Click here to switch to the map view.

The map label for this gene is nifN [H]

Identifier: 146342282

GI number: 146342282

Start: 5621283

End: 5622680

Strand: Reverse

Name: nifN [H]

Synonym: BRADO5436

Alternate gene names: 146342282

Gene position: 5622680-5621283 (Counterclockwise)

Preceding gene: 146342283

Following gene: 146342281

Centisome position: 75.41

GC content: 65.88

Gene sequence:

>1398_bases
ATGGCCAAGGTCACCATCGGAAAGAAGGCCTGCGCGGTCAATCCGCTGAAGATGAGCCAGCCGATCGGCGGCTCGTTCGC
CTTCCTCGGCCTGCGCGGCGCGATGCCGCTGCTGCACGGTTCGCAGGGCTGCACCTCGTTCGGCCTCACCCTGTTCGTGC
GCCACTTCAAGGAAGCGGTGCCCATGCAGACGACGGCCATGAGCGAGGTCGCGACCGTGCTCGGCGGATACGAGAATGTC
GAGCAGGCGATCCTCAACATCTACAAGAAGCAGAAGCCGGAGATCATCGGCATCTGCTCCACGGGCGTCACCGAGACCAA
GGGCGACGACGTCGACGGCTACATCAAGCTGATCCGCGAGAAGCACCCGGAGCTCGCCAATTATCCGCTGGTCTACGTCT
CGACCCCGGACTTCAAGGATGCGTTCCAGGACGGCTGGGACAAGACGGTTGCCAAGATGGTCGAGGTGCTCGTCGACAAG
CCGACCTCGACCCGGCGCGATCCTGCCAGGGTCAACGTGCTGCCGGGCTGCCATCTGACGCCCGGCGATATCGAGGAGCT
CCGCACCATCTTCGAGGATTTCGGCCTGGAGCCGTCGTTCCTGCCGGATATCGGCGGCTCGCTCGACGGCCACATCCCCG
ACGAGTTCACGCCGACCACGATCGGCGGCATCGGCCTCGAAGAGATCGCGACCATGGGCAGCGCGTCCTGGACGATCGCG
ATCGGCGCGCAGATGAAGCGCGCGGCGGAAGCGATGCAGGCGAAGACCGGCGTGCCGTACCGGCTGTTCGAGCGGCTCTG
CGGCCTGCTGCCGAACGACGAGTTCATGGCCTTCCTGAGCGAGATCTCGGGGCGTCCGGTGCCGGCGAAATATCGCCGCC
AGCGCGGCCAGCTCGCGGATGCGATGCTGGATGCGCATTTCCATATCGGCGGCCGCAAGCTCGCGATCGGCGCCGAGCCG
GATTTGCTGTTCGACGTCGCCAGCATGCTGCACGACATGGGCGCCGAGATCGCGGTCGCCGTCACCACCACGCAGTCGCC
GGTGCTGGAGCGCATGCCGTGCGAGGAGGTGCTGATCGGCGATCTCGAGGATCTGGAGACGCGCGCAAAAGAGCGCGACT
GCGGTCTGATGATGACGCATTCGCACGGCCGCCAGGCGGCGGCGCGGCTCAAGATCCCGTTCTACCGGATCGGCTTTCCG
ATGTTCGACCGGCTGGGTGCTGGGCACCTGTTGACCGTCGGCTATCGCGGCACCCGCGACCTGGTGTTTGCGCTCGCCAA
CCTGATCATCGCCGACCGCGAGGACAACCATCAGCCGACGCCGGACACCTGGATCGATCCCTGGAAGGATTTCCAGCAGG
CCTCGACCGAGTCCGCCGTCGCGGCGCCGTTGCATTGA

Upstream 100 bases:

>100_bases
TCGGGCGGCTGCGGCGCCTGTGCCGGTCGGGTCGAGGATATCCTGGCGGCGCAGAAGGCGCCCGCCGAGCTGCTGCAGGC
AGCGGAATAGGGTGGCGCTC

Downstream 100 bases:

>100_bases
CGCTCGAGGAGAAAGGACCGACGACATGAAAGTCGCATTCGCTACCCAGGATCTGAAACGCGTCGACGCCCATTTCGGCT
GGGCCAAGAACATCGCGATC

Product: nitrogenase molybdenum-cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MAKVTIGKKACAVNPLKMSQPIGGSFAFLGLRGAMPLLHGSQGCTSFGLTLFVRHFKEAVPMQTTAMSEVATVLGGYENV
EQAILNIYKKQKPEIIGICSTGVTETKGDDVDGYIKLIREKHPELANYPLVYVSTPDFKDAFQDGWDKTVAKMVEVLVDK
PTSTRRDPARVNVLPGCHLTPGDIEELRTIFEDFGLEPSFLPDIGGSLDGHIPDEFTPTTIGGIGLEEIATMGSASWTIA
IGAQMKRAAEAMQAKTGVPYRLFERLCGLLPNDEFMAFLSEISGRPVPAKYRRQRGQLADAMLDAHFHIGGRKLAIGAEP
DLLFDVASMLHDMGAEIAVAVTTTQSPVLERMPCEEVLIGDLEDLETRAKERDCGLMMTHSHGRQAAARLKIPFYRIGFP
MFDRLGAGHLLTVGYRGTRDLVFALANLIIADREDNHQPTPDTWIDPWKDFQQASTESAVAAPLH

