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The map label for this gene is prfC [H]

Identifier: 146340686

GI number: 146340686

Start: 3900053

End: 3901672

Strand: Direct

Name: prfC [H]

Synonym: BRADO3737

Alternate gene names: 146340686

Gene position: 3900053-3901672 (Clockwise)

Preceding gene: 146340685

Following gene: 146340690

Centisome position: 52.3

GC content: 65.06

Gene sequence:

>1620_bases
ATGTCCGATATCGCCGTCACCACCGACCAGCCGTCCCGTTCGCCTCTCGCCGACGAGGTCGCGCGCCGGCGCACCTTCGC
CATCATCTCGCATCCGGACGCCGGCAAGACCACCTTGACCGAGAAGCTGCTGCTGTTCGGCGGAGCCATCAACCTCGCCG
GCCAGGTCAAGGCCAAGGGCGAGCGCCGCAACACCCGCTCTGACTGGATGAAGATCGAGCGCGAGCGCGGCATCTCGGTC
GTCACCTCGGTGATGACCTTCGAGTTCGAAGGGCTGGTGTTCAACCTCCTGGACACGCCGGGCCACGAGGACTTCAGCGA
GGACACGTACCGCACGCTGACCGCGGTCGACTCCGCCGTGATGGTGATCGACGCCGCCAAGGGCATCGAGGCGCGGACGC
GAAAGCTGTTCGAGGTCTGCCGCCTGCGCGACATCCCGATCATCACCTTCATCAACAAGATGGACCGCGAGAGCCGCGAC
ACGTTTGATCTGCTCGACGAGATCGAGAAGACGCTGGCGCTCGACACCACGCCGATGACCTGGCCGGTCGGCCGCGGCCG
CGACTTCATCGGCACCTATGACGTCCGCGATGGCGGCGTGCGCCTGCTCGAAGGTGGCGGCGCCAAAACCGGCGCGGCAC
AGCAGATTGCAATCTCCGAGCTCGGCAAGCTCAACGCCAATCTCGACATGGGCCAGATCACCGACGAGCTCGAGCTCGTC
AAGGAGGCCTGCAAGCCGTTCGAGCTCGACGCCTTTCGCGAGGGTCATCTGACGCCGGTCTATTTCGGCAGCGCGCTGCG
CAATTTCGGCGTCGGCGATCTGCTCGAAGGCCTCGGCCGCTACGCGCCGTCGCCGCGCGCCCAGGATTCAAACCTGCGCA
AGGTCGACGCCGCCGAGCCGCGCATGAGCGCTTTCGTGTTCAAGATCCAGGCGAACATGGACCCGAACCACCGCGACCGC
ATCGCCTTCGCGCGGCTGTGCTCGGGCAAGCTCAACCGTGGCATGAAGGCGAAGCTGGTGCGCACCGGCAAGACCATGCC
GCTGTCGAGCCCGCAATTCTTCTTCGCGCAGGACCGCTCGGTCGCGGACGAGGCTTTCGCCGGCGATGTCGTCGGCATTC
CCAACCACGGTTCGCTTCGCATCGGCGACACGCTGACCGAGGGCGAGGATCTGACCTTCGTCGGCGTTCCCAGCTTTGCC
CCGGAAATCGTCCGCCGCGTTCGGCTCACGGACGCGATGAAGGCGAAGAAGCTCAAGGAGGCCTTGCAGCAGATGTCAGA
GGAGGGCGTCGTACAAGTGTTCCGCCCGCGCGACGGTGCGCCGGCGCTGGTCGGCGTCGTCGGCCCGCTGCAGCTCGACG
TGCTGAAGGCGCGGCTCGATGCCGAGTACTCGTTGCCGGTCGAGTTCGAGATCTCCGAATTCTCGCTGGCGCGCTGGATC
TCCTGCGACGACAAGAAAAAGCTGGAGGCGTTCATCGCCTCCAACAATTCCGGTGTCGCCGACGACGTCGACGGCGATCC
GGTGTTTCTGGCCAAGAACGAGTTCTATCTCGGCTACACCAAGGAACGCGCCGAGGGCATCGTGTTCTCCAACGTCAAGG
ACGTGAAGAAGAAGGCGTGA

