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The map label for this gene is lacF [H]

Identifier: 146341313

GI number: 146341313

Start: 4592711

End: 4593787

Strand: Reverse

Name: lacF [H]

Synonym: BRADO4395

Alternate gene names: 146341313

Gene position: 4593787-4592711 (Counterclockwise)

Preceding gene: 146341314

Following gene: 146341312

Centisome position: 61.61

GC content: 66.3

Gene sequence:

>1077_bases
GTGAGTGCGGCGAACTCACAGCCGGGAATCGTCACGATGCCGGACGCGCCATCGGCTGCAGCGCCGCAGCCCGGCCGCGT
AGCGGGCGCCGTCGGATGGCTCGGCCGCGTCGCGCTACTGCCGGTGGAGCTCGCGCTGCGGCTGCTCGATCCGCCGATGC
GCGCGCTGCAGCGGCTGATCGGCGCGCGCCGCATGGCCTATGCATTCCTGCTGCCCAACTTCGTGTTCTTCGGCCTGTTC
GTGTTCCTGCCGATCGGCATCAACCTGGTGTTCTCGGTGACCGGCGGGGTGGCGCTGTTTCCGTCGCAGCGGCCCTATGT
CGGCGCCGAGCAGTTTTCCTATCTGTTCGACTGCGGCTCCTATCTCGATCCCGCGAGCTGCCGCGAGGATCATTTCTGGC
GCGGCGTCGCCAACACCGTGGAGTTCGTGCTGTTCCAGGTGACGGCGATGGTGCTGCTGTCGCTCGTCACGGCGCTGGTC
CTGAACATGAAGATCCGCGCCCGCGGCTTCTTCCGCGCGGTCTACTTCTTCCCGGTCTTGCTGTCGCCGGTGGTCGTCGC
CCTGATCTGGAAGTGGATCCTGCAGCGCGACGGCCTCCTGAATGCCGCGATCACGACGTTGGGCGGCCAAAAGATCCTGT
TCTTCGTCGACCCCGGCTGGGCGATGTTCTGGGCAGTGTTCGTATCAGTGTGGGCGCATATGGGCTTCTACACACTGATC
CTGCTCGCCGGCCTGCAGGCGATCCCGGCCGATCTCTACGAGGCGGCGGAGATGGACGCGACGCCGCGCTGGCGCGTGCT
GACGCACATCACGCTGCCGCTGCTGTGGCCGAACATGATCGTGGTCATCGTGCTCGCGCTGATCAAGGGCGCGCAGACCT
TCGACGAGGTGTTCGTGCTCACCGGCGGCGGTCCCGGCACGGCCACGCTGATGATCGTGCAGTACATCTACGAGACCGGT
TTCGCCAGCCAGGTGCAGAATTTCGGCCTCGCCGCCGCCGCCTCGGTGGTGCTCGGAATCGTGCTGTTCATCCTGACGCT
GCTGCAATTCGCCGCTGCGCGGCGGAAGGAGGTCTGA

Upstream 100 bases:

>100_bases
GCCGGCTCGGCCAGGCCGTCTCCGGCGAGACGACGCTCGATGAAGCCTACAAGCGCATCACCGCCGACGTCAGCCAGCAG
ATCGCCGAGCGCAACAAGAA

Downstream 100 bases:

>100_bases
GCCATGCGCCTTTTGAGCCTGCTTGGTCAGCGTCGCAGCGGTCCCGGACGAAAGCTGCACTGGACCGACATCGCCGCCTA
CGCCTATCTCGCGCTCGGCG

Product: putative sugar ABC transporter permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSAANSQPGIVTMPDAPSAAAPQPGRVAGAVGWLGRVALLPVELALRLLDPPMRALQRLIGARRMAYAFLLPNFVFFGLF
VFLPIGINLVFSVTGGVALFPSQRPYVGAEQFSYLFDCGSYLDPASCREDHFWRGVANTVEFVLFQVTAMVLLSLVTALV
LNMKIRARGFFRAVYFFPVLLSPVVVALIWKWILQRDGLLNAAITTLGGQKILFFVDPGWAMFWAVFVSVWAHMGFYTLI
LLAGLQAIPADLYEAAEMDATPRWRVLTHITLPLLWPNMIVVIVLALIKGAQTFDEVFVLTGGGPGTATLMIVQYIYETG
FASQVQNFGLAAAASVVLGIVLFILTLLQFAAARRKEV

