Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 146339912

GI number: 146339912

Start: 3115685

End: 3117106

Strand: Reverse

Name: gatA [H]

Synonym: BRADO2916

Alternate gene names: 146339912

Gene position: 3117106-3115685 (Counterclockwise)

Preceding gene: 146339913

Following gene: 146339911

Centisome position: 41.8

GC content: 68.57

Gene sequence:

>1422_bases
ATGCACAAGCCCGTTTCAGACGACGTCACCTCGTTGCACGATCTCTCCGCCGTAGACCTGATCGCCGGCTACCGCGCGCG
CCAGTTCTCGCCATCCGAAGTGATGGACGACGTGCTGGCGCATGTCGCGGTATGGGAGCCCGACATCAAGGCGCTGTATC
TGCTCGATGTCGATGCCGCGCGGGCCGCGGCCAAGGCATCCACCGAGCGCTGGGTCAATGACGAACCGGCCGGCGTGCTC
GACGGCGTGCCGGCGACGGTGAAGGACAACATTGCCACCAAGGGCCAGCCTATGCCGCTCGGCGCAGCCAGCGTCACGCG
CGCGCCGCTCGCAGCCGACGCGCCGCCCGTGGCGCGTCTGCGCGAGGCCGGCGCGATCATCTTCGCCAAGACCACGATGC
CCGACTACGGCATGCTGTCGTCGGGCCTGTCGAGCTTTCATCCGCTGACGCGCAATCCCTGGGATCTCCGCATGAACCCC
GGCGGCTCCAGCGCCGGTGCGGGTGCCGCGGCTGCAGCAGGCTATGGCCCGCTGCATGTCGGCACCGACATCGGCGGCTC
GATCCGGCTGCCGGCGTCCTGGTGCGGCCTCGTCGGCCTCAAGCCGAGCTTCGGCCGCGTGCCGATCGACCCGCCCTATG
TCGGCCGCGTCGCAGGTCCGATGACGCGCATCGTCGACGACGCCGCGCTGATGATGAGCGTGCTGTCGAAGCCCGACCGC
CGCGACGGCACCAGCCTGCCGCCGTCGGATCTGCACTGGAAGGTCGCCGAGAAGCCGGTGCGCAAGCTCCGCATCGGGCT
GATGCTCGATCTCGGCGGCGTCGGCCAGGCGCTCGAGCAGCCGGTGCGCGCGACGACGCTGGCCGCCGCGAAAGCGTTCG
AGGAGGCCGGCGCCGTGATCACCGAGGTCAAGGGTTTTCTCACCCGCGACATGCTCGACGGCCTCGACAATTTCTGGCGC
GCGCGGCTGTGGGACGATCTCGCCCGGCTCGCGCCGGAGGTGCGCGCGAAGACCCTGCCCTACATTCTGCAATGGGCCGA
GCGCGGCACCGCGCTGTCGGGTGTGGAGGTCGTCAGGGGCTTCAATGTCACCATGGCGCTGCGCGCAGCCGCCGCAAAGC
TGTTCTGCGACTACGACTACATCATCTCGCCGGTTTCGCCGGGCGTGAAGTTTCCCGCCGAATTCGCCTCGCCGGTCAAC
GATCCCGAGAAACCGTTCGAGCACATCACCTACACCGTGCCGTGGAACATGTCGGAGAATCCGGCGATTTCAGTCAATGC
CGGCTACGATCCCGACGGCTTCCCGATCGGCCTGCAGATCGTCGGCCGCCGCTTCGACGATGTCGGCGTGCTCGGCATGG
CCAAGACGTTCGAGAGCCTGCGCGGCGAGCAGCGGCCATGGCCGAAGGCGCCGGCCAAGTGA

Upstream 100 bases:

>100_bases
AAGGATCATCGAGACCCGTCTGGCGGCGGCCTAACGCCGCCTGACGTATGTCAATGAGCGGGCCTGGAATTGAGACGAAC
TTCGGCGACGGAGGACCAGC

Downstream 100 bases:

>100_bases
AGTCTTGTAGCCCGGATGAGCGCAGCGACATCCGGGTTCATCGACACAGCGCGTGATACCCCGCATGTCGCTGCGCTCAT
GCGGGCTACGGACTGCGAAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MHKPVSDDVTSLHDLSAVDLIAGYRARQFSPSEVMDDVLAHVAVWEPDIKALYLLDVDAARAAAKASTERWVNDEPAGVL
DGVPATVKDNIATKGQPMPLGAASVTRAPLAADAPPVARLREAGAIIFAKTTMPDYGMLSSGLSSFHPLTRNPWDLRMNP
GGSSAGAGAAAAAGYGPLHVGTDIGGSIRLPASWCGLVGLKPSFGRVPIDPPYVGRVAGPMTRIVDDAALMMSVLSKPDR
RDGTSLPPSDLHWKVAEKPVRKLRIGLMLDLGGVGQALEQPVRATTLAAAKAFEEAGAVITEVKGFLTRDMLDGLDNFWR
ARLWDDLARLAPEVRAKTLPYILQWAERGTALSGVEVVRGFNVTMALRAAAAKLFCDYDYIISPVSPGVKFPAEFASPVN
DPEKPFEHITYTVPWNMSENPAISVNAGYDPDGFPIGLQIVGRRFDDVGVLGMAKTFESLRGEQRPWPKAPAK

Sequences:

>Translated_473_residues
MHKPVSDDVTSLHDLSAVDLIAGYRARQFSPSEVMDDVLAHVAVWEPDIKALYLLDVDAARAAAKASTERWVNDEPAGVL
DGVPATVKDNIATKGQPMPLGAASVTRAPLAADAPPVARLREAGAIIFAKTTMPDYGMLSSGLSSFHPLTRNPWDLRMNP
GGSSAGAGAAAAAGYGPLHVGTDIGGSIRLPASWCGLVGLKPSFGRVPIDPPYVGRVAGPMTRIVDDAALMMSVLSKPDR
RDGTSLPPSDLHWKVAEKPVRKLRIGLMLDLGGVGQALEQPVRATTLAAAKAFEEAGAVITEVKGFLTRDMLDGLDNFWR
ARLWDDLARLAPEVRAKTLPYILQWAERGTALSGVEVVRGFNVTMALRAAAAKLFCDYDYIISPVSPGVKFPAEFASPVN
DPEKPFEHITYTVPWNMSENPAISVNAGYDPDGFPIGLQIVGRRFDDVGVLGMAKTFESLRGEQRPWPKAPAK
>Mature_473_residues
MHKPVSDDVTSLHDLSAVDLIAGYRARQFSPSEVMDDVLAHVAVWEPDIKALYLLDVDAARAAAKASTERWVNDEPAGVL
DGVPATVKDNIATKGQPMPLGAASVTRAPLAADAPPVARLREAGAIIFAKTTMPDYGMLSSGLSSFHPLTRNPWDLRMNP
GGSSAGAGAAAAAGYGPLHVGTDIGGSIRLPASWCGLVGLKPSFGRVPIDPPYVGRVAGPMTRIVDDAALMMSVLSKPDR
RDGTSLPPSDLHWKVAEKPVRKLRIGLMLDLGGVGQALEQPVRATTLAAAKAFEEAGAVITEVKGFLTRDMLDGLDNFWR
ARLWDDLARLAPEVRAKTLPYILQWAERGTALSGVEVVRGFNVTMALRAAAAKLFCDYDYIISPVSPGVKFPAEFASPVN
DPEKPFEHITYTVPWNMSENPAISVNAGYDPDGFPIGLQIVGRRFDDVGVLGMAKTFESLRGEQRPWPKAPAK

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=494, Percent_Identity=26.9230769230769, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI195972892, Length=501, Percent_Identity=25.748502994012, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI166795287, Length=146, Percent_Identity=41.7808219178082, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17537465, Length=501, Percent_Identity=24.1516966067864, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=179, Percent_Identity=34.6368715083799, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=130, Percent_Identity=41.5384615384615, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556276, Length=326, Percent_Identity=28.8343558282209, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556278, Length=205, Percent_Identity=32.6829268292683, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=279, Percent_Identity=29.0322580645161, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=426, Percent_Identity=23.0046948356808, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=446, Percent_Identity=24.6636771300448, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320448, Length=181, Percent_Identity=38.6740331491713, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=197, Percent_Identity=32.48730964467, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319685, Length=402, Percent_Identity=24.6268656716418, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648113, Length=500, Percent_Identity=26.2, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356731, Length=237, Percent_Identity=32.4894514767932, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI45550774, Length=248, Percent_Identity=32.6612903225806, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648435, Length=248, Percent_Identity=32.6612903225806, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648437, Length=248, Percent_Identity=32.6612903225806, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078093, Length=511, Percent_Identity=23.2876712328767, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648441, Length=168, Percent_Identity=38.0952380952381, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648439, Length=168, Percent_Identity=38.0952380952381, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24644968, Length=508, Percent_Identity=24.4094488188976, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24652985, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922090, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652981, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24652983, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 50632; Mature: 50632

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKPVSDDVTSLHDLSAVDLIAGYRARQFSPSEVMDDVLAHVAVWEPDIKALYLLDVDAA
CCCCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECHHH
RAAAKASTERWVNDEPAGVLDGVPATVKDNIATKGQPMPLGAASVTRAPLAADAPPVARL
HHHHHHHHHHCCCCCCCCHHCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
REAGAIIFAKTTMPDYGMLSSGLSSFHPLTRNPWDLRMNPGGSSAGAGAAAAAGYGPLHV
HHCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCCEEE
GTDIGGSIRLPASWCGLVGLKPSFGRVPIDPPYVGRVAGPMTRIVDDAALMMSVLSKPDR
CCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RDGTSLPPSDLHWKVAEKPVRKLRIGLMLDLGGVGQALEQPVRATTLAAAKAFEEAGAVI
CCCCCCCCCCCEEHHHHHHHHHHHHHHEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
TEVKGFLTRDMLDGLDNFWRARLWDDLARLAPEVRAKTLPYILQWAERGTALSGVEVVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
FNVTMALRAAAAKLFCDYDYIISPVSPGVKFPAEFASPVNDPEKPFEHITYTVPWNMSEN
CCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHCCCCCCCCCHHHHEEEEECCCCCCC
PAISVNAGYDPDGFPIGLQIVGRRFDDVGVLGMAKTFESLRGEQRPWPKAPAK
CEEEEECCCCCCCCCEEHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MHKPVSDDVTSLHDLSAVDLIAGYRARQFSPSEVMDDVLAHVAVWEPDIKALYLLDVDAA
CCCCCCCHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECHHH
RAAAKASTERWVNDEPAGVLDGVPATVKDNIATKGQPMPLGAASVTRAPLAADAPPVARL
HHHHHHHHHHCCCCCCCCHHCCCCCHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHH
REAGAIIFAKTTMPDYGMLSSGLSSFHPLTRNPWDLRMNPGGSSAGAGAAAAAGYGPLHV
HHCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCCEEE
GTDIGGSIRLPASWCGLVGLKPSFGRVPIDPPYVGRVAGPMTRIVDDAALMMSVLSKPDR
CCCCCCCEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
RDGTSLPPSDLHWKVAEKPVRKLRIGLMLDLGGVGQALEQPVRATTLAAAKAFEEAGAVI
CCCCCCCCCCCEEHHHHHHHHHHHHHHEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
TEVKGFLTRDMLDGLDNFWRARLWDDLARLAPEVRAKTLPYILQWAERGTALSGVEVVRG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
FNVTMALRAAAAKLFCDYDYIISPVSPGVKFPAEFASPVNDPEKPFEHITYTVPWNMSEN
CCHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHCCCCCCCCCHHHHEEEEECCCCCCC
PAISVNAGYDPDGFPIGLQIVGRRFDDVGVLGMAKTFESLRGEQRPWPKAPAK
CEEEEECCCCCCCCCEEHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA