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The map label for this gene is tktB [C]

Identifier: 146341660

GI number: 146341660

Start: 4958166

End: 4959014

Strand: Reverse

Name: tktB [C]

Synonym: BRADO4764

Alternate gene names: 146341660

Gene position: 4959014-4958166 (Counterclockwise)

Preceding gene: 146341661

Following gene: 146341659

Centisome position: 66.51

GC content: 66.2

Gene sequence:

>849_bases
ATGGACATCGCAACCAATCGGCTCGCCAACGCGCCGACGCTGGCGCAGCGCGCCTACAACATCCGCCGCAACGCGCTGCG
CATGGGCGAGGTGCAGGGGCAGGGCTACATCGCCCAGGCCCTCGACATCTCGGACGTGCTGGCGGTGGCCTATTTCCACG
CCATGCGCTACCGGCCCGAGGATCCCCATTGGGAGGAGCGCGACCGCTTCCTGCTGTCGAACGGCCACTACGCGATCGCG
CTGTATGCCGCGCTGATCGAGGCCGGCATCGTTCCAGAGGCCGAGCTCGAGACTTATGGCAGCGATGATAGCCGGCTGCC
GATGTCGGGCATGGCGTCCTACACGCCGGGCATGGAGATGTCGGGCGGCTCGCTGGGGCTCGGCCTGAGCATCGCCGTCG
GCATGGGTCTCGGGTTGAAGCGCAAGAAGTCCAGCTCCCGCGTCTACACGCTGTTCTCGGACGGCGAGCTCGATGAGGGC
TCGGTGTGGGAGGCGATCTCGTCGGCCAGCCACTACAAGCTCGACAATCTCATCGGCATCGTCGACGTCAACAATCAGCA
GGCCGACGGCCCATCGACCCAGGTGATGGGCTTCGAGCCGCTGGTCGACAGGCTCGAGGCGTTCGGCTGGTTCGTGCAGC
GCATCGACGGCAACGACCTCGATGCTGTGCTGGCCGCCTTCGACGCCGCCAAGAGCCACCCCGAGCCGAAGCCGCGCATG
ATCGTGGCGGACACGCTGATGGGCAAGGGCGTGCCGTTCCTCGAACAACGCGAGAAGAACCATTTCATTCGCGTCGAGCC
GCATGAATGGCAGCTCGCGATTGCCGCACTCGACGCCGGGAGGCCGTGA

Upstream 100 bases:

>100_bases
CATCTACACCTTCCTGGCCTCTGATCTCTCCGCCTACGTCACGGGCGCGGTGATCGATGTCAACGGCGGCATGCTGATCC
ACTAAACAAGGTCTCACACG

Downstream 100 bases:

>100_bases
TGACTTCCAAGAGACCCGCTCCGGATCTGAGCAAGCCGCGCCTGACGACCTCGGCGATGATCGCCTCGATCGCCTCCGAG
GGCCAGCGCACCAAGCCGGC

Product: putative transketolase subunit alpha

Products: D-ribose 5-phosphate; D-xylulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MDIATNRLANAPTLAQRAYNIRRNALRMGEVQGQGYIAQALDISDVLAVAYFHAMRYRPEDPHWEERDRFLLSNGHYAIA
LYAALIEAGIVPEAELETYGSDDSRLPMSGMASYTPGMEMSGGSLGLGLSIAVGMGLGLKRKKSSSRVYTLFSDGELDEG
SVWEAISSASHYKLDNLIGIVDVNNQQADGPSTQVMGFEPLVDRLEAFGWFVQRIDGNDLDAVLAAFDAAKSHPEPKPRM
IVADTLMGKGVPFLEQREKNHFIRVEPHEWQLAIAALDAGRP

Sequences:

