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The map label for this gene is pit [H]

Identifier: 146340685

GI number: 146340685

Start: 3898697

End: 3899704

Strand: Direct

Name: pit [H]

Synonym: BRADO3736

Alternate gene names: 146340685

Gene position: 3898697-3899704 (Clockwise)

Preceding gene: 146340684

Following gene: 146340686

Centisome position: 52.29

GC content: 67.76

Gene sequence:

>1008_bases
GTGGACGCTCAACTCGGCCTGCCCATCCTGGTCGGCCTCATCGCCGTCGCGCTCGCCTTCGACTTCCTGAACGGCCTGCA
CGACGCCGCCAACTCGATCGCGACCATCGTCTCGACCCGCGTGCTGCGGCCGCAATATGCGGTGTTCTGGGCCGCGTTCT
TCAATTTCGTCGCCTTCGGCGTGTTCGGCCTGCATGTCGCGCAGACCATCGGCACCGGGATCATCGACCCCGCGATCGTC
GATGCGCAGGTGATCTTCGCAGCCCTCGTCGGGGCCATCGTCTGGAACCTCGTCACCTGGGCGGCCGGCATTCCCTCGTC
GAGCTCGCACGCGCTGATCGGCGGCCTGGTCGGGGCTGGCGTCGCCAAGGCCGGCATCTCGGCCGCGGTATGGAGCGGCC
TGTCGAAGACGGTGCTGGCGATCGTGCTGTCGCCACTGGTCGGCCTGGTGCTGGCGATGATCCTGGTCGCGATCGTGTCC
TGGGCCTCGGTGCGCTCGACGCCCTTTGCGGTAGATCGCGCCTTCCGCATCCTCCAATTCGCCTCCGCCTCGCTGTACTC
ACTTGGCCATGGCGGCAATGACGCGCAGAAGACGATGGGCATCATCGCTGTGCTGCTGTATTCGCAGGGCCATCTCGGCG
AGCATTTCACGGTGCCGTTCTGGGTCGTGCTGTCGTGCCAGGCGGCCATGGCGCTGGGCACCCTGATGGGCGGTTGGCGC
ATCGTCCGCACCATGGGCCTGCGCATCACCAAGCTGAACCCGATGCAGGGCTTCTGCGCCGAGACCGGCGGCGCCATCAC
CCTGTTCCTGGCGACCTTCCTCGGCGTGCCCGTCTCGACCACCCACACCATCACCGGCGCTATCGTCGGCGTCGGCGCCG
CCCGCCGCGCCTCGGCGGTGCGCTGGAACGTGGCCGGATCGATCGTTTACGCCTGGATCTTCACGATCCCGGCCTCGGCG
ATCGTCTCGGCCGCGACGTTCTGGCTGGTGTCGGTGCTCCGGCACTGA

Upstream 100 bases:

>100_bases
AGATCTACGACCATCTGGAGAAGGTCGCCGACCGCTTCGACGACGTCGCCAACGAGATCAATTCGATCATGATCGAACAG
GTCTAGGGTAGGGCCGCGCC

Downstream 100 bases:

>100_bases
CCCGGTAGGCCGATAGGACAGTGCCTGTAGTTGGGCGGGTTTGGGGCCTTACGGTTCGAGACGCGCCGCAGGGGCGGCGC
TCCTCACCATGAGGGTTGGC

Product: putative low-affinity phosphate transport protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MDAQLGLPILVGLIAVALAFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFVAFGVFGLHVAQTIGTGIIDPAIV
DAQVIFAALVGAIVWNLVTWAAGIPSSSSHALIGGLVGAGVAKAGISAAVWSGLSKTVLAIVLSPLVGLVLAMILVAIVS
WASVRSTPFAVDRAFRILQFASASLYSLGHGGNDAQKTMGIIAVLLYSQGHLGEHFTVPFWVVLSCQAAMALGTLMGGWR
IVRTMGLRITKLNPMQGFCAETGGAITLFLATFLGVPVSTTHTITGAIVGVGAARRASAVRWNVAGSIVYAWIFTIPASA
IVSAATFWLVSVLRH

Sequences:

>Translated_335_residues
MDAQLGLPILVGLIAVALAFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFVAFGVFGLHVAQTIGTGIIDPAIV
DAQVIFAALVGAIVWNLVTWAAGIPSSSSHALIGGLVGAGVAKAGISAAVWSGLSKTVLAIVLSPLVGLVLAMILVAIVS
WASVRSTPFAVDRAFRILQFASASLYSLGHGGNDAQKTMGIIAVLLYSQGHLGEHFTVPFWVVLSCQAAMALGTLMGGWR
IVRTMGLRITKLNPMQGFCAETGGAITLFLATFLGVPVSTTHTITGAIVGVGAARRASAVRWNVAGSIVYAWIFTIPASA
IVSAATFWLVSVLRH
>Mature_335_residues
MDAQLGLPILVGLIAVALAFDFLNGLHDAANSIATIVSTRVLRPQYAVFWAAFFNFVAFGVFGLHVAQTIGTGIIDPAIV
DAQVIFAALVGAIVWNLVTWAAGIPSSSSHALIGGLVGAGVAKAGISAAVWSGLSKTVLAIVLSPLVGLVLAMILVAIVS
WASVRSTPFAVDRAFRILQFASASLYSLGHGGNDAQKTMGIIAVLLYSQGHLGEHFTVPFWVVLSCQAAMALGTLMGGWR
IVRTMGLRITKLNPMQGFCAETGGAITLFLATFLGVPVSTTHTITGAIVGVGAARRASAVRWNVAGSIVYAWIFTIPASA
IVSAATFWLVSVLRH

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=153, Percent_Identity=35.2941176470588, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI5803173, Length=158, Percent_Identity=34.8101265822785, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI1789360, Length=222, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789907, Length=216, Percent_Identity=33.7962962962963, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=31.4814814814815, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71981576, Length=163, Percent_Identity=29.4478527607362, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17536725, Length=179, Percent_Identity=30.1675977653631, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=175, Percent_Identity=29.1428571428571, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32566716, Length=160, Percent_Identity=30, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17557328, Length=166, Percent_Identity=30.7228915662651, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=156, Percent_Identity=28.8461538461538, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356511, Length=193, Percent_Identity=29.5336787564767, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 34883; Mature: 34883

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]