Upstream 100 bases:

>100_bases
GGTGCAAAACGCCTATATGGGGGATGCGCGGCGGCCGTGAGCCCGCGCTTTTCCCATGACATCGCCCGCTCTCCGGCCCT
GAAACGACGTCCGCCGCCCC

Downstream 100 bases:

>100_bases
TGAAGGATTATCGCGTTAGTCCAGCGATCCGTCCCCACGCTCTCTCGTCATTCCGGGGCGCGACGAAGTCGCGAGCCCGG
AATCCATTTCGCCGCTCGTG

Product: peptide chain release factor RF-3/GTP-binding factor

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSDIAVTTDQPSRSPLADEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKGERRNTRSDWMKIERERGISV
VTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESRD
TFDLLDEIEKTLALDTTPMTWPVGRGRDFIGTYDVRDGGVRLLEGGGAKTGAAQQIAISELGKLNANLDMGQITDELELV
KEACKPFELDAFREGHLTPVYFGSALRNFGVGDLLEGLGRYAPSPRAQDSNLRKVDAAEPRMSAFVFKIQANMDPNHRDR
IAFARLCSGKLNRGMKAKLVRTGKTMPLSSPQFFFAQDRSVADEAFAGDVVGIPNHGSLRIGDTLTEGEDLTFVGVPSFA
PEIVRRVRLTDAMKAKKLKEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYSLPVEFEISEFSLARWI
SCDDKKKLEAFIASNNSGVADDVDGDPVFLAKNEFYLGYTKERAEGIVFSNVKDVKKKA

Sequences:

>Translated_539_residues
MSDIAVTTDQPSRSPLADEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKGERRNTRSDWMKIERERGISV
VTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESRD
TFDLLDEIEKTLALDTTPMTWPVGRGRDFIGTYDVRDGGVRLLEGGGAKTGAAQQIAISELGKLNANLDMGQITDELELV
KEACKPFELDAFREGHLTPVYFGSALRNFGVGDLLEGLGRYAPSPRAQDSNLRKVDAAEPRMSAFVFKIQANMDPNHRDR
IAFARLCSGKLNRGMKAKLVRTGKTMPLSSPQFFFAQDRSVADEAFAGDVVGIPNHGSLRIGDTLTEGEDLTFVGVPSFA
PEIVRRVRLTDAMKAKKLKEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYSLPVEFEISEFSLARWI
SCDDKKKLEAFIASNNSGVADDVDGDPVFLAKNEFYLGYTKERAEGIVFSNVKDVKKKA
>Mature_538_residues
SDIAVTTDQPSRSPLADEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKGERRNTRSDWMKIERERGISVV
TSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAVMVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESRDT
FDLLDEIEKTLALDTTPMTWPVGRGRDFIGTYDVRDGGVRLLEGGGAKTGAAQQIAISELGKLNANLDMGQITDELELVK
EACKPFELDAFREGHLTPVYFGSALRNFGVGDLLEGLGRYAPSPRAQDSNLRKVDAAEPRMSAFVFKIQANMDPNHRDRI
AFARLCSGKLNRGMKAKLVRTGKTMPLSSPQFFFAQDRSVADEAFAGDVVGIPNHGSLRIGDTLTEGEDLTFVGVPSFAP
EIVRRVRLTDAMKAKKLKEALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYSLPVEFEISEFSLARWIS
CDDKKKLEAFIASNNSGVADDVDGDPVFLAKNEFYLGYTKERAEGIVFSNVKDVKKKA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=476, Percent_Identity=26.2605042016807, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI19923640, Length=500, Percent_Identity=26.4, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=27.9898218829517, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI25306287, Length=472, Percent_Identity=26.4830508474576, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI157426893, Length=153, Percent_Identity=35.9477124183007, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=30.6122448979592, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI4503483, Length=157, Percent_Identity=31.2101910828025, Blast_Score=76, Evalue=9e-14,
Organism=Escherichia coli, GI1790835, Length=528, Percent_Identity=50.5681818181818, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1789738, Length=489, Percent_Identity=25.3578732106339, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=32.7044025157233, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994988, Length=137, Percent_Identity=33.5766423357664, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17533571, Length=492, Percent_Identity=25.2032520325203, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17556745, Length=169, Percent_Identity=36.094674556213, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=169, Percent_Identity=28.9940828402367, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=31.1111111111111, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=31.1111111111111, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=505, Percent_Identity=24.950495049505, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322359, Length=525, Percent_Identity=25.7142857142857, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=29.2993630573248, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=169, Percent_Identity=33.1360946745562, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=169, Percent_Identity=33.1360946745562, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24582462, Length=479, Percent_Identity=25.4697286012526, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI221458488, Length=517, Percent_Identity=25.7253384912959, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=36.3057324840764, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585709, Length=161, Percent_Identity=31.055900621118, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=31.3725490196078, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=31.3725490196078, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21357743, Length=155, Percent_Identity=29.0322580645161, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59484; Mature: 59352