>Translated_282_residues
MDIATNRLANAPTLAQRAYNIRRNALRMGEVQGQGYIAQALDISDVLAVAYFHAMRYRPEDPHWEERDRFLLSNGHYAIA
LYAALIEAGIVPEAELETYGSDDSRLPMSGMASYTPGMEMSGGSLGLGLSIAVGMGLGLKRKKSSSRVYTLFSDGELDEG
SVWEAISSASHYKLDNLIGIVDVNNQQADGPSTQVMGFEPLVDRLEAFGWFVQRIDGNDLDAVLAAFDAAKSHPEPKPRM
IVADTLMGKGVPFLEQREKNHFIRVEPHEWQLAIAALDAGRP
>Mature_282_residues
MDIATNRLANAPTLAQRAYNIRRNALRMGEVQGQGYIAQALDISDVLAVAYFHAMRYRPEDPHWEERDRFLLSNGHYAIA
LYAALIEAGIVPEAELETYGSDDSRLPMSGMASYTPGMEMSGGSLGLGLSIAVGMGLGLKRKKSSSRVYTLFSDGELDEG
SVWEAISSASHYKLDNLIGIVDVNNQQADGPSTQVMGFEPLVDRLEAFGWFVQRIDGNDLDAVLAAFDAAKSHPEPKPRM
IVADTLMGKGVPFLEQREKNHFIRVEPHEWQLAIAALDAGRP

Specific function: Unknown

COG id: COG3959

COG function: function code G; Transketolase, N-terminal subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI133778974, Length=252, Percent_Identity=39.2857142857143, Blast_Score=171, Evalue=9e-43,
Organism=Homo sapiens, GI205277463, Length=247, Percent_Identity=39.6761133603239, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4507521, Length=247, Percent_Identity=39.6761133603239, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI225637459, Length=249, Percent_Identity=32.1285140562249, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI225637461, Length=218, Percent_Identity=33.9449541284404, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI225637463, Length=216, Percent_Identity=34.2592592592593, Blast_Score=112, Evalue=5e-25,
Organism=Escherichia coli, GI1788808, Length=251, Percent_Identity=33.4661354581673, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI48994911, Length=244, Percent_Identity=31.9672131147541, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17539652, Length=252, Percent_Identity=34.9206349206349, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6319593, Length=218, Percent_Identity=33.4862385321101, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325331, Length=210, Percent_Identity=37.1428571428571, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI45551847, Length=253, Percent_Identity=34.7826086956522, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI45550715, Length=253, Percent_Identity=34.7826086956522, Blast_Score=134, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24645119, Length=220, Percent_Identity=37.7272727272727, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24666278, Length=252, Percent_Identity=33.7301587301587, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: 2.2.1.1

Molecular weight: Translated: 30967; Mature: 30967

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIATNRLANAPTLAQRAYNIRRNALRMGEVQGQGYIAQALDISDVLAVAYFHAMRYRPE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHCHHHHHHHHHHHHHHCCCC
DPHWEERDRFLLSNGHYAIALYAALIEAGIVPEAELETYGSDDSRLPMSGMASYTPGMEM
CCCHHCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHCCCCCCC
SGGSLGLGLSIAVGMGLGLKRKKSSSRVYTLFSDGELDEGSVWEAISSASHYKLDNLIGI
CCCCCCCHHHEEHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCEECCCEEEE
VDVNNQQADGPSTQVMGFEPLVDRLEAFGWFVQRIDGNDLDAVLAAFDAAKSHPEPKPRM
EECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCE
IVADTLMGKGVPFLEQREKNHFIRVEPHEWQLAIAALDAGRP
EEEEHHHCCCCCHHHHHCCCCEEEECCCCEEEEEEEECCCCC
>Mature Secondary Structure
MDIATNRLANAPTLAQRAYNIRRNALRMGEVQGQGYIAQALDISDVLAVAYFHAMRYRPE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHCHHHHHHHHHHHHHHCCCC
DPHWEERDRFLLSNGHYAIALYAALIEAGIVPEAELETYGSDDSRLPMSGMASYTPGMEM
CCCHHCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCHHCCCCCCC
SGGSLGLGLSIAVGMGLGLKRKKSSSRVYTLFSDGELDEGSVWEAISSASHYKLDNLIGI
CCCCCCCHHHEEHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCEECCCEEEE
VDVNNQQADGPSTQVMGFEPLVDRLEAFGWFVQRIDGNDLDAVLAAFDAAKSHPEPKPRM
EECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCE
IVADTLMGKGVPFLEQREKNHFIRVEPHEWQLAIAALDAGRP
EEEEHHHCCCCCHHHHHCCCCEEEECCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Thiamine diphosphate, mono-or triphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: sedoheptulose 7-phosphate; D-glyceraldehyde 3-phosphate

Specific reaction: sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

General reaction: Keto group transfer [C]

Inhibitor: EDTA; Oxythiamine diphosphate; Phosphate; Rabbit Antibodies; Sulfate [C]

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]