGGCCGTGCAGTACGAGCTGCCGGTCAAGATCTTCATCCTGAAC
AACCAGTACATGGGCATGGTGCGGCAGTGGCAGCAGCTCCTGCACGGCAATCGCCTGTCGCATTCCTACTCGGAGGCGCT
GCCGGACTTCGTCAAGCTCGCCGAGGCTTATGGCGGCGTTGGGTTGCAGGTGAGCAAGCCCGGTGATCTCGACGGCGCGA
TCAAGGAGATGATCTCGGTCAAGAAGCCGGTGCTGTTCGACTGCCGCGTTGCTGCGCTCGAGAACTGCTTCCCGATGATC
CCGTCGGGCAAGGCGCACAACGAGATGATCCTGCCGGCCGAGGCGTCCGATCCCGCCGCCGCGTTCGCGGGCGGCAAGGC
GCTGGTGTGA

Upstream 100 bases:

>100_bases
GCAGGGCCTTCCAGGGCCCTTTTTTATTGCCCGAACCGACCCCGACCGAACCGAAAGACCTAACGACAAGCCGCTGAGAC
ACAGCGATCCGGAGCCCACC

Downstream 100 bases:

>100_bases
GGACGGATCGTGGGCATAACTCGCTCGACACACTCCGCTGTCATCGCCCGGCTTGACCGGGCGATCCAGTACGCCGCGGC
CGTGGTGAGTCCCACGACCG

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 589; Mature: 588

Protein sequence:

>589_residues
MTDKSHDPNQMTGAAMIVRALIDHGVQHIFGYPGGAVLPIYDEIFQQSEVEHILVRHEQGAGHAAEGYARSTGKPGVVLV
TSGPGATNMVTPLADAMMDSIPLVCITGQVPTHLIGNDAFQECDTVGITRPCTKHNWLVRNVNDLAKVLHEAFYVATSGR
PGPVVVDVPKDVQFATGTYHPPRKSDVHISYAPRVKGDAMQIRKAVALLASAKRPVIYSGGGVINSGPDASRLLRELVEV
TGFPITSTLMGLGAYPASGPNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRTDAFSPNSKKIHIDIDPSSINK
NIRVDVPIIGDCGDVLADILQVFKAEAKKPDIKAWWQQIATWRARNSLAYKPNRDVIMPQYAIQRLFELTRGRDTYITTE
VGQHQMWAAQFYGFEEPHRWMTSGGLGTMGYGLPAAIGVQVAHPDSLVIDIGGDASVQMTMQEMSTAVQYELPVKIFILN
NQYMGMVRQWQQLLHGNRLSHSYSEALPDFVKLAEAYGGVGLQVSKPGDLDGAIKEMISVKKPVLFDCRVAALENCFPMI
PSGKAHNEMILPAEASDPAAAFAGGKALV

Sequences:

>Translated_589_residues
MTDKSHDPNQMTGAAMIVRALIDHGVQHIFGYPGGAVLPIYDEIFQQSEVEHILVRHEQGAGHAAEGYARSTGKPGVVLV
TSGPGATNMVTPLADAMMDSIPLVCITGQVPTHLIGNDAFQECDTVGITRPCTKHNWLVRNVNDLAKVLHEAFYVATSGR
PGPVVVDVPKDVQFATGTYHPPRKSDVHISYAPRVKGDAMQIRKAVALLASAKRPVIYSGGGVINSGPDASRLLRELVEV
TGFPITSTLMGLGAYPASGPNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRTDAFSPNSKKIHIDIDPSSINK
NIRVDVPIIGDCGDVLADILQVFKAEAKKPDIKAWWQQIATWRARNSLAYKPNRDVIMPQYAIQRLFELTRGRDTYITTE
VGQHQMWAAQFYGFEEPHRWMTSGGLGTMGYGLPAAIGVQVAHPDSLVIDIGGDASVQMTMQEMSTAVQYELPVKIFILN
NQYMGMVRQWQQLLHGNRLSHSYSEALPDFVKLAEAYGGVGLQVSKPGDLDGAIKEMISVKKPVLFDCRVAALENCFPMI
PSGKAHNEMILPAEASDPAAAFAGGKALV
>Mature_588_residues
TDKSHDPNQMTGAAMIVRALIDHGVQHIFGYPGGAVLPIYDEIFQQSEVEHILVRHEQGAGHAAEGYARSTGKPGVVLVT
SGPGATNMVTPLADAMMDSIPLVCITGQVPTHLIGNDAFQECDTVGITRPCTKHNWLVRNVNDLAKVLHEAFYVATSGRP
GPVVVDVPKDVQFATGTYHPPRKSDVHISYAPRVKGDAMQIRKAVALLASAKRPVIYSGGGVINSGPDASRLLRELVEVT
GFPITSTLMGLGAYPASGPNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRTDAFSPNSKKIHIDIDPSSINKN
IRVDVPIIGDCGDVLADILQVFKAEAKKPDIKAWWQQIATWRARNSLAYKPNRDVIMPQYAIQRLFELTRGRDTYITTEV
GQHQMWAAQFYGFEEPHRWMTSGGLGTMGYGLPAAIGVQVAHPDSLVIDIGGDASVQMTMQEMSTAVQYELPVKIFILNN
QYMGMVRQWQQLLHGNRLSHSYSEALPDFVKLAEAYGGVGLQVSKPGDLDGAIKEMISVKKPVLFDCRVAALENCFPMIP
SGKAHNEMILPAEASDPAAAFAGGKALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=555, Percent_Identity=25.7657657657658, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI21361361, Length=566, Percent_Identity=24.5583038869258, Blast_Score=150, Evalue=4e-36,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=52.0210896309315, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1790104, Length=565, Percent_Identity=43.0088495575221, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=35.2836879432624, Blast_Score=308, Evalue=8e-85,
Organism=Escherichia coli, GI1787096, Length=558, Percent_Identity=29.3906810035842, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI1788716, Length=561, Percent_Identity=27.807486631016, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=26.1180679785331, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=26.1180679785331, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=503, Percent_Identity=26.6401590457256, Blast_Score=128, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=580, Percent_Identity=47.0689655172414, Blast_Score=511, Evalue=1e-145,
Organism=Saccharomyces cerevisiae, GI6320816, Length=492, Percent_Identity=23.9837398373984, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922626, Length=485, Percent_Identity=26.8041237113402, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63997; Mature: 63866

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKSHDPNQMTGAAMIVRALIDHGVQHIFGYPGGAVLPIYDEIFQQSEVEHILVRHEQG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCHHEEEEEECCC
AGHAAEGYARSTGKPGVVLVTSGPGATNMVTPLADAMMDSIPLVCITGQVPTHLIGNDAF
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHCCCHHH
QECDTVGITRPCTKHNWLVRNVNDLAKVLHEAFYVATSGRPGPVVVDVPKDVQFATGTYH
HHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEECCCCCC
PPRKSDVHISYAPRVKGDAMQIRKAVALLASAKRPVIYSGGGVINSGPDASRLLRELVEV
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHH
TGFPITSTLMGLGAYPASGPNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRTD
CCCCHHHHHHHCCCCCCCCCCCEEEECCCCEEECCCEEECCCEEEEECCCCCCCCCCCCC
AFSPNSKKIHIDIDPSSINKNIRVDVPIIGDCGDVLADILQVFKAEAKKPDIKAWWQQIA
CCCCCCCEEEEEECHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TWRARNSLAYKPNRDVIMPQYAIQRLFELTRGRDTYITTEVGQHQMWAAQFYGFEEPHRW
HHHHCCCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHH
MTSGGLGTMGYGLPAAIGVQVAHPDSLVIDIGGDASVQMTMQEMSTAVQYELPVKIFILN
HHCCCCCCCCCCCCHHHEEEEECCCEEEEECCCCCEEEEEHHHHHHHEEEECCEEEEEEC
NQYMGMVRQWQQLLHGNRLSHSYSEALPDFVKLAEAYGGVGLQVSKPGDLDGAIKEMISV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
KKPVLFDCRVAALENCFPMIPSGKAHNEMILPAEASDPAAAFAGGKALV
CCCCEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
TDKSHDPNQMTGAAMIVRALIDHGVQHIFGYPGGAVLPIYDEIFQQSEVEHILVRHEQG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCHHEEEEEECCC
AGHAAEGYARSTGKPGVVLVTSGPGATNMVTPLADAMMDSIPLVCITGQVPTHLIGNDAF
CCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHCCCHHH
QECDTVGITRPCTKHNWLVRNVNDLAKVLHEAFYVATSGRPGPVVVDVPKDVQFATGTYH
HHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHEEEECCCCCCEEEECCCCCEECCCCCC
PPRKSDVHISYAPRVKGDAMQIRKAVALLASAKRPVIYSGGGVINSGPDASRLLRELVEV
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHH
TGFPITSTLMGLGAYPASGPNWLGMLGMHGTYEANMTMHDCDVMLCVGARFDDRITGRTD
CCCCHHHHHHHCCCCCCCCCCCEEEECCCCEEECCCEEECCCEEEEECCCCCCCCCCCCC
AFSPNSKKIHIDIDPSSINKNIRVDVPIIGDCGDVLADILQVFKAEAKKPDIKAWWQQIA
CCCCCCCEEEEEECHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TWRARNSLAYKPNRDVIMPQYAIQRLFELTRGRDTYITTEVGQHQMWAAQFYGFEEPHRW
HHHHCCCCCCCCCCCEECHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCCCHHH
MTSGGLGTMGYGLPAAIGVQVAHPDSLVIDIGGDASVQMTMQEMSTAVQYELPVKIFILN
HHCCCCCCCCCCCCHHHEEEEECCCEEEEECCCCCEEEEEHHHHHHHEEEECCEEEEEEC
NQYMGMVRQWQQLLHGNRLSHSYSEALPDFVKLAEAYGGVGLQVSKPGDLDGAIKEMISV
CHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHH
KKPVLFDCRVAALENCFPMIPSGKAHNEMILPAEASDPAAAFAGGKALV
CCCCEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]