Sequences:

>Translated_465_residues
MAKVTIGKKACAVNPLKMSQPIGGSFAFLGLRGAMPLLHGSQGCTSFGLTLFVRHFKEAVPMQTTAMSEVATVLGGYENV
EQAILNIYKKQKPEIIGICSTGVTETKGDDVDGYIKLIREKHPELANYPLVYVSTPDFKDAFQDGWDKTVAKMVEVLVDK
PTSTRRDPARVNVLPGCHLTPGDIEELRTIFEDFGLEPSFLPDIGGSLDGHIPDEFTPTTIGGIGLEEIATMGSASWTIA
IGAQMKRAAEAMQAKTGVPYRLFERLCGLLPNDEFMAFLSEISGRPVPAKYRRQRGQLADAMLDAHFHIGGRKLAIGAEP
DLLFDVASMLHDMGAEIAVAVTTTQSPVLERMPCEEVLIGDLEDLETRAKERDCGLMMTHSHGRQAAARLKIPFYRIGFP
MFDRLGAGHLLTVGYRGTRDLVFALANLIIADREDNHQPTPDTWIDPWKDFQQASTESAVAAPLH
>Mature_464_residues
AKVTIGKKACAVNPLKMSQPIGGSFAFLGLRGAMPLLHGSQGCTSFGLTLFVRHFKEAVPMQTTAMSEVATVLGGYENVE
QAILNIYKKQKPEIIGICSTGVTETKGDDVDGYIKLIREKHPELANYPLVYVSTPDFKDAFQDGWDKTVAKMVEVLVDKP
TSTRRDPARVNVLPGCHLTPGDIEELRTIFEDFGLEPSFLPDIGGSLDGHIPDEFTPTTIGGIGLEEIATMGSASWTIAI
GAQMKRAAEAMQAKTGVPYRLFERLCGLLPNDEFMAFLSEISGRPVPAKYRRQRGQLADAMLDAHFHIGGRKLAIGAEPD
LLFDVASMLHDMGAEIAVAVTTTQSPVLERMPCEEVLIGDLEDLETRAKERDCGLMMTHSHGRQAAARLKIPFYRIGFPM
FDRLGAGHLLTVGYRGTRDLVFALANLIIADREDNHQPTPDTWIDPWKDFQQASTESAVAAPLH

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005975 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 50896; Mature: 50765

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVTIGKKACAVNPLKMSQPIGGSFAFLGLRGAMPLLHGSQGCTSFGLTLFVRHFKEAV
CCEEECCCHHHCCCCHHCCCCCCCCEEEEEHHCCCHHHCCCCCCHHHHHHHHHHHHHHHC
PMQTTAMSEVATVLGGYENVEQAILNIYKKQKPEIIGICSTGVTETKGDDVDGYIKLIRE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KHPELANYPLVYVSTPDFKDAFQDGWDKTVAKMVEVLVDKPTSTRRDPARVNVLPGCHLT
HCCCHHCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
PGDIEELRTIFEDFGLEPSFLPDIGGSLDGHIPDEFTPTTIGGIGLEEIATMGSASWTIA
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEE
IGAQMKRAAEAMQAKTGVPYRLFERLCGLLPNDEFMAFLSEISGRPVPAKYRRQRGQLAD
ECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AMLDAHFHIGGRKLAIGAEPDLLFDVASMLHDMGAEIAVAVTTTQSPVLERMPCEEVLIG
HHHHHHEEECCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCHHHHHHC
DLEDLETRAKERDCGLMMTHSHGRQAAARLKIPFYRIGFPMFDRLGAGHLLTVGYRGTRD
CHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCHHCCCCHHHHHCCCCCEEEECCCCHHH
LVFALANLIIADREDNHQPTPDTWIDPWKDFQQASTESAVAAPLH
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AKVTIGKKACAVNPLKMSQPIGGSFAFLGLRGAMPLLHGSQGCTSFGLTLFVRHFKEAV
CEEECCCHHHCCCCHHCCCCCCCCEEEEEHHCCCHHHCCCCCCHHHHHHHHHHHHHHHC
PMQTTAMSEVATVLGGYENVEQAILNIYKKQKPEIIGICSTGVTETKGDDVDGYIKLIRE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KHPELANYPLVYVSTPDFKDAFQDGWDKTVAKMVEVLVDKPTSTRRDPARVNVLPGCHLT
HCCCHHCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
PGDIEELRTIFEDFGLEPSFLPDIGGSLDGHIPDEFTPTTIGGIGLEEIATMGSASWTIA
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCEEEE
IGAQMKRAAEAMQAKTGVPYRLFERLCGLLPNDEFMAFLSEISGRPVPAKYRRQRGQLAD
ECHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AMLDAHFHIGGRKLAIGAEPDLLFDVASMLHDMGAEIAVAVTTTQSPVLERMPCEEVLIG
HHHHHHEEECCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHCCHHHHHHC
DLEDLETRAKERDCGLMMTHSHGRQAAARLKIPFYRIGFPMFDRLGAGHLLTVGYRGTRD
CHHHHHHHHHHCCCCEEEECCCCHHHHHHCCCCHHCCCCHHHHHCCCCCEEEECCCCHHH
LVFALANLIIADREDNHQPTPDTWIDPWKDFQQASTESAVAAPLH
HHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2266945; 11157954; 12597275 [H]