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIAVTTDQPSRSPLADEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKG
CCCCEEECCCCCCCCHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECC
ERRNTRSDWMKIERERGISVVTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAV
CCCCCHHHHHHHHHHCCHHHHHHHHHEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCEE
MVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESRDTFDLLDEIEKTLALDTTPMT
EEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
WPVGRGRDFIGTYDVRDGGVRLLEGGGAKTGAAQQIAISELGKLNANLDMGQITDELELV
CCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KEACKPFELDAFREGHLTPVYFGSALRNFGVGDLLEGLGRYAPSPRAQDSNLRKVDAAEP
HHHCCCCCCCCCCCCCCCCEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RMSAFVFKIQANMDPNHRDRIAFARLCSGKLNRGMKAKLVRTGKTMPLSSPQFFFAQDRS
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCC
VADEAFAGDVVGIPNHGSLRIGDTLTEGEDLTFVGVPSFAPEIVRRVRLTDAMKAKKLKE
HHHHHHCCCEEECCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYSLPVEFEISEFSLARWI
HHHHHHCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHC
SCDDKKKLEAFIASNNSGVADDVDGDPVFLAKNEFYLGYTKERAEGIVFSNVKDVKKKA
CCCCHHHHHHHEECCCCCCCCCCCCCEEEEECCCEEEEECHHHHCCEEECCHHHHHHCC
>Mature Secondary Structure 
SDIAVTTDQPSRSPLADEVARRRTFAIISHPDAGKTTLTEKLLLFGGAINLAGQVKAKG
CCCEEECCCCCCCCHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECC
ERRNTRSDWMKIERERGISVVTSVMTFEFEGLVFNLLDTPGHEDFSEDTYRTLTAVDSAV
CCCCCHHHHHHHHHHCCHHHHHHHHHEEECCEEEEEECCCCCCCCCHHHHHHHHHHCCEE
MVIDAAKGIEARTRKLFEVCRLRDIPIITFINKMDRESRDTFDLLDEIEKTLALDTTPMT
EEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCC
WPVGRGRDFIGTYDVRDGGVRLLEGGGAKTGAAQQIAISELGKLNANLDMGQITDELELV
CCCCCCCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KEACKPFELDAFREGHLTPVYFGSALRNFGVGDLLEGLGRYAPSPRAQDSNLRKVDAAEP
HHHCCCCCCCCCCCCCCCCEEHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
RMSAFVFKIQANMDPNHRDRIAFARLCSGKLNRGMKAKLVRTGKTMPLSSPQFFFAQDRS
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCC
VADEAFAGDVVGIPNHGSLRIGDTLTEGEDLTFVGVPSFAPEIVRRVRLTDAMKAKKLKE
HHHHHHCCCEEECCCCCCEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ALQQMSEEGVVQVFRPRDGAPALVGVVGPLQLDVLKARLDAEYSLPVEFEISEFSLARWI
HHHHHHCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEEECHHHHHHHC
SCDDKKKLEAFIASNNSGVADDVDGDPVFLAKNEFYLGYTKERAEGIVFSNVKDVKKKA
CCCCHHHHHHHEECCCCCCCCCCCCCEEEEECCCEEEEECHHHHCCEEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA