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The map label for this gene is nifK [H]

Identifier: 146342284

GI number: 146342284

Start: 5624495

End: 5626054

Strand: Reverse

Name: nifK [H]

Synonym: BRADO5438

Alternate gene names: 146342284

Gene position: 5626054-5624495 (Counterclockwise)

Preceding gene: 146342285

Following gene: 146342283

Centisome position: 75.45

GC content: 62.31

Gene sequence:

>1560_bases
ATGACACAGTCTGCCGAACATGTGCTCGATCACTTCGAGCTTTTCCGTGGTCCCGAATACCAGCAGATGCTGGCCAACAA
GAAGAAGATGTTCGAAAATCCGCGCGATCCCGCGGAAGTCGAGCGCATCCGCGAATGGGCCAAGACGCCCGAATATCGCG
AGAAGAATTTTGCCCGCGAGGCGCTGACCGTCAACCCGGCCAAGGCCTGCCAGCCGCTCGGCGCCGTGTTCGTCGCCGTC
GGCTTCGAAAAGACCTTGCCGTTCGTGCACGGCTCGCAGGGTTGCGTCGCCTATTATCGCAGCCATCTGTCGCGCCACTT
CAAGGAGCCGAGCTCCTGCGTGTCGTCGTCGATGACCGAGGACGCAGCCGTGTTCGGCGGCCTCAACAACATGATCGACG
GCCTCGCCAACGCCTACAACATGTACAAGCCGAAGATGATCGCGGTGTCGACCACCTGCATGGCGGAAGTCATCGGTGAC
GACCTCAACGCCTTCATCAAGACCTCGAAGGAAAAGGGCTCGGTTCCGGCCGAGTACGACGTTCCCTTCGCTCACACCCC
GGCTTTCGTCGGCAGCCACGTCACCGGCTACGACAACGCGATGAAGGGCATTCTCGACCACTTCTGGGACGGCAAGGCCG
GCACCGCGCCGAAGCTGGAGCGCGTTCCGAACGAGAAGATCAACTTCATCGGCGGCTTCGACGGCTACACCGTCGGCAAC
ATCCGCGAGGTCAAGCACATCTTCGAGGAGATGGGGATCGAGTACACCATCCTCGGCGACAACTCGAATGTGTTCGACAC
GCCGACCGACGGCGAGTTCCGCATGTATGAGGGCGGCACGACCCTGGAAGACGCGGCCAATGCCGTTCACGCCAAGGCGA
CGATCTCGATGCAGCACTATTGCACCGAGAAGACGCTGCCCTTCATCGCCAACCATGGCCAGGAAACGGTCTCGTTCCAT
CACCCGGTCGGCGTCTCCGGCACCGACGCCTTCGTGATGGCGCTGTCGCGCATCTCGGGCAAGGAGATCCCGGAATCGCT
CGCCGTCGAGCGCGGCCGCCTGGTCGACGCGATCGCCGACTCCAGCGCGCACATCCATGGCAAGAAGTTCGCGATCTATG
GCGATCCGGACCTCTGCCTCGGCCTCGCCTCGTTCCTGCTGGAGCTCGGCGCCGAGCCGACCCATGTGCTCGCCACCAAC
GGCAACAAGGAGTGGGCGGAGCGCGTGCAGGCGGTGTTCGATGCCTCGCCGTTCGGCAAGAACTGCCACGTGTATCCCGG
CAAGGATCTCTGGCACATGCGCTCGCTGCTGTTCACCGAGCCGGTCGACTTCCTGATCGGCAACACCTACGGCAAGTACC
TGGAGCGCGACACCGGCACGCCGCTGATCCGCATCGGCTTCCCGATCTTCGATCGCCACCACAAGCATCGCTATCCGGTG
TGGGGCTATCAGGGCGGCCTGAACGTGCTGGTCACGATCCTCGACAAGATCTTCGACGAGATCGATCGCAACACCAACGT
GCCGGCCAAGTCGGACTACAGCTTCGACATCATCCGCTAA

Upstream 100 bases:

>100_bases
TCCAGGCGGCCGAGTAACAACCACCACCCACGGCCTGGCTCTCCTTCGGCGGAGAGCCAGCGCTCCCTGATCGACACAGG
AACATAGAGAGGGTTTTCCC

Downstream 100 bases:

>100_bases
GACACGGCGCGCGGCGGTCCCCAGGGGCCGCCGCGCTTCGCTCCAGACGATGCGGCTCGTGGGAAGGCACCAAGCCTGAG
CCGCCTCGTCCGACCAAGAC

Product: nitrogenase molybdenum-iron protein beta chain, nifK

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MTQSAEHVLDHFELFRGPEYQQMLANKKKMFENPRDPAEVERIREWAKTPEYREKNFAREALTVNPAKACQPLGAVFVAV
GFEKTLPFVHGSQGCVAYYRSHLSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANAYNMYKPKMIAVSTTCMAEVIGD
DLNAFIKTSKEKGSVPAEYDVPFAHTPAFVGSHVTGYDNAMKGILDHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGN
IREVKHIFEEMGIEYTILGDNSNVFDTPTDGEFRMYEGGTTLEDAANAVHAKATISMQHYCTEKTLPFIANHGQETVSFH
HPVGVSGTDAFVMALSRISGKEIPESLAVERGRLVDAIADSSAHIHGKKFAIYGDPDLCLGLASFLLELGAEPTHVLATN
GNKEWAERVQAVFDASPFGKNCHVYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHKHRYPV
WGYQGGLNVLVTILDKIFDEIDRNTNVPAKSDYSFDIIR

Sequences:

>Translated_519_residues
MTQSAEHVLDHFELFRGPEYQQMLANKKKMFENPRDPAEVERIREWAKTPEYREKNFAREALTVNPAKACQPLGAVFVAV
GFEKTLPFVHGSQGCVAYYRSHLSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANAYNMYKPKMIAVSTTCMAEVIGD
DLNAFIKTSKEKGSVPAEYDVPFAHTPAFVGSHVTGYDNAMKGILDHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGN
IREVKHIFEEMGIEYTILGDNSNVFDTPTDGEFRMYEGGTTLEDAANAVHAKATISMQHYCTEKTLPFIANHGQETVSFH
HPVGVSGTDAFVMALSRISGKEIPESLAVERGRLVDAIADSSAHIHGKKFAIYGDPDLCLGLASFLLELGAEPTHVLATN
GNKEWAERVQAVFDASPFGKNCHVYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHKHRYPV
WGYQGGLNVLVTILDKIFDEIDRNTNVPAKSDYSFDIIR
>Mature_518_residues
TQSAEHVLDHFELFRGPEYQQMLANKKKMFENPRDPAEVERIREWAKTPEYREKNFAREALTVNPAKACQPLGAVFVAVG
FEKTLPFVHGSQGCVAYYRSHLSRHFKEPSSCVSSSMTEDAAVFGGLNNMIDGLANAYNMYKPKMIAVSTTCMAEVIGDD
LNAFIKTSKEKGSVPAEYDVPFAHTPAFVGSHVTGYDNAMKGILDHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGNI
REVKHIFEEMGIEYTILGDNSNVFDTPTDGEFRMYEGGTTLEDAANAVHAKATISMQHYCTEKTLPFIANHGQETVSFHH
PVGVSGTDAFVMALSRISGKEIPESLAVERGRLVDAIADSSAHIHGKKFAIYGDPDLCLGLASFLLELGAEPTHVLATNG
NKEWAERVQAVFDASPFGKNCHVYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHKHRYPVW
GYQGGLNVLVTILDKIFDEIDRNTNVPAKSDYSFDIIR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 57763; Mature: 57632

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSAEHVLDHFELFRGPEYQQMLANKKKMFENPRDPAEVERIREWAKTPEYREKNFARE
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
ALTVNPAKACQPLGAVFVAVGFEKTLPFVHGSQGCVAYYRSHLSRHFKEPSSCVSSSMTE
HHCCCHHHHHHHHHHHHHHHCHHHHCCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHCHH
DAAVFGGLNNMIDGLANAYNMYKPKMIAVSTTCMAEVIGDDLNAFIKTSKEKGSVPAEYD
HHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VPFAHTPAFVGSHVTGYDNAMKGILDHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCC
IREVKHIFEEMGIEYTILGDNSNVFDTPTDGEFRMYEGGTTLEDAANAVHAKATISMQHY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEHHHHH
CTEKTLPFIANHGQETVSFHHPVGVSGTDAFVMALSRISGKEIPESLAVERGRLVDAIAD
HHHCCCHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHC
SSAHIHGKKFAIYGDPDLCLGLASFLLELGAEPTHVLATNGNKEWAERVQAVFDASPFGK
CCCEECCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
NCHVYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHKHRYPV
CCEECCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCC
WGYQGGLNVLVTILDKIFDEIDRNTNVPAKSDYSFDIIR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TQSAEHVLDHFELFRGPEYQQMLANKKKMFENPRDPAEVERIREWAKTPEYREKNFARE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
ALTVNPAKACQPLGAVFVAVGFEKTLPFVHGSQGCVAYYRSHLSRHFKEPSSCVSSSMTE
HHCCCHHHHHHHHHHHHHHHCHHHHCCCEECCCHHHHHHHHHHHHHHCCHHHHHHHHCHH
DAAVFGGLNNMIDGLANAYNMYKPKMIAVSTTCMAEVIGDDLNAFIKTSKEKGSVPAEYD
HHHHHCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VPFAHTPAFVGSHVTGYDNAMKGILDHFWDGKAGTAPKLERVPNEKINFIGGFDGYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEECCCCCCCCC
IREVKHIFEEMGIEYTILGDNSNVFDTPTDGEFRMYEGGTTLEDAANAVHAKATISMQHY
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHEEHHHHH
CTEKTLPFIANHGQETVSFHHPVGVSGTDAFVMALSRISGKEIPESLAVERGRLVDAIAD
HHHCCCHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHC
SSAHIHGKKFAIYGDPDLCLGLASFLLELGAEPTHVLATNGNKEWAERVQAVFDASPFGK
CCCEECCCEEEEECCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCC
NCHVYPGKDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRIGFPIFDRHHKHRYPV
CCEECCCCHHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHHCCCCCCCCC
WGYQGGLNVLVTILDKIFDEIDRNTNVPAKSDYSFDIIR
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275 [H]