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The map label for this gene is nifE [H]

Identifier: 146342283

GI number: 146342283

Start: 5622691

End: 5624364

Strand: Reverse

Name: nifE [H]

Synonym: BRADO5437

Alternate gene names: 146342283

Gene position: 5624364-5622691 (Counterclockwise)

Preceding gene: 146342284

Following gene: 146342282

Centisome position: 75.43

GC content: 65.53

Gene sequence:

>1674_bases
ATGAGCTCGCTGTCTGCCACGATCCAGAACGTCTTCAACGAGCCGGGTTGCGGCAAGAACGCCAACAAGTCCGAGGCCGA
GCGCAAGAAGGGCTGCACCAAGCAGCTGCAGCCGGGCGGCGCGGCCGGTGGCTGCGCCTTCGACGGCGCCAAGGTCGCGT
TGCAGCCGCTGACCGACGTCGCGCATCTCGTGCACGGGCCGATCGCCTGCGAAGGAAATTCCTGGGACAACCGCGGTGCG
GCCTCGTCTGGCTCGCAGATCTGGCGCACCGGTTTCACCACCGATATCAACGAGACCGACGTCGTGTTCGGCGGCGAGAA
GCGCCTGTACAAGGCGATCAAGGAAATCATCGAGAAATACGATCCGCCGGCGGTCTTCGTCTACCAGACCTGCGTGCCCG
CCATGACCGGCGACGACATCAACGCGGTCTGCAAGGCGGCCTCGGCCAAGTTCGGCAAGCCCTGCATTCCCGTCAATTCG
CCGGGCTTCGTCGGTCCGAAGAACCTCGGCAACAAGCTCGCAGGCGAGGCGCTGCTCGACCACGTCATCGGCACCGTCGA
GCCGGAGGTCACGACGCCCTACGATCTCAACATCATCGGCGAATACAATCTCTCGGGCGAGCTCTGGCAGGTGAAGCCGC
TGTTCGACGAACTCGGCATCCGCATCTCCGCCTGCATCTCGGGCGACGGCCGCTACAAGGACGTCGCGTCGTCGCATCGC
GCTCGCGCGGCGATGATGGTGTGCTCCAAGGCGATGATCAACGTCGCCCGCAAGATGGAGGAGCGCTACGGCATTCCGTT
CTTCGAGGGCTCGTTCTACGGCATCCAGGACTCCTCGGACTCGCTGCGCGAAATCGCACGCCTGCTGGTCGAGCGCGGCG
CGCCGGCCGACCTGCTGGACCGCACCGAGGCGGTGATCGCGCGCGAGGAGGCCAAGGCCTGGGCGGCGATCGAGCCGTTC
AAGAAGCGGCTGACCGGCAAGAAGGTGCTGCTGATCACCGGCGGCGTGAAGTCATGGTCGGTGGTGGCGGCGCTGCAGGA
AGCCGGCATGGAGTTGGTCGGCACCTCCGTGAAGAAGTCCACCAAGGAGGACAAGGAGCGCATCAAGGAGCTGATGGGCC
AGGACGCCCACATGATCGACGATATGGCGCCCCGCGAGATGTACAAGATGCTCAAGGACGCCAAGGCCGACATCATGCTG
TCCGGCGGCAAGTCGCAATTCGTGGCGCTGAAGGCCGCGATGCCCTGGCTCGACATCAACCAGGAGCGCTCGCACGCCTT
CATGGGCTATATCGGCATGGTGAAGCTGGTCGCCGAGATCGACAAAGCGATCTACAACCCGATGTGGGAGCAGCTGCGCA
AGCGGGCGCCCTGGGAGACGGCCAGCAACAACTGGCAGGCCAAGGCGATCGCGCAGGCCAATGCAGAAGCCGCGGCGCTC
GCCGCCGATCCCGTCGCTGCCGAGGCCGCGCGCCGCGCCAAGAAGATCTGCCACTGCAAGTCGGTCGATCTCGGTACCAT
CGAGGATGCGATCAAGGCGCATGGTCTAACCGACGTCGCCGGCGTTCGTACCCACACCAACGCCTCGGGCGGCTGCGGCG
CCTGTGCCGGTCGGGTCGAGGATATCCTGGCGGCGCAGAAGGCGCCCGCCGAGCTGCTGCAGGCAGCGGAATAG

Upstream 100 bases:

>100_bases
CCGCGCTTCGCTCCAGACGATGCGGCTCGTGGGAAGGCACCAAGCCTGAGCCGCCTCGTCCGACCAAGACGCGCAGAAGG
TCCGCCAACAGGAGAAGCCG

Downstream 100 bases:

>100_bases
GGTGGCGCTCATGGCCAAGGTCACCATCGGAAAGAAGGCCTGCGCGGTCAATCCGCTGAAGATGAGCCAGCCGATCGGCG
GCTCGTTCGCCTTCCTCGGC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSSLSATIQNVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKVALQPLTDVAHLVHGPIACEGNSWDNRGA
ASSGSQIWRTGFTTDINETDVVFGGEKRLYKAIKEIIEKYDPPAVFVYQTCVPAMTGDDINAVCKAASAKFGKPCIPVNS
PGFVGPKNLGNKLAGEALLDHVIGTVEPEVTTPYDLNIIGEYNLSGELWQVKPLFDELGIRISACISGDGRYKDVASSHR
ARAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIQDSSDSLREIARLLVERGAPADLLDRTEAVIAREEAKAWAAIEPF
KKRLTGKKVLLITGGVKSWSVVAALQEAGMELVGTSVKKSTKEDKERIKELMGQDAHMIDDMAPREMYKMLKDAKADIML
SGGKSQFVALKAAMPWLDINQERSHAFMGYIGMVKLVAEIDKAIYNPMWEQLRKRAPWETASNNWQAKAIAQANAEAAAL
AADPVAAEAARRAKKICHCKSVDLGTIEDAIKAHGLTDVAGVRTHTNASGGCGACAGRVEDILAAQKAPAELLQAAE

Sequences:

>Translated_557_residues
MSSLSATIQNVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKVALQPLTDVAHLVHGPIACEGNSWDNRGA
ASSGSQIWRTGFTTDINETDVVFGGEKRLYKAIKEIIEKYDPPAVFVYQTCVPAMTGDDINAVCKAASAKFGKPCIPVNS
PGFVGPKNLGNKLAGEALLDHVIGTVEPEVTTPYDLNIIGEYNLSGELWQVKPLFDELGIRISACISGDGRYKDVASSHR
ARAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIQDSSDSLREIARLLVERGAPADLLDRTEAVIAREEAKAWAAIEPF
KKRLTGKKVLLITGGVKSWSVVAALQEAGMELVGTSVKKSTKEDKERIKELMGQDAHMIDDMAPREMYKMLKDAKADIML
SGGKSQFVALKAAMPWLDINQERSHAFMGYIGMVKLVAEIDKAIYNPMWEQLRKRAPWETASNNWQAKAIAQANAEAAAL
AADPVAAEAARRAKKICHCKSVDLGTIEDAIKAHGLTDVAGVRTHTNASGGCGACAGRVEDILAAQKAPAELLQAAE
>Mature_556_residues
SSLSATIQNVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKVALQPLTDVAHLVHGPIACEGNSWDNRGAA
SSGSQIWRTGFTTDINETDVVFGGEKRLYKAIKEIIEKYDPPAVFVYQTCVPAMTGDDINAVCKAASAKFGKPCIPVNSP
GFVGPKNLGNKLAGEALLDHVIGTVEPEVTTPYDLNIIGEYNLSGELWQVKPLFDELGIRISACISGDGRYKDVASSHRA
RAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIQDSSDSLREIARLLVERGAPADLLDRTEAVIAREEAKAWAAIEPFK
KRLTGKKVLLITGGVKSWSVVAALQEAGMELVGTSVKKSTKEDKERIKELMGQDAHMIDDMAPREMYKMLKDAKADIMLS
GGKSQFVALKAAMPWLDINQERSHAFMGYIGMVKLVAEIDKAIYNPMWEQLRKRAPWETASNNWQAKAIAQANAEAAALA
ADPVAAEAARRAKKICHCKSVDLGTIEDAIKAHGLTDVAGVRTHTNASGGCGACAGRVEDILAAQKAPAELLQAAE

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 59889; Mature: 59758

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLSATIQNVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKVALQPLTDV
CCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHGPIACEGNSWDNRGAASSGSQIWRTGFTTDINETDVVFGGEKRLYKAIKEIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
DPPAVFVYQTCVPAMTGDDINAVCKAASAKFGKPCIPVNSPGFVGPKNLGNKLAGEALLD
CCCEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HVIGTVEPEVTTPYDLNIIGEYNLSGELWQVKPLFDELGIRISACISGDGRYKDVASSHR
HHHHCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHCCCEEEEEECCCCCHHHHHHHHH
ARAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIQDSSDSLREIARLLVERGAPADLLD
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCCHHHHH
RTEAVIAREEAKAWAAIEPFKKRLTGKKVLLITGGVKSWSVVAALQEAGMELVGTSVKKS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
TKEDKERIKELMGQDAHMIDDMAPREMYKMLKDAKADIMLSGGKSQFVALKAAMPWLDIN
HHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCEEEEEHHCCCCCCC
QERSHAFMGYIGMVKLVAEIDKAIYNPMWEQLRKRAPWETASNNWQAKAIAQANAEAAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHH
AADPVAAEAARRAKKICHCKSVDLGTIEDAIKAHGLTDVAGVRTHTNASGGCGACAGRVE
HCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHH
DILAAQKAPAELLQAAE
HHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SSLSATIQNVFNEPGCGKNANKSEAERKKGCTKQLQPGGAAGGCAFDGAKVALQPLTDV
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHCCCCCCCCCCCCCCCHHHHHHHHHH
AHLVHGPIACEGNSWDNRGAASSGSQIWRTGFTTDINETDVVFGGEKRLYKAIKEIIEKY
HHHHCCCEEECCCCCCCCCCCCCCCHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHC
DPPAVFVYQTCVPAMTGDDINAVCKAASAKFGKPCIPVNSPGFVGPKNLGNKLAGEALLD
CCCEEEEEHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
HVIGTVEPEVTTPYDLNIIGEYNLSGELWQVKPLFDELGIRISACISGDGRYKDVASSHR
HHHHCCCCCCCCCCCEEEEEEECCCCCEEEECHHHHHCCCEEEEEECCCCCHHHHHHHHH
ARAAMMVCSKAMINVARKMEERYGIPFFEGSFYGIQDSSDSLREIARLLVERGAPADLLD
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEECCCCCHHHHHHHHHHHHHCCCCHHHHH
RTEAVIAREEAKAWAAIEPFKKRLTGKKVLLITGGVKSWSVVAALQEAGMELVGTSVKKS
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHH
TKEDKERIKELMGQDAHMIDDMAPREMYKMLKDAKADIMLSGGKSQFVALKAAMPWLDIN
HHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHCCCCCEEEECCCCCEEEEEHHCCCCCCC
QERSHAFMGYIGMVKLVAEIDKAIYNPMWEQLRKRAPWETASNNWQAKAIAQANAEAAAL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHH
AADPVAAEAARRAKKICHCKSVDLGTIEDAIKAHGLTDVAGVRTHTNASGGCGACAGRVE
HCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHH
DILAAQKAPAELLQAAE
HHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]