Sequences:

>Translated_358_residues
MSAANSQPGIVTMPDAPSAAAPQPGRVAGAVGWLGRVALLPVELALRLLDPPMRALQRLIGARRMAYAFLLPNFVFFGLF
VFLPIGINLVFSVTGGVALFPSQRPYVGAEQFSYLFDCGSYLDPASCREDHFWRGVANTVEFVLFQVTAMVLLSLVTALV
LNMKIRARGFFRAVYFFPVLLSPVVVALIWKWILQRDGLLNAAITTLGGQKILFFVDPGWAMFWAVFVSVWAHMGFYTLI
LLAGLQAIPADLYEAAEMDATPRWRVLTHITLPLLWPNMIVVIVLALIKGAQTFDEVFVLTGGGPGTATLMIVQYIYETG
FASQVQNFGLAAAASVVLGIVLFILTLLQFAAARRKEV
>Mature_357_residues
SAANSQPGIVTMPDAPSAAAPQPGRVAGAVGWLGRVALLPVELALRLLDPPMRALQRLIGARRMAYAFLLPNFVFFGLFV
FLPIGINLVFSVTGGVALFPSQRPYVGAEQFSYLFDCGSYLDPASCREDHFWRGVANTVEFVLFQVTAMVLLSLVTALVL
NMKIRARGFFRAVYFFPVLLSPVVVALIWKWILQRDGLLNAAITTLGGQKILFFVDPGWAMFWAVFVSVWAHMGFYTLIL
LAGLQAIPADLYEAAEMDATPRWRVLTHITLPLLWPNMIVVIVLALIKGAQTFDEVFVLTGGGPGTATLMIVQYIYETGF
ASQVQNFGLAAAASVVLGIVLFILTLLQFAAARRKEV

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=305, Percent_Identity=28.8524590163934, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787570, Length=231, Percent_Identity=30.3030303030303, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790465, Length=231, Percent_Identity=30.7359307359307, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 39177; Mature: 39046

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAANSQPGIVTMPDAPSAAAPQPGRVAGAVGWLGRVALLPVELALRLLDPPMRALQRLI
CCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
GARRMAYAFLLPNFVFFGLFVFLPIGINLVFSVTGGVALFPSQRPYVGAEQFSYLFDCGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCCCCCCCHHHHHHHHHCCC
YLDPASCREDHFWRGVANTVEFVLFQVTAMVLLSLVTALVLNMKIRARGFFRAVYFFPVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPVVVALIWKWILQRDGLLNAAITTLGGQKILFFVDPGWAMFWAVFVSVWAHMGFYTLI
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LLAGLQAIPADLYEAAEMDATPRWRVLTHITLPLLWPNMIVVIVLALIKGAQTFDEVFVL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
TGGGPGTATLMIVQYIYETGFASQVQNFGLAAAASVVLGIVLFILTLLQFAAARRKEV
ECCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAANSQPGIVTMPDAPSAAAPQPGRVAGAVGWLGRVALLPVELALRLLDPPMRALQRLI
CCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
GARRMAYAFLLPNFVFFGLFVFLPIGINLVFSVTGGVALFPSQRPYVGAEQFSYLFDCGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCCCCCCCHHHHHHHHHCCC
YLDPASCREDHFWRGVANTVEFVLFQVTAMVLLSLVTALVLNMKIRARGFFRAVYFFPVL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSPVVVALIWKWILQRDGLLNAAITTLGGQKILFFVDPGWAMFWAVFVSVWAHMGFYTLI
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
LLAGLQAIPADLYEAAEMDATPRWRVLTHITLPLLWPNMIVVIVLALIKGAQTFDEVFVL
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
TGGGPGTATLMIVQYIYETGFASQVQNFGLAAAASVVLGIVLFILTLLQFAAARRKEV
ECCCCCHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]