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The map label for this gene is ribH [H]

Identifier: 146341342

GI number: 146341342

Start: 4630134

End: 4630625

Strand: Reverse

Name: ribH [H]

Synonym: BRADO4426

Alternate gene names: 146341342

Gene position: 4630625-4630134 (Counterclockwise)

Preceding gene: 146341343

Following gene: 146341341

Centisome position: 62.1

GC content: 68.09

Gene sequence:

>492_bases
ATGGCAGACGCACGGCGCGCGCCGCTGAAAGACCAGACCGACATCACCGGCGCTCGCGCGCTCATCGTCGAGGCCCGCTT
CTACGACGACATTCAGGACGCGATGCTGGAAGGCGCTACCGCCGAGCTGAAGGCGGCCGGCGTCAGCTACGACGTCCTCA
CCGTCACGGGCTCGTTGGAGATCCCGGCGACGATCGCCATCGCCCTGGATGCGGCCGCCAAGACCGGCAAGCCTTACGAT
CTCGCGATCGCGCTCGGTTGCGTGATCCGCGGTGACACCATCCATTTCGAGATCGTCTCGCAGGAATCCTCCCGCGCGCT
GATGGACCTGTCGGTCGCGCGCGGCCTGCCGCTCGGCAATGGCATTCTCACCGTCAACACCGAGGATCAGGCGTGGGCGC
GTGCCCGCGTCTCCGAGATGAACAAGGGCGGCGATGCCGCCCGCGCTGCGCTCGCCGTGCTGCGCATCAAGCGCCGCCTG
TCGCGAGGCTGA

Upstream 100 bases:

>100_bases
CGCCGTCTCGAACCATGAGGCCCTAATCGATCTCGGCCCTTCGAGGTCAGGCCAAGTGGCCGATCCTCAGGGCGAGGCAG
TTTCAAAACGGATTGTAAAC

Downstream 100 bases:

>100_bases
GATGGCCGAGAACAAACCCTTCAGGGGCCCGATCCGCGCCAACACCGACCGCAAGGCCAACCGCCGCGGCGCCGCGCGCC
TTGCCGCCGTGCAGGCGCTC

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase 1; Lumazine synthase 1; Riboflavin synthase 1 beta chain [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MADARRAPLKDQTDITGARALIVEARFYDDIQDAMLEGATAELKAAGVSYDVLTVTGSLEIPATIAIALDAAAKTGKPYD
LAIALGCVIRGDTIHFEIVSQESSRALMDLSVARGLPLGNGILTVNTEDQAWARARVSEMNKGGDAARAALAVLRIKRRL
SRG

Sequences:

>Translated_163_residues
MADARRAPLKDQTDITGARALIVEARFYDDIQDAMLEGATAELKAAGVSYDVLTVTGSLEIPATIAIALDAAAKTGKPYD
LAIALGCVIRGDTIHFEIVSQESSRALMDLSVARGLPLGNGILTVNTEDQAWARARVSEMNKGGDAARAALAVLRIKRRL
SRG
>Mature_162_residues
ADARRAPLKDQTDITGARALIVEARFYDDIQDAMLEGATAELKAAGVSYDVLTVTGSLEIPATIAIALDAAAKTGKPYDL
AIALGCVIRGDTIHFEIVSQESSRALMDLSVARGLPLGNGILTVNTEDQAWARARVSEMNKGGDAARAALAVLRIKRRLS
RG

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=147, Percent_Identity=40.8163265306122, Blast_Score=86, Evalue=9e-19,
Organism=Saccharomyces cerevisiae, GI6324429, Length=140, Percent_Identity=38.5714285714286, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 17265; Mature: 17134

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADARRAPLKDQTDITGARALIVEARFYDDIQDAMLEGATAELKAAGVSYDVLTVTGSLE
CCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEECCCC
IPATIAIALDAAAKTGKPYDLAIALGCVIRGDTIHFEIVSQESSRALMDLSVARGLPLGN
CCEEEEEEEECHHCCCCCEEEEEEEEEEEECCEEEEEEECCCCCHHHHHHHHHCCCCCCC
GILTVNTEDQAWARARVSEMNKGGDAARAALAVLRIKRRLSRG
CEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADARRAPLKDQTDITGARALIVEARFYDDIQDAMLEGATAELKAAGVSYDVLTVTGSLE
CCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEECCCCEEEEEEEECCCC
IPATIAIALDAAAKTGKPYDLAIALGCVIRGDTIHFEIVSQESSRALMDLSVARGLPLGN
CCEEEEEEEECHHCCCCCEEEEEEEEEEEECCEEEEEEECCCCCHHHHHHHHHCCCCCCC
GILTVNTEDQAWARARVSEMNKGGDAARAALAVLRIKRRLSRG
CEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA