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The map label for this gene is puuR [H]

Identifier: 146341324

GI number: 146341324

Start: 4607336

End: 4607890

Strand: Reverse

Name: puuR [H]

Synonym: BRADO4406

Alternate gene names: 146341324

Gene position: 4607890-4607336 (Counterclockwise)

Preceding gene: 146341326

Following gene: 146341323

Centisome position: 61.8

GC content: 61.26

Gene sequence:

>555_bases
CTGAACATCGGCCCGCGCCTCCGGCGCGCCCGTCTGTCCAAGGGGATGCGCATGCGCGATCTGGCGCAGATCGTCGGTTG
CGACGAGAGCATGATCTCCAAGATCGAGGCCGGCAAGGTGATGCCGTCGCTACCCATGCTCGACAAGATGGTCCACGCGC
TCGACCGCGACATGGCCTCGTTCTTCGGCCTGAGGGTCGACGACTACAAACAGGTGCAGAAGCCCGAGGATCGGATGAAG
GTTTACGTCGATGCGCTGCGCGGCGGCAAGGGCGTCGTCTATGAGCGTCTGGTGCCTGTCGCTGCAGGGAACCTTCTCGA
GGCGAACATTCACGTCGTGGCTCCCGGCGGTGAGAAGATCGACCTCATCACTCATCAGGGCGAGGCCGTCGGCTATCTCA
TCTCCGGAACCATCGAGCTCACCATCGACGACACGACCTATCTCATGACCGCCGGCGACTCTTTTTTTTTCAAGGCGTAT
CTGACAAACAGTTATCGCAACGTCGGAACCGACGAGGCGCGGATCGTCTGGGTCAACACGCCGCAGGTCCACTGA

Upstream 100 bases:

>100_bases
CCGCTTGGTCAGAACAACATGCTGGTAAATCACGTGATTGTTATGCATATTCCGCGGGCTGTAATTGCTGGAAACGCCTC
GCGTGAGCTCGGAAAACACA

Downstream 100 bases:

>100_bases
TGTGTTGAAGGCGCCATCCTGCTTTGGCTTCGACCAGCGCAGGTGAGGGGCCGGTGCATGAATTTCATGCATGTGGCGAG
AACGGGTGCAGTCGACATGG

Product: putative HTH-type transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNIGPRLRRARLSKGMRMRDLAQIVGCDESMISKIEAGKVMPSLPMLDKMVHALDRDMASFFGLRVDDYKQVQKPEDRMK
VYVDALRGGKGVVYERLVPVAAGNLLEANIHVVAPGGEKIDLITHQGEAVGYLISGTIELTIDDTTYLMTAGDSFFFKAY
LTNSYRNVGTDEARIVWVNTPQVH

Sequences:

>Translated_184_residues
MNIGPRLRRARLSKGMRMRDLAQIVGCDESMISKIEAGKVMPSLPMLDKMVHALDRDMASFFGLRVDDYKQVQKPEDRMK
VYVDALRGGKGVVYERLVPVAAGNLLEANIHVVAPGGEKIDLITHQGEAVGYLISGTIELTIDDTTYLMTAGDSFFFKAY
LTNSYRNVGTDEARIVWVNTPQVH
>Mature_184_residues
MNIGPRLRRARLSKGMRMRDLAQIVGCDESMISKIEAGKVMPSLPMLDKMVHALDRDMASFFGLRVDDYKQVQKPEDRMK
VYVDALRGGKGVVYERLVPVAAGNLLEANIHVVAPGGEKIDLITHQGEAVGYLISGTIELTIDDTTYLMTAGDSFFFKAY
LTNSYRNVGTDEARIVWVNTPQVH

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=182, Percent_Identity=30.2197802197802, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20491; Mature: 20491

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIGPRLRRARLSKGMRMRDLAQIVGCDESMISKIEAGKVMPSLPMLDKMVHALDRDMAS
CCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FFGLRVDDYKQVQKPEDRMKVYVDALRGGKGVVYERLVPVAAGNLLEANIHVVAPGGEKI
HHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEE
DLITHQGEAVGYLISGTIELTIDDTTYLMTAGDSFFFKAYLTNSYRNVGTDEARIVWVNT
EEEEECCCEEEEEEEEEEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEECC
PQVH
CCCC
>Mature Secondary Structure
MNIGPRLRRARLSKGMRMRDLAQIVGCDESMISKIEAGKVMPSLPMLDKMVHALDRDMAS
CCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
FFGLRVDDYKQVQKPEDRMKVYVDALRGGKGVVYERLVPVAAGNLLEANIHVVAPGGEKI
HHCCCCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEE
DLITHQGEAVGYLISGTIELTIDDTTYLMTAGDSFFFKAYLTNSYRNVGTDEARIVWVNT
EEEEECCCEEEEEEEEEEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEECC
PQVH
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]